Hydrogenases and H2 metabolism in sulfate-reducing bacteria of the Desulfovibrio genus

Baffert, Carole; Kpebe, Arlette; Avilán, Luisana; Brugna, Myriam

doi:10.1016/bs.ampbs.2019.03.001

Cited by 39 publications

(32 citation statements)

References 189 publications

(210 reference statements)

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“…Alternatively, sulfate reducing bacteria such as Desulfovibrio sp. are metabolically highly versatile since they can use sulfate as terminal electron acceptor in the oxidation of H2 or organic compounds, or also produce H2 when growing fermentatively in the absence of sulfate and in syntrophy with formate/H2-consuming methanogens (Pereira et al, 2008;Plugge et al, 2011;Baffert et al, 2019). They exhibit a hight content and diversity of Mo-formate dehydrogenases, W-formate dehydrogenases (Maia et al, 2017;Nielsen et al, 2019;Niks and Hille, 2019) as well as both [FeFe]-and [NiFe]-hydrogenases (Martins et al, 2016;Baffert et al, 2019) which share the same electron acceptor, the small tetraheme cytochrome c3 (Matias et al, 2005;da Silva et al, 2012).…”

Section: Whole Cell Biocatalysis Of Formate Production: What's Next?mentioning

confidence: 99%

“…are metabolically highly versatile since they can use sulfate as terminal electron acceptor in the oxidation of H2 or organic compounds, or also produce H2 when growing fermentatively in the absence of sulfate and in syntrophy with formate/H2-consuming methanogens (Pereira et al, 2008;Plugge et al, 2011;Baffert et al, 2019). They exhibit a hight content and diversity of Mo-formate dehydrogenases, W-formate dehydrogenases (Maia et al, 2017;Nielsen et al, 2019;Niks and Hille, 2019) as well as both [FeFe]-and [NiFe]-hydrogenases (Martins et al, 2016;Baffert et al, 2019) which share the same electron acceptor, the small tetraheme cytochrome c3 (Matias et al, 2005;da Silva et al, 2012). Hence, it was recently demonstrated that bacterium from the Desulfovibrio genus can produce formate from H2 and CO2 (da Silva et al, 2013;Mourato et al, 2017), involving the periplasmic HydAB [FeFe]-hydrogenase (in H2-oxidation mode) and the cytoplasmic Mo-formate dehydrogenase enzyme, both most likely wired via the periplasmic tetraheme cytochrome c3 network (Mourato et al, 2017).…”

Section: Whole Cell Biocatalysis Of Formate Production: What's Next?mentioning

confidence: 99%

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HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases

Roger

Reed

Sargent

2021

Preprint

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ABSRACTEscherichia coli is gram-negative bacterium that is a workhorse of the biotechnology industry. The organism has a flexible metabolism and can perform a mixed-acid fermentation under anaerobic conditions. Under these conditions E. coli synthesises a formate hydrogenlyase isoenzyme (FHL-1) that can generate molecular hydrogen and carbon dioxide from formic acid. The reverse reaction is hydrogen-dependent carbon dioxide reduction (HDCR), which has exciting possibilities in bio-based carbon capture and storage if it can be harnessed. In this study, an E. coli host strain was optimised for the production of formate from H2 and CO2 during bacterial growth in a pressurised batch bioreactor. A host strain was engineered that constitutively produced the FHL-1 enzyme and incorporation of tungsten in to the enzyme, in place of molybdenum, helped poise the reaction in the HDCR direction. The engineered E. coli strain showed an ability to grow under fermentative conditions while simultaneously producing formate from gaseous H2 and CO2 supplied in the bioreactor. However, while a sustained pressure of 10 bar N2 had no adverse effect on cell growth, when the culture was placed at or above 4 bar pressure of a H2:CO2 mixture then a clear growth deficiency was observed. Taken together, this work demonstrates that growing cells can be harnessed to hydrogenate carbon dioxide and provides fresh evidence that the FHL-1 enzyme may be intimately linked with bacterial energy metabolism.

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Section: Whole Cell Biocatalysis Of Formate Production: What's Next?mentioning

confidence: 99%

Section: Whole Cell Biocatalysis Of Formate Production: What's Next?mentioning

confidence: 99%

HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases

Roger

Reed

Sargent

2021

Preprint

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“…However, when sulfate is limited, many SRB species grow fermentatively. In that case, they usually require methanogen or other hydrogen-scavengers to make this process thermodynamically favorable, because low hydrogen partial pressure is necessary for the utilization of substrates like lactate or ethanol (Baffert et al 2019). In our experiments hydrogen concentration in medium without SO 4 2was substantial.…”

Section: Resultsmentioning

confidence: 75%

Direct Fermentative Hydrogen Production from Cellulose and Starch with Mesophilic Bacterial Consortia

Zagrodnik

Seifert

2020

Polish Journal of Microbiology

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Hydrogen produced from lignocellulose biomass is deemed as a promising fuel of the future. However, direct cellulose utilization remains an issue due to the low hydrogen yields. In this study, the long-term effect of inoculum (anaerobic sludge) heat pretreatment on hydrogen production from untreated cellulose and starch was evaluated during repeated batch processes. The inoculum pretreatment at 90°C was not sufficient to suppress H2 consuming bacteria, both for starch and cellulose. Although hydrogen was produced, it was rapidly utilized with simultaneous accumulation of acetic and propionic acid. The pretreatment at 100°C (20 min) resulted in the successful enrichment of hydrogen producers on starch. High production of hydrogen (1.2 l H2/lmedium) and H2 yield (1.7 mol H2/molhexose) were maintained for 130 days, with butyric (1.5 g/l) and acetic acid (0.65 g/l) as main byproducts. On the other hand, the process with cellulose showed lower hydrogen production (0.3 l H2/lmedium) with simultaneous high acetic acid (1.4 g/l) and ethanol (1.2 g/l) concentration. Elimination of sulfates from the medium led to the efficient production of hydrogen in the initial cycles – 0.97 mol H2/molhexose (5.93 mmol H2/gcellulose). However, the effectiveness of pretreatment was only temporary for cellulose, because propionic acid accumulation (1.5 g/l) was observed after 25 days, which resulted in lower H2 production. The effective production of hydrogen from cellulose was also maintained for 40 days in a repeated fed-batch process (0.63 mol H2/molhexose).

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“…Besides, ten genera belonging to the phylum Firmicutes and thirteen genera belonging to the phylum Proteobacteria were found to be higher during the pregnancy period compared with the postnatal period. Desulfovibrio belongs to the phylum Proteobacteria and is a sulfate-reducing bacteria, which utilizes H 2 and sulfate to produce H 2 S [19]. Desulfovibrio, which belong to the phylum Proteobacteria, and Turicibacter, which belong to the phylum Firmicutes are often correlated with inflammation [20].…”

Section: Discussionmentioning

confidence: 99%

The Dynamic Changes of Gut Microbiota during the Perinatal Period in Sows

Lan

Chen

Lan

et al. 2020

Animals

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The gut microbiota in sows is important for the health of the host, and potential benefits may also be transferred to piglets during pregnancy. Therefore, systematic studies investigating the changes in the gut microbiota of sows are needed to elucidate the microbial compositions and functions. This study was conducted at 12 time points to investigate the temporal variations in gut microbiota on Days 27, 46, 64, 81, 100, and 113 during gestation (G) and Days 3, 5, 7, 10, 14, and 21 during lactation (L). Results suggested that the gut microbiota changed across the perinatal period with microbial function and abundance varying between the prenatal and postnatal periods. The alpha diversity was higher in the postnatal period than in the prenatal period. Thirty-eight genera were distributed between the two periods with Methanobrevibacter, Desulfovibrio, Akkermansia, and Turicibacter being enriched in the prenatal period while Eubacterium, Actinobacillus, Paludibacter, Butyricimonas, Megasphaera, Succiniclasticum, Acidaminococcus, and Rummeliibacillus were enriched in the postnatal period. Analysis done at the different time points of the prenatal period suggested that Days 27 and 113 had more microbial biomarkers than other days. Bacteroidales, Bacteroidia, and Prevotella were enriched on the 27th day, while bacteria belonging to the Clostridium and Ruminococcaceae were enriched on the 113th day. On the other hand, Clostridiales, Ruminococcaceae, Clostridia, and unclassified Christensenellaceae were enriched three days after delivery. Predicted microbial KO functions were also more enriched on Day 27 of the gestation period and Day 3 of the lactation period. Random forest, a machine learning method, was used to identify the top five important genera of Megasphaera, Stenotrophomonas, Phyllobacterium, Catenibacterium, and Turicibacter, while the most important function was arginine and proline metabolism. These systematic results provide important information for the gut microbiota of sows.

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Hydrogenases and H2 metabolism in sulfate-reducing bacteria of the Desulfovibrio genus

Cited by 39 publications

References 189 publications

HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases

HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases

Direct Fermentative Hydrogen Production from Cellulose and Starch with Mesophilic Bacterial Consortia

The Dynamic Changes of Gut Microbiota during the Perinatal Period in Sows

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Hydrogenases and H2 metabolism in sulfate-reducing bacteria of the Desulfovibrio genus

Cited by 39 publications

References 189 publications

HarnessingEscherichia colifor bio-based production of formate under pressurized H2and CO2gases

HarnessingEscherichia colifor bio-based production of formate under pressurized H2and CO2gases

Direct Fermentative Hydrogen Production from Cellulose and Starch with Mesophilic Bacterial Consortia

The Dynamic Changes of Gut Microbiota during the Perinatal Period in Sows

Contact Info

Product

Resources

About

HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases

HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases