An <i>in vitro</i> comparison of the bactericidal efficacy of lethal photosensitization or sodium hyphochlorite irrigation on <i>Streptococcus intermedius</i> biofilms in root canals

Seal, G. J.; Ng, YL; Spratt, DA; Bhatti, Manpreet; Gulabivala, K

doi:10.1046/j.1365-2591.2002.00477.x

Cited by 123 publications

(130 citation statements)

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“…14 Several studies have shown that oral bacteria are sensitive to photodynamic therapy (PDT). 15,16 In recent years, in vitro [17][18][19][20][21][22][23][24][25][26] and in vivo [27][28][29] studies have been performed to investigate the antibacterial effect of the PDT on the microorganisms in root canal. Most of these studies have focused on the PDT capability as a complementary technique besides the conventional endodontic treatment.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions

et al. 2016

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IntroductionMicrobial infection plays an important role in persistent periapical lesions.1 Insufficient disinfection of root canals could lead to treatment failure and persistent periapical pathology. 2Conventional techniques of root canal treatments such as mechanical instrumentation and chemical debridement with antimicrobial irrigants, such as sodium hypochlorite (NaOCl), chlorhexidine and calcium hydroxide do not always suffice to predictably render root canals free of bacteria.3,4 Factors such as anatomical complexities, bacterial growth as biofilm, render complete disinfection of the root canal system almost impossible. 5Studies have revealed a success rate of 94% with a negative culture before obturation has decreased to 68%, with a positive culture.2 Resolution of periapical lesions is more achievable when there is negative culture before obturation. 6Cross sectional studies have reported 20%-60% of treatment failure and 52% of endodontic failure in Iranian population.7-10 Existence of intracanal microorganisms would lessen the treatment outcomes of endodontic re-treatment comparing to primary endodontic treatment. 2Based on the previous studies, bacterial microflora in teeth with failed endodontic treatment is different from the microflora of the primary endodontic infection. All culture and PCR based methods have shown that primary endodontic infection is a polymicrobial infection involving fully anaerobic microorganisms, 5,11 while the failure AbstractIntroduction: The aim of this study was to compare the antibacterial efficacy of diode laser 810nm and photodynamic therapy (PDT) in reducing bacterial microflora in endodontic retreatment of teeth with periradicular lesion. Methods: In this in vivo clinical trial, 20 patients who needed endodontic retreatment were selected. After conventional chemo mechanical preparation of root canals, microbiological samples were taken with sterile paper point (PP), held in thioglycollate broth, and then were transferred to the microbiological lab. In the first group, PDT with methylene blue (MB) and diode laser (810 nm, 0.2 W, 40 seconds) was performed and in the second group diode laser (810 nm, 1.2 W, 30 seconds) was irradiated. Then second samples were taken from all canals. Results: CFU/ml amounts showed statistically significant reduction in both groups (P < 0.001). CFU/ml amounts were compared between the two groups and there was no statistical difference. Conclusion: PDT and diode laser 810 nm irradiation are effective methods for root canal disinfection. PDT is a suitable alternative for diode laser 810 nm irradiation, because of lower thermal risk on root dentin.

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Section: Introductionmentioning

confidence: 99%

Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions

et al. 2016

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“…In this therapy, certains dyes such as Toluidine blue, Methylene blue, Azuline paste, Tolonium chloride and light source such as Diode laser, 35-mW helium-neon low power laser [15], Gallium-aluminium arsenide (Ga-Al-As) diode laser can be used.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Photodynamic Therapy (PDT) in Disinfection of Deeper Dentinal Tubules in a Root Canal System: An In Vitro Study

Bumb

Bhaskar

Agali

et al. 2014

JCDR

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Context: The success of endodontic treatment therapy depends on how well we eliminate pathogenic microflora from the root canal system as micro organism as the major cause of root canal infection. Conventional root canal treatment can fail if microorganisms cannot be removed sufficiently by thorough cleaning, shaping of root canal. Newer modalities such as photodynamic therapy are being tried now a days for disinfection of root canals. Aim & Objectives:The basic aim of this study was assessment of the antimicrobial efficacy of Photodynamic Therapy in deeper dentinal tubules for effective disinfection of root canals using microbiological and scanning electron microscopic examination in vitro. Materials and Methods:The study was conducted at Teerthanker Mahaveer Dental College & Research Centre. The teeth required for study was collected from Department of Oral and Maxillofacial Surgery. Only freshly extracted 20 intact, non carious single rooted teeth which were indicated for orthodontic treatment were taken for this study. Statistical analysis was done using Student's Unpaired t-test were at (p<0.001) was found to be highly significant. Microbiological examination of samples were done and colony forming units were counted to assess the disinfection potential of photodynamic therapy. Scanning electron microscopic examination of samples was done to check penetration of bacteria's into deeper dentinal tubules.Results: On examination, there was a marked reduction in microbial growth after use of photodynamic therapy. On scanning electron microscopic examination, it was observed that there were less number of bacteria's in deeper dentinal tubules in case of PDT group as compared to control group. Conclusion:The results of the present study indicate that PDT can be effectively used during antimicrobial procedures along with conventional disinfection procedure for sterilization of root canals.

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“…However, common sense dictates that higher concentrations will achieve reduction in bacterial load faster owing to the presence of a higher concentration of chloramine molecules.It should also be kept in mind that the more concentrated solutions of NaOCl are thicker and subsequently there is less wetting of the canal walls. (16) It has been observed that NaOCl solutions at concentrations of 0.5%, 3% and 5% degrade the organic phase (collagen) of dentine with no loss of mineral, possibly leading to brittleness of endodontically treated teeth. (14) There are significant biological toxicity risks if NaOCl is expressed under pressure into the periodontal ligament space.…”

Section: Sodium Hypochloritementioning

confidence: 99%

Antimicrobial Irrigants in the Endodontic Therapy

Iqbal¹

2012

IJHS

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Abstract:This paper highlights the importance of root canal disinfection. It discusses the different endodontic irrigants available and comments on how these can be used most effectively. Eliminating bacteria from the root canal system is an essential stage in endodontic therapy. An objective of endodontic treatment is removal of diseased tissue, elimination of bacteria from the canal system and prevention of recontamination.(1) Disinfection of the root canal system, as part of endodontic therapy, by preparation and irrigation is a key in reducing the number of bacteria within the root canal and helping to control periapical disease.

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An in vitro comparison of the bactericidal efficacy of lethal photosensitization or sodium hyphochlorite irrigation on Streptococcus intermedius biofilms in root canals

Abstract: The combined use of a photosensitizing agent and a low power laser directed at the access cavity was bactericidal to S. intermedius biofilms in root canals but was unable to achieve total kill, unlike 3% NaOCl.

Cited by 123 publications

References 48 publications

Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions

Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions

Assessment of Photodynamic Therapy (PDT) in Disinfection of Deeper Dentinal Tubules in a Root Canal System: An In Vitro Study

Antimicrobial Irrigants in the Endodontic Therapy

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