An experimental ninhydrin design approach for the sensitive spectrofluorimetric assay of milnacipran in human urine and plasma

Mostafa, Islam M.; Derayea, Sayed M.; Nagy, Dalia M.; Omar, Mahmoud A.

doi:10.1016/j.saa.2018.07.040

Cited by 20 publications

(10 citation statements)

References 17 publications

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“…Most of the bioanalytical methods that quantify drugs in biological matrices are chromatographic techniques, which require a long analysis time, high-grade expensive solvents and sophisticated instrumentation [13][14][15]. These drawbacks can be resolved by the use of the sensitive, selective and fast spectrofluorometric technique [16][17][18]. Following oral administration of a single 100-mg dose of flibanserin, the the maximum concentration in plasma (C max ) was found to be 336 ng/mL.…”

Section: Resultsmentioning

confidence: 99%

Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples

Ahmed

Abdallah

2020

Molecules

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Green, economic and sensitive two spectrofluorometric methods were developed for the quantitation of flibanserin (FB) in different matrices, which are based on FB native fluorescence properties. The first technique depends on measuring the relative fluorescence intensity of FB directly at emission and excitation wavelengths(λem/λex) (371 nm/247 nm), while the second technique is a first derivative (D1) spectrofluorometric method, which depends on measuring the peak amplitudes at 351 nm. Linear regressions were observed in the range of 0.1–1.5 μg/mL for both methods. Moreover, both methods were efficiently extended to analyze FB in human urine, indicating the ultra-sensitivity of the methods, and linear regression was found within a range 0.05–0.7 μg/mL for both methods. Excellent selectivity of the proposed methods and good recoveries were obtained upon the analysis of FB in pharmaceutical dosage form and human urine samples without interference from matrix components with acceptable ranges, from 98.86 to 101.46% and from 98.08 to 102.37%, respectively. Greenness of the developed methods was assessed using the national environmental method index (NEMI) and Analytical Eco-scale and Green Analytical Procedure Index (GAPI). The three approaches confirmed that the developed methods are green, safe and environment-friendly.

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Section: Resultsmentioning

confidence: 99%

Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples

Ahmed

Abdallah

2020

Molecules

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“…Also, about 60% of MLC excreted in the urine without changing. 9 Approach application for MLC analysis in spiked (plasma/urine) was established and validated without any notable interference. The data in (Table 6) represent mean recovery of four spiked concentration was estimated from the corresponding regression equation shows the suitability of the approach as a preliminary test for MLC assay in spiked urine and plasma.…”

Section: Approach Application In Spiked Urine and Plasmamentioning

confidence: 99%

“…4 It acts by unique and equal inhibition of noradrenaline and serotonin neurotransmitters reuptake. 5 In the previous analytical literature, MLC was assayed by various techniques which include GC-MS, 6 liquid chromatography with uorescence or UV detection, 7,8 spectrouorimetry by derivatization with ninhydrin 9 or NBD-Cl, 10 UVspectrophotometric detection at 223 nm 11,12 and colorimetry using ninhydrin 13 or ion pair complex. 14 The approach target was simple development for accurate assay of MLC in the spiked biological uid, and tablets.…”

Section: Introductionmentioning

confidence: 99%

“…14 The approach target was simple development for accurate assay of MLC in the spiked biological uid, and tablets. The method provides advantages over the published method as the heating step is absent which make the method is more facile than the reported methods, 9,10 non-extractive, 13,14 low LOD and LOQ, 9,11,13,14 and nally implemented in non-sophisticated and easily available equipment.…”

Section: Introductionmentioning

confidence: 99%

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A new approach based on isoindole formation reaction for sensitive fluorimetric assay of milnacipran in tablets and biological fluids (plasma/urine)

2020

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“…[ 6 ] However, the developed method still suffered from low sensitivity (LOQ = 1.67 μg ml −1 ) and tedious procedures as it required heating at high temperature to 120°C using a glycerol bath. Spectrofluorimetric, [ 7–9 ] chromatographic [ 10–19 ] and capillary electrophoretic methods [ 20 ] were reported for assay of MCP. The reported spectrofluorimetric methods [ 7,8 ] used an expensive fluorogenic reagent that has a high fluorescence background and required acidification with high concentrations of hydrochloric acid to decrease reagent interference.…”

Section: Introductionmentioning

confidence: 99%

Use of Hantzsch reaction for quantitation of milnacipran as a magic treatment for fibromyalgia syndrome in human plasma and urine

et al. 2020

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A new fluorimetric procedure is described for analysis of milnacipran in its bulk, tablet dosage forms, as well as in biological human samples such as plasma and urine. The suggested method relies on the construction of a derivative with strong fluorescence called dihydropyridine derivative. This derivative resulted from the interaction of the primary amino group in the studied drug and acetylacetone/formaldehyde in McIlvaine buffer (pH 5). The fluorescent dihydropyridine derivative was measured at 470 nm. Influences of experimental variables namely pH, reagent concentration and temperature were examined and optimized. The calibration curve showed linearity over the range of 0.15–1.25 μg ml−1 of milnacipran with an R2 value of 0.9998. The detection limit was 0.02 μg ml−1 and the determination limit was 0.07 μg ml−1. The developed procedure was successfully used in the assay of the studied drug in Avermilan® tablets with excellent selectivity. In addition, the reaction was applied to estimate the drug in spiked human plasma and urine with mean percentage recoveries of 100.04 ± 1.61 and 99.78 ± 0.81% for urine and plasma, respectively.

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An experimental ninhydrin design approach for the sensitive spectrofluorimetric assay of milnacipran in human urine and plasma

Cited by 20 publications

References 17 publications

Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples

Development and Greenness Evaluation of Spectrofluorometric Methods for Flibanserin Determination in Dosage Form and Human Urine Samples

A new approach based on isoindole formation reaction for sensitive fluorimetric assay of milnacipran in tablets and biological fluids (plasma/urine)

Use of Hantzsch reaction for quantitation of milnacipran as a magic treatment for fibromyalgia syndrome in human plasma and urine

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