An Examination of Cellular Organization Within the Stratum Corneum by A Silver Staining Method

Mackenzie, Ian C.; Linder, J. E.

doi:10.1111/1523-1747.ep12676509

Cited by 47 publications

(13 citation statements)

References 9 publications

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“…Further examples of a columnar organization ( Fig. i) have been demonstrated in the epidermis ofthe hairless (hr/hr) mouse (Karatschai et al^ 1971), frog (Komnick & Stockum, 1970), chicken (Mackenzie, 1975a), hamster (Mackenzie, Zimmerman & Wheelock, 1975), and monkey (Menton & Eisen, 1971;Mackenzie & Linder, 1973;Mackenzie, Zimmerman & Peterson, 1981). The timings ofthe first appearance ofthe columnar structure in neonatal rat epidermis were followed by Mackenzie & Zimmerman (1981).…”

Section: Morphological Units In Epidermismentioning

confidence: 91%

Proliferative units in stratified squamous epithelium

Hume

Potten

1983

Clin Exp Dermatol

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Section: Morphological Units In Epidermismentioning

confidence: 91%

Proliferative units in stratified squamous epithelium

Hume

Potten

1983

Clin Exp Dermatol

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“…The growth of cultured epithelial cells grown on collagen more closely resembles the regular, hexagonal, columnar structure typical of epidermis [55,56]. Without air-interface growth, the submerged cultured cells do not produce the membrane-coating granules of in-vivo epidermis [35].…”

Section: Introductionmentioning

confidence: 91%

Cultured human keratinocytes for optical transmission measurement

Schaaf¹,

Eurell²,

Johnson³

2010

Journal of Biophotonics

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The challenges of measuring optical properties of human tissues include the thickness of the sample, homogenization, or crystallization from freezing of the tissue. This investigation demonstrates a method to avoid these problems by growing optically thin samples of human keratinocytes as a substitute for ex vivo epidermis samples. Several methods of growth were investigated. Resulting samples were measured on a spectrophotometer for transmission between 300 nm and 2600 nm. The efficacy of the cell growth was confirmed with histological examination of several cultured keratinocyte samples. Limitations were the requirement to measure samples immediately after removal from the incubation environment, and the absence of the irregular structures of normal skin such as hair and glands.

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“…They occupy an area of 700-1200 μm 2 ; thus, there are approximately 10 5 cells per cm 2 . They form a closely packed array of interdigitated cells (which facilitates the formation of cohesive laminae) which are the cells stacked in vertical columns (MacKensie and Linder 1973). Each cell is contained within a mainly proteinaceous envelope rather than the conventional lipid bilayer cell membrane.…”

Section: The Stratum Corneummentioning

confidence: 99%