“…Prior work has shown that poor immobilization results in dye leaching and consequently a drifting of the calibration of the probe, which leads to the gradual breakdown of its useful sensing ability [17]. Among several widely used immobilization methods are included absorption or entrapment [45][46][47], layer-by-layer (LbL) electrostatic self-assembly [48,49] and covalent binding [18,[50][51][52][53]. The first two approaches are generally easier and simpler but not suitable for this type of application as they suffer from numerous disadvantages such as cracking, dye leaching and inhomogeneity of the material (in case of absorption or entrapment in a sol-gel matrix) or irreversible damage at extreme pH values and issue related to effect of ionic strength (in case of electrostatic attachment).…”