An HPLC-DAD method for the simultaneous determination of nine β-lactam antibiotics in ewe milk

Cámara, Marı́a S.; Gallego-Picó, Alejandrina; Martínez, Rosa María Goig; Fernández-Hernando, P.; Durand-Alegría, J.S.; Sánchez, Pedro José González-Trevijano

doi:10.1016/j.foodchem.2013.02.131

Cited by 71 publications

(19 citation statements)

References 31 publications

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“…The determination of cephalexin at this level requires sensitive analytical methods. The traditional approach for the detection of CFX residues in milk includes use of high-performance liquid chromatography (Oliveira et al 2007;Cámara et al 2013;Panda et al 2013), highperformance thin layer chromatography (Agbaba et al 1998), mass spectrometry (Wu et al 2010) and liquid chromatography-mass spectrometry (Wu et al 2010;Liu et al 2014). Although these methods are sensitive, specific and reliable for quantification, they are time-consuming, expensive and are not applicable under field conditions.…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Lata

Sharma

Naik

et al. 2015

Journal of Food Quality

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A rapid and semiquantitative lateral flow assay has been developed for detection of cephalexin (CFX) in milk. The assay is based on a competitive format using polyclonal antibodies. Antibodies were raised in rabbits against CFX-keyhole limpet hemocyanin. The specificity of antibodies was ascertained by direct enzyme-linked immunosorbent assay. The laboratory prepared gold nanoparticles coupled to anti-CFX antibodies, which competed for free CFX in sample and CFX-bovine serum albumin conjugate on the test line of a lateral flow strip. The control line of the lateral flow strip consisted of species-specific antibody. The assay was validated with spiked milk samples and involved application of skim milk to the adsorbent pad followed by visualization of red color on test and control lines within 8 min. The lower detection limit of CFX was 30 ppb in milk, which is lower than the Codex prescribed maximum residue limit. The storage stability of the prepared strip was also established. PRACTICAL APPLICATIONSA simple method for the detection of cephalexin in milk using lateral flow assay has been described. The applicability of polyclonal antibodies in the development of lateral flow assay has been demonstrated. The method is sensitive to detect 30 ppb cephalexin in milk, which is lower than the Codex prescribed maximum residue limit of 100 ppb. This method can be used for preliminary screening of milk samples for the presence of cephalexin residues in milk.

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Section: Introductionmentioning

confidence: 99%

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Lata

Sharma

Naik

et al. 2015

Journal of Food Quality

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“…The contents were centrifuged (1123g, 20 min, 4°C) and supernatant was collected and subsequently passed through 0.22 l membrane filter. Five millilitres of filtrate was loaded on (i) SPE cartridges containing imprinted or NIP and (ii) 6 ml reversed-phase DSC-18 LT SPE cartridge (Camara et al, 2013). Prior to loading, the cartridges were sequentially conditioned with 12 ml methanol and 12 ml water.…”

Section: Spe Of Cfx From Milk and Subsequent Analysis By Hplcmentioning

confidence: 99%

Synthesis and application of cephalexin imprinted polymer for solid phase extraction in milk

et al. 2015

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“…In the field of separation techniques, LC (Liquid Chromatography) is the most frequently applied one, usually followed by either diode array UltraViolet (UV) detection [ 2 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ] or mass spectrometry [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Prior to chromatographic analysis, a sample pretreatment technique should be applied for the effective isolation of antibiotics from complex milk matrix. Sample preparation techniques commonly used include extraction of target analytes with a variety of organic solvents followed by SPE (Solid Phase Extraction) [ 10 , 18 ], solid phase microextraction [ 14 ] and liquid–liquid extraction [ 11 , 16 , 19 , 20 ]. During recent years, modern extraction techniques are being used for the same purpose, including solid phase extraction using molecularly imprinted polymers as the sorbent material [ 9 , 12 , 17 , 21 ], matrix solid-phase dispersion (MSPD), and dispersive SPE applying the quick, easy, cheap, effective, rugged, and safe (QuEChERS) methodology [ 2 , 7 , 8 , 13 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of β-Lactams and Chloramphenicol Residues in Raw Milk—Development and Application of an HPLC-DAD Method in Comparison with Microbial Inhibition Assays

Karageorgou¹,

Christoforidou²,

Ioannidou³

et al. 2018

Foods

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The present study was carried out to assess the detection sensitivity of four microbial inhibition assays (MIAs) in comparison with the results obtained by the High Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) method for antibiotics of the β-lactam group and chloramphenicol in fortified raw milk samples. MIAs presented fairly good results when detecting β-lactams, whereas none were able to detect chloramphenicol at or above the permissible limits. HPLC analysis revealed high recoveries of examined compounds, whereas all detection limits observed were lower than their respective maximum residue limits (MRL) values. The extraction and clean-up procedure of antibiotics was performed by a modified matrix solid phase dispersion procedure using a mixture of Plexa by Agilent and QuEChERS as a sorbent. The HPLC method developed was validated, determining the accuracy, precision, linearity, decision limit, and detection capability. Both methods were used to monitor raw milk samples of several cows and sheep, obtained from producers in different regions of Greece, for the presence of examined antibiotic residues. Results obtained showed that MIAs could be used effectively and routinely to detect antibiotic residues in several milk types. However, in some cases, spoilage of milk samples revealed that the kits’ sensitivity could be strongly affected, whereas this fact does not affect the effectiveness of HPLC-DAD analysis.

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An HPLC-DAD method for the simultaneous determination of nine β-lactam antibiotics in ewe milk

Cited by 71 publications

References 31 publications

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Lateral Flow Assay–Based Rapid Detection of Cephalexin in Milk

Synthesis and application of cephalexin imprinted polymer for solid phase extraction in milk

Detection of β-Lactams and Chloramphenicol Residues in Raw Milk—Development and Application of an HPLC-DAD Method in Comparison with Microbial Inhibition Assays

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