Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

Zhou, Yong; Hu, Lifang; Song, Jianbo; Jiang, Lunwei; Liu, Shiqiang

doi:10.1080/13102818.2018.1534556

Cited by 13 publications

(16 citation statements)

References 56 publications

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“…RT-PCR was performed to examine the expression of CsMADS01 in different developmental stages of MF and FF using the reaction procedure previously described (Zhou et al, 2019a). The primer sequences are listed in Table S1.…”

Section: Gene Expression Analysismentioning

confidence: 99%

Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.)

Cheng

Jiang

et al. 2019

Not Bot Horti Agrobo

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SEPALLATA (SEP) genes encode the E class MADS-box transcription factors that play vital roles in various aspects of plant growth and development. In this study, we isolated a SEP-like gene from cucumber (Cucumis sativus L.), which was previously named as CsMADS01. CsMADS01 had a coding sequence (CDS) of 741 bp, and coded a deduced protein of 246 amino acid residues that was predicted to be located in the nucleus. The putative CsMADS01 protein was typically characterized by the MIKC-type MADS (including MADS, I, K, and C domains) and shared high homology with other SEP-like proteins. Phylogenetic analysis of CsMADS01 and SEP proteins from other plants revealed that CsMADS01 was a member of the SEP1/2 clade of SEP proteins and was the most closely related to ZjMADS48 from Chinese jujube. Promoter analysis showed that several cis-elements related to stress response and hormones were present in the promoter region of CsMADS01. Expression analysis indicated that CsMADS01 was highly expressed during the development of male and female flowers, and the expression increased gradually along with fruit development. Ectopic expression of CsMADS01 in Arabidopsis resulted in a phyllody-like phenotype and the transgenic plants never flowered. These results suggest that CsMADS01 plays an important role in the growth and development of cucumber. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Section: Gene Expression Analysismentioning

confidence: 99%

Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.)

Cheng

Jiang

et al. 2019

Not Bot Horti Agrobo

Self Cite

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“…The closest references were retrieved from NCBI GenBank and aligned with CLUSTAL W with all parameters set at their default values. Phylogenetic trees were then generated by a neighbour-joining algorithm [20] and Maximum Composite Likelihood model of base substitution as implemented in MEGA7.0.14 [21]. The trees were validated using bootstrap analysis performed with 1000 replicates.…”

Section: Identification Of Dmp-degrading Bacteriamentioning

confidence: 99%

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

Ji-xian

Wang

et al. 2019

Biotechnology & Biotechnological Equipment

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“…Moreover, the MIKC-type proteins own an extremely conserved MADS domain and three moderately conserved domains (I, K and C) [ 6 ]. The various members of the MADS-box gene family have been known to participate predominantly in developmental processes, such as plant vegetative reproduction [ 7 , 8 ], flower organ formation [ 9 , 10 , 11 ], male and female gametophyte development [ 12 ] and fruit development [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies showed that the triple mutants SEP1/2/3 in Arabidopsis caused all flower organs to turn into sepals. Additionally, the SEP gene could shorten the vegetative growth period and promote the early flowering of plants [ 11 , 18 , 20 , 21 , 22 ]. Besides the genes in the ABCDE model, other MADS-box genes are also responsible for sepal development.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Expression Profile of Class E Genes Related to Sepal Development in Nelumbo nucifera

Liu

Zhang

et al. 2021

Plants

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The lotus (Nelumbo Adans.) is an important aquatic plant with ornamental, medicinal and edible values and cultural connotations. It has single-, semi-double-, double- and thousand-petalled types of flower shape and is an ideal material for developmental research of flower doubling. The lotus is a basal eudicot species without a morphological difference between the sepals and petals and occupies a critical phylogenetic position in flowering plants. In order to investigate the genetic relationship between the sepals and petals in the lotus, the class E genes which affect sepal formation were focused on and analyzed. Here, SEPALLATA 1(NnSEP1) and its homologous genes AGAMOUS-LIKE MADS-BOXAGL9 (NnAGL9) and MADS-BOX TRANSCRIPTION FACTOR 6-like (NnMADS6-like) of the class E gene family were isolated from the flower buds of the Asian lotus (Nelumbo nucifera Gaertn.). The protein structure, subcellular localization and expression patterns of these three genes were investigated. All three genes were verified to locate in the nucleus and had typical MADS-box characteristics. NnSEP1 and NnMADS6-like were specifically expressed in the sepals, while NnAGL9 was highly expressed in the petals, suggesting that different developmental mechanisms exist in the formation of the sepals and petals in the lotus. The significant functional differences between NnSEP1, NnMADS6-like and NnAGL9 were also confirmed by a yeast two-hybrid assay. These results expand our knowledge on the class E gene family in sepal formation and will benefit fundamental research on the development of floral organs in Nelumbo.

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Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

Abstract: View related articles View Crossmark data Citing articles: 2 View citing articles Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

Cited by 13 publications

References 56 publications

Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.)

Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.)

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

Molecular Cloning and Expression Profile of Class E Genes Related to Sepal Development in Nelumbo nucifera

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