2020
DOI: 10.1093/neuonc/noaa254
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An extracellular vesicle-related gene expression signature identifies high-risk patients in medulloblastoma

Abstract: Background Medulloblastoma (MB) is a malignant brain tumor in childhood. It comprises four subgroups with different clinical behaviors. The aim of this study was to characterize the transcriptomic landscape of MB, both at the level of individual tumors as well as in large patient cohorts. Methods We used a combination of single-cell transcriptomics, cell culture models and biophysical methods such as nanoparticle tracking ana… Show more

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Cited by 8 publications
(6 citation statements)
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“…However, a few studies imply that metabolic patterns might not be consistent across MB groups [ 15 , 16 ]. This is also exemplified in our previous work showing extensive metabolic reprogramming in the tumour of a mouse model for SHH MB, which affected lipid metabolism, nucleotide metabolism, and oxidative phosphorylation (OXPHOS) [ 17 ]. To answer whether specific alterations in metabolic dependencies could potentially serve as future therapeutic targets [ 18 ], detailed knowledge about the extent of metabolic diversity in MB and underlying molecular mechanisms is needed.…”
Section: Introductionmentioning
confidence: 69%
See 1 more Smart Citation
“…However, a few studies imply that metabolic patterns might not be consistent across MB groups [ 15 , 16 ]. This is also exemplified in our previous work showing extensive metabolic reprogramming in the tumour of a mouse model for SHH MB, which affected lipid metabolism, nucleotide metabolism, and oxidative phosphorylation (OXPHOS) [ 17 ]. To answer whether specific alterations in metabolic dependencies could potentially serve as future therapeutic targets [ 18 ], detailed knowledge about the extent of metabolic diversity in MB and underlying molecular mechanisms is needed.…”
Section: Introductionmentioning
confidence: 69%
“…Six human MB samples were dissociated and further processed into single-cell suspensions of vital tumour cells as described [ 17 ]. We applied chromium technology (10× Genomics) for single-cell capture, barcoding and cDNA amplification.…”
Section: Methodsmentioning
confidence: 99%
“…Although in molecular biology, such as DNA methylation, mRNA NanoString platform and single-cell sequencing, have resulted in the generation of large amounts of information that have been constructed multigene pro les [19][20][21][22][23][24]. Nonetheless, according to the NCCN Clinical Practice Guidelines in Oncology for Pediatric Central Nervous System Cancers, these should not be used as a rst-line molecular test.…”
Section: Discussionmentioning
confidence: 99%
“…Interestingly, single-cell profiling techniques, including single-cell sequencing and cytometry, provide an unprecedented opportunity to research the heterogeneity of the immune infiltration of tumors [ 146 ]. Currently, several studies have combined liquid biopsy and single-cell sequencing analyses, finding that they may be able to provide more information for a variety of cancer diagnoses and classifications [ 147 , 148 , 149 ]. However, thyroid cancer has not yet been investigated.…”
Section: Future Perspectivesmentioning
confidence: 99%