An in vitro focus on doxorubicin hydrochloride delivery of novel pH‐responsive poly(2‐succinyloxyethylmethacrylate) and poly[(N‐4‐vinylbenzyl),N,N‐diethylamine] diblock copolymers

Abstract

Novel pH‐responsive poly(2‐succinyloxyethylmethacrylate)‐b‐poly[(N‐4‐vinylbenzyl),N,N‐diethylamine] [poly(SEMA‐b‐VEA)] diblock copolymers were synthesized via reversible addition fragmentation transfer (RAFT) polymerization to investigate their self‐assembly micellar behavior. The self‐assembly behaviors of synthesized diblock copolymers with distinct molecular weights (labeled (1) to) were confirmed by 1H NMR spectroscopy, TEM and dynamic light scattering measurements. Doxorubicin hydrochloride (DOX) loading capacity was evaluated, and the in vitro cytotoxicity effect of DOX‐loaded diblock copolymer was also studied by assessing the survival rate of the breast cancer cell line MCF‐7 with 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. The results exhibited remarkable controlled release in the MTT assay. The DOX encapsulation efficiency was calculated to be 96.4%. The size and zeta potential of DOX‐loaded poly(SEMA‐b‐VEA) diblock copolymers were 204 nm and +5.7 mV at a pH of 7.4. DOX release values after 440 h at pH 7.4, 5.4 and 4 were 22.15%, 31.43% and 47.06%, respectively. The released values of DOX‐loaded poly(SEMA‐b‐VEA) and at pH 7.4 were 22.15%, 20.5% and 17.5%, respectively. Cell survival ratios were 18.9%, 23.16% and 16.92% after 72 h. Poly(SEMA‐b‐VEA) copolymers can be considered in nanomedicine applications due to their excellent pH‐responsive micellar behavior. © 2017 Society of Chemical Industry

Designing polymers for cartilage uptake: effects of architecture and molar mass

“…For preparation of the linear polymers, 4-cyanopentanoic acid dithiobenzoate (CTP) was synthesized as previously reported. 43…”

“…For preparation of the linear polymers, 4-cyanopentanoic acid dithiobenzoate (CTP) was synthesized as previously reported. 43 The 4-arm CTA comprised CTP conjugated to pentaerythritol with a thioether spacer. The spacer was necessary, as attempts to directly conjugate CTP to pentaerythritol were unsuccessful.…”

A perfect stimuli-responsive magnetic nanocomposite for intracellular delivery of doxorubicin

“…The several stimuli-responsive polymers targeted to release the encapsulated therapeutics in respond to several endogenous (pH value, redox and enzyme) and exogenous (temperature, light, etc.) stimuli have been developed by incorporating different responsive building blocks into polymers [12][13][14][15][16]. Among all stimuli, pH sensitivity is the most investigated one, owing to the pH value gradients found within the physiological environment.…”