An HcpR paralog ofDesulfovibrio gigas provides protection against nitrosative stress

Abstract

HighlightsDesulfovibrio gigas genome encodes two HcpR paralogs, HcpR1 and HcpR2.Cells lacking HcpR1 are less tolerant to NO.HcpR1 regulates the expression of several genes related to nitrogen metabolism.Phylogenetic analyses indicate that the presence of HcpR paralogs is a common finding among Desulfovibrio species.

Anaerobic bacterial response to nitric oxide stress: Widespread misconceptions and physiologically relevant responses

“…Similar results were obtained by Boutrin et al. (2012) for Porphyromonas gingivalis , and da Silva et al (2015) showed that a Desulfovibrio gigas hcpR mutant that cannot produce Hcp is sensitive to nitrosative stress.…”

“…Transcription factors that respond directly and with high sensitivity to NO fall into two classes: hemoproteins; and nonheme iron or iron‐sulfur proteins (Rodionov et al., 2005). Typical of the former group are the HcpR proteins of sulfate reducing bacteria and Porphyromonas gingivalis (Belvin et al., 2019; Cadby et al., 2016; da Silva et al., 2015). The second group includes NsrR proteins found in a wide range of β, γ, and δ proteobacteria, the enhancer binding protein, NorR, and other HcpR proteins (Crack et al, 2011; D’Autréaux et al, 2005; Isabella et al., 2009; Pohlmann et al., 2000; Yuki et al., 2008).…”

Hydrogenases and H2 metabolism in sulfate-reducing bacteria of the Desulfovibrio genus

“…Several Crp/Fnr homologues have been identified in Desulfovibrio species, such as HcpR which has been proposed to have a protective role against nitrosative stress in D. gigas (da Silva et al, 2015;Rodionov, Dubchak, Arkin, Alm, & Gelfand, 2004). In 2012, Zhou et al investigated the role of four different transcriptional regulators of the Crp/Fnr family in D. vulgaris.…”

Regulation, sensory domains and roles of two Desulfovibrio desulfuricans ATCC27774 Crp family transcription factors, HcpR1 and HcpR2, in response to nitrosative stress

“…Induction of hcp in response to nitrite or NO in D. vulgaris , D. gigas and P. gingivalis is HcpR‐dependent (Lewis et al ., ; Zhou et al . ; da Silva et al ., ). The in vitro binding of P. gingivalis HcpR to hcp promoter DNA was demonstrated to be heme‐dependent, but the HcpR of this species has limited sequence similarity to those of proteobacteria such as Desulfovibrio species (Lewis et al ., ).…”