An exon skipping in a <i>SEPALLATA‐Like</i> gene is associated with perturbed floral and fruits development in cucumber

Wang, Xin; Gao, Dongling; Sun, Junling; Liu, Min; Lun, Yaoyao; Zheng, Jianshu; Wang, Shenhao; Cui, Qingzhi; Wang, Xiaofeng; Huang, Sanwen

doi:10.1111/jipb.12472

Cited by 20 publications

(14 citation statements)

References 27 publications

(33 reference statements)

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“…Cucumber (Cucumis sativus L.) is a major vegetable crop cultivated worldwide, and is also a model system for flower development studies owing to its diversity of floral sex types [38][39][40]. Our previous study had identified 43 MADS-box family genes in cucumber, and some of them may function in determining the flower organ identity and floral transition [1].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

Zhou

Song

et al. 2019

Biotechnology & Biotechnological Equipment

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

Zhou

Song

et al. 2019

Biotechnology & Biotechnological Equipment

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“…Previous study revealed the expression pattern of 43 MADS-box genes in cucumber (Hu and Liu, 2012). CUM1, CUM10, CUM26, CsAP3, and CsSEP2 are currently known cucumber MADS-box genes that play essential roles in flower and fruit development (Kater et al, 1998;Kater et al, 2001;Sun et al, 2016;Wang et al, 2016), whereas there are still a considerable portion of MADS-box genes need to be functionally characterized. In this study, we identified the CsSHP gene in cucumber and revealed the roles in fruit maturation and floral organ determination.…”

Section: Discussionmentioning

confidence: 99%

The MADS-Box Gene CsSHP Participates in Fruit Maturation and Floral Organ Development in Cucumber

et al. 2020

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Cucumber is an important vegetable crop bearing fleshy pepo fruit harvested immature. Fruits left unpicked in time during summer production, as well as unfavorable environmental conditions during post-harvest shelf, will cause cucumber fruits to turn yellow and ripen, and thus impair the market value. Identification of maturity-related genes is of great agricultural and economic importance for cucumber production. Here, we isolated and characterized a MADS-box gene, Cucumis sativus SHATTERPROOF (CsSHP) in cucumber. Expression analysis indicated that CsSHP was specifically enriched in reproductive organs including stamens and carpels. Ectopic expression of CsSHP was unable to rescue the indehiscence silique phenotype of shp1 shp2 mutant plant in Arabidopsis. Instead, overexpression of CsSHP resulted in early flowering, precocious phenotypes, and capelloid organs in wild-type Arabidopsis. Biochemical analysis indicated that CsSHP directly interacted with cucumber SEPALLATA (SEP) proteins. CsSHP expression increased significantly during the yellowing stage of cucumber ripening, and was induced by exogenous application of abscisic acid (ABA). Therefore, CsSHP may participate in fruit maturation through the ABA pathway and floral organ specification via interaction with CsSEPs to form protein complex in cucumber.

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“…Cucumber is one of the economically-important vegetable crops cultivated worldwide, and ABCDE model genes may play very important roles in flower development. However, only a number of floral homeotic genes from cucumber have been investigated in detail [28,31,37,38]. In this study, an AG homologous gene, CsMADS24, was isolated from cucumber developing flowers.…”

Section: Discussionmentioning

confidence: 99%

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

Zhou

et al. 2019

ARCH BIOL SCI BELGRA

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The floral homeotic C-function MADS gene AGAMOUS (AG) in Arabidopsis plays crucial roles in specifying stamen and carpel identities as well as determining floral meristem. However, there have been only a few studies of floral homeotic C-function genes in cucumber thus far. In the present study, CsMADS24, a putative AG ortholog from cucumber, was isolated and characterized. Sequence analysis and protein sequence alignment revealed that the deduced CsMADS24 protein contained the typical MIKC structure and the N-terminal extension, as well as two highly conserved AG motifs (I and II). Phylogenetic analysis showed that CsMADS24 fell into the clade of core eudicots, while being distant from the AG orthologs of basal eudicots, monocots and gymnosperms. Expression analysis by RT-PCR showed that CsMADS24 was exclusively expressed in female flower buds. In situ hybridization revealed that CsMADS24 expression was only detected in the carpels. Functional analyses indicated that the sepals were partly converted into carpelloid-like structures in 35S::35S::CsMADS24 transgenic plants. In addition, earlier flowering and delayed floral organ abscission during the development of siliques were also observed in transgenic Arabidopsis. Our findings demonstrate that the AG ortholog plays an exclusive role in carpel specification of cucumber, providing a basis for revealing the mechanisms of reproductive development in cucumber.

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An exon skipping in a SEPALLATA‐Like gene is associated with perturbed floral and fruits development in cucumber

Abstract: S (2016) An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber.

Cited by 20 publications

References 27 publications

Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

The MADS-Box Gene CsSHP Participates in Fruit Maturation and Floral Organ Development in Cucumber

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

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