An exploration of nucleic acid liquid biopsy using a glucose meter

Gu, Yu; Zhang, Tingting; Huang, Zhifeng; Hu, Songjie; Zhao, Wei; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1039/c8sc00627j

Cited by 57 publications

(27 citation statements)

References 32 publications

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“…All fluorescence kinetic curves were monitored with the Coyote Mini-8 portable realtime PCR machine (Beijing, China). Procedures of synthesis of TmINVeF conjugate, preparation of the TmINVeF:Bio-Q-MB conjugate, standard LAMP reaction and fluorescence CHA detection were similar as previously reported [15] and presented in supporting information.…”

Section: Chemicals and Materialsmentioning

confidence: 78%

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Guo

Dong

et al. 2020

Analytica Chimica Acta

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Section: Chemicals and Materialsmentioning

confidence: 78%

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Guo

Dong

et al. 2020

Analytica Chimica Acta

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“…NHS-PEG-maleimide has been the most extensively used crosslinker in antibody conjugation strategies in polymeric NPs for breast cancer treatment ( Table 1). SMCC is water-insoluble [83] but sulfo-SMCC is soluble in water due to a negatively charged sulfonate group on the NHS ring [84]. Therefore, using sulfo-SMCC as crosslinker, the free sulfhydryls of the antibody can be linked to the primary amines on the surface of the NPs via maleimide chemistry [85].…”

Section: Maleimide Chemistrymentioning

confidence: 99%

An Overview of Antibody Conjugated Polymeric Nanoparticles for Breast Cancer Therapy

et al. 2020

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Nanoparticles (NPs) are promising drug delivery systems (DDS) for identifying and treating cancer. Active targeting NPs can be generated by conjugation with ligands that bind overexpressed or mutant cell surface receptors on target cells that are poorly or not even expressed on normal cells. Receptor-mediated endocytosis of the NPs occurs and the drug is released inside the cell or in the surrounding tissue due to the bystander effect. Antibodies are the most frequently used ligands to actively target tumor cells. In this context, antibody-based therapies have been extensively used in HER2+ breast cancer. However, some patients inherently display resistance and in advanced stages, almost all eventually progress. Functionalized NPs through conjugation with antibodies appear to be a promising strategy to optimize targeted therapies due to properties related to biocompatibility, suitable delivery control and efficiency of functionalization. This review is focused on the different strategies to conjugate antibodies into polymeric NPs. Recent antibody conjugation approaches applied to the improvement of breast cancer therapy are highlighted in this review.

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“…The latter is far from the upper limit of the glucometer, where some PGM models lose their linear behavior [ 11 ]. Wider dynamic ranges have been described for nucleic acid quantification employing the PGM readout [ 27 , 28 ] but, considering the restricted detection range of the PGM (about one order of magnitude), the sensitivity of these genoassays tends to be lower, so a very small relative standard deviation is required for reliable quantification.…”

Section: Discussionmentioning

confidence: 99%

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Abardía-Serrano

Miranda‐Castro

de‐los‐Santos‐Álvarez

et al. 2020

Sensors

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A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL−1 pM−1) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.

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An exploration of nucleic acid liquid biopsy using a glucose meter

Abstract: A proof-of-concept strategy for a circulating miRNA assay using a personal glucose meter (PGM) was proposed.

Cited by 57 publications

References 32 publications

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

An Overview of Antibody Conjugated Polymeric Nanoparticles for Breast Cancer Therapy

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

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