An extract of cultured A431 cells contains major tissue antigens of autoimmune bullous diseases

Abstract

This finding suggests that culture extracts of A431 cells provide an effective substrate for the diagnosis and differential diagnosis of major AIBDs.

Pemphigus vulgaris presenting in a radiation portal

“…Immunoblot against the plakin family members, BP230, envoplakin 210, and periplakin 190, was negative as compared with a control patient with paraneoplastic pemphigus. 9 Immunomapping was performed on skin from within the radiation site and from nonirradiated skin using noncross-reactive monoclonal antibodies directed against Dsg1 and Dsg3 (R and D Systems Inc, Minneapolis, Minn). Staining for Dsg3 was seen in the basal and immediately suprabasal layer in both the irradiated (Fig 3, A) and nonirradiated skin.…”

Comparative analysis of methods for detection of anti-laminin 5 autoantibodies in patients with anti-epiligrin cicatricial pemphigoid

“…5,12 A-431 cell extracts were prepared by treating cell monolayers with Tris-buffered saline containing 1.5% SDS, 5% b-mercaptoethanol, 2 mM PMSF, 2 mM CaCl 2 , and 5 mM each of antipain, chymostatin, and pepstatin A. 13 HaCat cells were grown to near confluency, then the culture medium was removed, and the attached cells were lysed with 20 mM NH 4 OH. Residual HaCat cell proteins were solubilized in Laemmli sample buffer to yield HaCat cell extract.…”

Spectraplakin family proteins – cytoskeletal crosslinkers with versatile roles

“…Additional BPAG1e isoforms, including BPAG1eA, BPAG1eS and BPAG1eB, are found in muscle (Okumura et al, 2002). There are two BPAG1n isoforms, which are derived from the use of distinct neuronal transcriptional start sites that produce different short peptides preceding the F-actin-binding domain (Bernier et al, 1996;Lee, 2000;Yang et al, 1999). Furthermore, there is also neuronal BPAG1n3, whose transcription start site begins within the F-actin-binding domain (Liu et al, 2003).…”