An <i>Mspl</i> polymorphism in the hyman serotonin receptor gene (HTR2): detection by DGGE and RFLP analysis

Warren, James T.; Peacock, Michael L.; Rodríguez, Luis Carbajal; Fink, John K.

doi:10.1093/hmg/2.3.338

Cited by 166 publications

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“…The T102C polymorphism is silent and does not result in an alteration of the amino acid sequence of the 5-HT 2A R (27). However, the T102C polymorphism is located in exon 1 near the promoter region and may therefore play a role in gene regulation and expression (28).…”

Section: Discussionmentioning

confidence: 99%

Sensorimotor Gating of Schizophrenia Patients Is Influenced by 5-HT2A Receptor Polymorphisms

Quednow

Kühn

Mößner

et al. 2008

Biological Psychiatry

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Section: Discussionmentioning

confidence: 99%

Sensorimotor Gating of Schizophrenia Patients Is Influenced by 5-HT2A Receptor Polymorphisms

Quednow

Kühn

Mößner

et al. 2008

Biological Psychiatry

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“…The silent T → C 102 polymorphism at the HTR2A locus was genotyped employing a modified protocol of Warren et al (1993). Primer sequences were the same as those …”

Section: Laboratory Methodsmentioning

confidence: 99%

Serotonin Subtype 2 Receptor Genes and Clinical Response to Clozapine in Schizophrenia Patients

Masellis

Basile

Meltzer

et al. 1998

Neuropsychopharmacology

227

135

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“…Genotyping of the T102C polymorphism was performed by the polymerase chain reaction (PCR) method proposed by Warren et al (11), with minor modifications performed by do Prado-Lima et al (15). Briefly, standard PCR was carried out in a 25-µL volume containing 100 ng genomic DNA, 200 µM of each dNTP, 1.5 mM MgCl 2 , 250 nM of the sense (5'-TGTGCTACAAG TTCTGGCTT-3') and anti-sense (5'-GTGCA GTTTTTCT CTAGGG-3') primers, and 0.6 U DNA Taq polymerase.…”

Section: Molecular Analysismentioning

confidence: 99%

Is there an association between T102C polymorphism of the serotonin receptor 2A gene and urinary incontinence?

Schwanke

Bittencourt

Noronha

et al. 2007

Braz J Med Biol Res

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The regulation of bladder function is influenced by central serotonergic modulation. Several genetic polymorphisms related to serotonin control have been described in the literature. T102C polymorphism of the serotonin receptor 2A gene (5-HT2A) has been shown to be associated with certain diseases such as non-fatal acute myocardial infarction, essential hypertension, and alcoholism. In the present study, we examined the association between 5-HT2A gene polymorphism and urinary incontinence in the elderly. A case-control study was performed in 298 elderly community dwellers enrolled in the Gravataí-GENESIS Project, Brazil, which studies gene-environmental interactions in aging and age-related diseases. Clinical, physical, biochemical, and molecular analyses were performed on volunteers. 5-HT2A genotyping was determined by PCR-RFLP techniques using the HpaII restriction enzyme. The subjects had a mean age of 68.05 ± 6.35 years (60-100 years), with 16.9% males and 83.1% females. The C allele frequency was 0.494 and the T allele frequency was 0.506. The CC genotype frequency was 21.78%, the CT genotype frequency was 55.24% and the TT genotype frequency was 22.98%. We found an independent significant association between the TT genotype (35.7%) and urinary incontinence (OR = 2.06, 95%CI = 1.16-3.65). Additionally, urinary incontinence was associated with functional dependence and systolic hypertension. The results suggest a possible genetic influence on urinary incontinence involving the serotonergic pathway. Further investigations including urodynamic evaluation will be performed to better explain our findings.

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An Mspl polymorphism in the hyman serotonin receptor gene (HTR2): detection by DGGE and RFLP analysis

Cited by 166 publications

References 0 publications

Sensorimotor Gating of Schizophrenia Patients Is Influenced by 5-HT2A Receptor Polymorphisms

Sensorimotor Gating of Schizophrenia Patients Is Influenced by 5-HT2A Receptor Polymorphisms

Serotonin Subtype 2 Receptor Genes and Clinical Response to Clozapine in Schizophrenia Patients

Is there an association between T102C polymorphism of the serotonin receptor 2A gene and urinary incontinence?

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