An extracellular laccase with potent dye decolorizing ability from white rot fungus Trametes sp. LAC-01

Ling, Zhuoren; Wang, Shanshan; Zhu, Mengjuan; Ning, Ying-Jie; Wang, Shou-Nan; Li, Bing; Yang, Aizhen; Zhang, Guoqing; Xiaomeng, Zhao

doi:10.1016/j.ijbiomac.2015.09.011

Cited by 30 publications

(16 citation statements)

References 58 publications

(95 reference statements)

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“…Immobilized laccase exhibited a strong decolorization capacity at short reaction times without mediator addition. Results significantly higher than those reported previously for Trypan Blue (Razak and Annuar, 2014), Bromothymol Blue (Ling et al, 2015) and Methyl Orange (Pardo et al, 2013) among others, showing the potential of immobilized biocatalyst developed.…”

Section: Decolorization Assayscontrasting

confidence: 52%

Development of strong enzymatic biocatalysts for dye decolorization

Britos

Trelles

2016

Biocatalysis and Agricultural Biotechnology

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Section: Decolorization Assayscontrasting

confidence: 52%

Development of strong enzymatic biocatalysts for dye decolorization

Britos

Trelles

2016

Biocatalysis and Agricultural Biotechnology

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“…A variety of reports highlighting the inducing effect of carbon sources in the decolourisation of dyes are available [10,19,24,25,42]. Figure 4 shows the results obtained for the expression of lcc2 using different carbon sources and the initial 9.5 and final 5.5 pHs obtained during the fermentation study.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, Ling et al [10] reported the decolourisation of synthetic dyes (Bromothymol Blue, Evans Blue, Fuchsin Basic, Malachite Green, Methylene Blue and Reactive Brilliant Blue R) using 0.5 U∙mL −1 purified Lcc from the WRF Trametes sp. LAC-01.…”

Section: Introductionmentioning

confidence: 99%

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

Ottoni

Simões

Fernandes

et al. 2016

IJERPH

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Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 U∙L−1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs.

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“…All enzymes decolorized 69% of RBY while less than 50% decolorization of RGY and RBR was detected in 7 days. Laccase of Trametes sp., Lac-01, had decolorizing ability on triarylmethane dyes, bromthymol blue and malachite green [23]. Laccase of Ganoderma sp.…”

Section: Dye Decolorization Capability Of Purified Mnp1 Mnp2 and Lac1mentioning

confidence: 99%

“…Many laccases have been reported from genus Trametes including T. versicolor [9], T. trogii [19], T. hirsuta [20], T. polyzona [21], T. maxima [22] and Trametes sp. [23]. These ligninolytic enzymes carry out many important functions involved in lignin synthesis and degradation of plant cell walls as well as morphogenesis of fungal fruiting body formation, pathogenicity, and stress responses [24][25][26][27].…”

Section: It Catalyzes H 2 O 2 -Dependent Oxidation Of Mn 2þmentioning

confidence: 99%

Two Manganese Peroxidases and a Laccase ofTrametes polyzonaKU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

et al. 2019

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Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from Trametes polyzona KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25 mg/L) in 10-30 min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were 50 C and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.

show abstract

An extracellular laccase with potent dye decolorizing ability from white rot fungus Trametes sp. LAC-01

Cited by 30 publications

References 58 publications

Development of strong enzymatic biocatalysts for dye decolorization

Development of strong enzymatic biocatalysts for dye decolorization

High Laccase Expression by Trametes versicolor in a Simulated Textile Effluent with Different Carbon Sources and PHs

Two Manganese Peroxidases and a Laccase ofTrametes polyzonaKU-RNW027 with Novel Properties for Dye and Pharmaceutical Product Degradation in Redox Mediator-Free System

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