An extract from <i>Spirulina platensis</i> is a selective inhibitor of herpes simplex virus type 1 penetration into HeLa cells

Hayashi, Kyoko; Hayashi, Toshimitsu; Morita, Naokata; Kojima, Ichiro

doi:10.1002/ptr.2650070118

Cited by 76 publications

(45 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although many health benefits of Spirulina have been claimed by algal manufacturers, scientific evidence is scarce. Although water soluble polysaccharides of Spirulina were previously found to inhibit the growth of HSV-1 [19][20][21] , there has been no report on anti HSV-1 activity of lipid extract from Spirulina. This work suggests that SQDG of Spirulina may help prevent viral infection in Vero cells.…”

Section: Resultsmentioning

confidence: 99%

“…Cyanobacterium, Spirulina platensis, is a rich source of valuable compounds such as the antioxidant phycocyanin and polyunsaturated fatty acids (linoleic acid and gamma-linolenic acid) which have an important role in human metabolic pathways, particularly as a precursor of one kind of prostaglandin 17,18 . Hayashi et al 19 were the first to report the anti HSV-1 activity of aqueous extracts from S. platensis. A water soluble fraction containing a sulphated polysaccharide called calcium spirulan isolated from S. platensis was found to inhibit a number of membrane viruses including HSV-1 [19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

“…Hayashi et al 19 were the first to report the anti HSV-1 activity of aqueous extracts from S. platensis. A water soluble fraction containing a sulphated polysaccharide called calcium spirulan isolated from S. platensis was found to inhibit a number of membrane viruses including HSV-1 [19][20][21] . Our preliminary study revealed that both water soluble and non-polar extracts of S. platensis exhibited antiviral activity on HSV-1 22 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Untitled

Chirasuwan¹,

Chaiklahan²,

Kittakoop³

et al. 2009

ScienceAsia

View full text Add to dashboard Cite

Herpes simplex virus type 1 (HSV-1) causes a variety of infections in humans and can be found in many countries worldwide. Although the antiherpes drug, acyclovir, has been used very successfully, an acyclovir-resistant strain of HSV has recently been found in patients and drug toxicity has been reported with increasing use of it. As a potential alternative, it has been found that both a water soluble and a lipid extract of the microalga Spirulina platensis exhibit antiviral activity (HSV-1) in Vero cells. In this study, bioassay guided fractionation of a lipid extract from S. platensis led to the identification of sulphoquinovosyl diacylglycerol as the active antiviral agent. The chemical structure of this compound was confirmed by NMR and LC-mass spectroscopic analyses. Results from the study showed that the compound exhibited a remarkable activity against HSV-1 with an IC50 value of 6.8 µg/ml, which was comparable to that of acyclovir (1.5 µg/ml).

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Chirasuwan¹,

Chaiklahan²,

Kittakoop³

et al. 2009

ScienceAsia

View full text Add to dashboard Cite

show abstract

“…'inhibition de ! 'adsorption du virus (Hayashi et al, 1993). Mais, les principes actifs presents dans les extraits aqueux de cyanobacteries ne sont pas encore identifies avec certitude.…”

Section: -Limites Des Modelcs Proposesunclassified

Les activités antivirales des polysaccharides sulfatés

Filali-Mouhim¹,

Hours²

1995

Acta Botanica Gallica

View full text Add to dashboard Cite

Resume.-Parmi las activites caracteristiques des polysaccharides at oligosaccharides sulfates, l'activite anti-virale est actuellement intensement exploree. Malgre las difficunes d'utilisation directe de cas molecules au niveau therapeutique, l'etude des mecanismes d'interactions des polysaccharides sulfates avec las virus at las cellules permet de concevoir at de synthetiser de nouvelles classes d'inhib~eurs. Ainsi, Ia recherche de nouveaux polysaccharides sulfates chez las macroalgues, las microalgues et les cyanobacteries est un objectif important pour l'avenir.Summary.-Sulfated oligo and polysaccharides have proved multiples activities and among them the antiviral activity is yet intensively examinated. In sp~e of the su~ated polysaccharides have no immediate application, the comprehension of the mechanisms of action is a main strategy for the construction of specific inhibitors of the enveloped virus. The research of new sulfated polysaccharides in macroalgae, microalgae and cyanobacteria is a challenge for the future.

show abstract

“…Na-SP and Ca-SP were isolated and purified from the blue-green alga Spirulina platensis as described previously. 10,21,22) Heparin derived from porcine intestine (M r ca. 10000) was purchased from Sigma (St Louis, MA, U.S.A.).…”

mentioning

confidence: 99%

Inhibition of Cultured Bovine Aortic Smooth Muscle Cell Proliferation by Colominic Acid

Yamamoto

Yamaguchi²,

Hayashi

et al. 2005

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Colominic acid (CA) is an a2,8-linked polymer of sialic acid, originally isolated from capsular Escherichia coli K1. 2) have several biological activities including antiviral activities, [3][4][5] inhibition of the cytotoxic action of bee and snake venom, 6) inhibition of P-selectin-dependent macrophage infiltration in the glomeruli in experimental rat crescentic glomerulonephritis 7) and inhibition of fertilization of mouse gametes. 8)Atherosclerosis is the principal vascular lesion in the pathogenesis of myocardial and cerebral infarction, which is initiated by vascular endothelial cell damage followed by an intimal hyperplasia of vascular smooth muscle cells. 9) Although the pathogenesis of atherosclerosis is not simple, inhibition of arterial smooth muscle cell proliferation is one of the effective strategies to prevent atherosclerosis.We have investigated the effects of sodium spirulan (Na-SP), a sulfated polysaccharide isolated from the blue-green alga Spirulina platensis 10) on vascular endothelial and smooth muscle cell functions, and found that the polysaccharide potently inhibits the proliferation of vascular smooth muscle cells.11) In vascular endothelial cells, Na-SP elevates the fibrinolytic activity of vascular endothelial cells through inhibition of the secretion of plasminogen activator inhibitor type 1.12) In addition, Na-SP promotes the secretion of anticoagulant proteoglycans 13) such as perlecan and biglycan, 14) as well as exhibiting an antithrombin activity through activation of heparin cofactor II. 15,16) We hypothesized that CA or SCA may inhibits the proliferation of vascular smooth muscle cell proliferation, as does natural or semi-synthetic polysaccharides, including Na-SP, heparin, 17) pentosan polysulfate 18) and fucoidan.19) The present study was undertaken to examine this hypothesis using a culture system of bovine aortic smooth muscle cells. It was found that both CA and SCA moderately inhibit the proliferation of the cells without nonspecific cell damage. MATERIALS AND METHODS MaterialsVascular smooth muscle cells obtained from bovine aorta were a gift from Professor Yasuo Suda (Graduate School of Science and Engineering, Kagoshima University, Kagoshima, Japan). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum were from Nissui Pharmaceutical (Tokyo, Japan) and MP Biomedicals (Irvine, CA, U.S.A.), respectively. Tissue culture plates and dishes were obtained from Iwaki (Chiba, Japan). H]thymidine (3.3 TBq/mmol) was from Parkin Elmer Life Sciences (Boston, MA, U.S.A.). Lactate dehydrogenase kit was from Wako Pure Chemical (Osaka, Japan). CA 20) (M r ca. 17000) and SCA 2) (M r ca. 22000) containing sulfur of 13.4% were isolated from capsular Escherichia coli K1 as sodium salt of an a2,8-linked homopolymer of N-acetylneuraminic acid with or without sulfation. Na-SP and Ca-SP were isolated and purified from the blue-green alga Spirulina platensis as described previously. 10,21,22) Heparin derived from porcine intestine (M r ca. 10000) was purchased from Sigma (St Louis...

show abstract

An extract from Spirulina platensis is a selective inhibitor of herpes simplex virus type 1 penetration into HeLa cells

Cited by 76 publications

References 12 publications

Untitled

Untitled

Les activités antivirales des polysaccharides sulfatés

Inhibition of Cultured Bovine Aortic Smooth Muscle Cell Proliferation by Colominic Acid

Contact Info

Product

Resources

About