2010
DOI: 10.1002/jbio.200910067
|View full text |Cite
|
Sign up to set email alerts
|

Cultured human keratinocytes for optical transmission measurement

Abstract: The challenges of measuring optical properties of human tissues include the thickness of the sample, homogenization, or crystallization from freezing of the tissue. This investigation demonstrates a method to avoid these problems by growing optically thin samples of human keratinocytes as a substitute for ex vivo epidermis samples. Several methods of growth were investigated. Resulting samples were measured on a spectrophotometer for transmission between 300 nm and 2600 nm. The efficacy of the cell growth was … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2010
2010
2019
2019

Publication Types

Select...
2
2

Relationship

1
3

Authors

Journals

citations
Cited by 4 publications
(2 citation statements)
references
References 63 publications
0
2
0
Order By: Relevance
“…However, the image-derived radioactivity concentration per volume or thickness is not easily obtained. It will be possible to calculate the value using data on the thickness distribution of tissues, which can be estimated from the radiation and optical 24,25 attenuation coefficient map. The radiation attenuation coefficient can be obtained from the uptake value difference in the autoradiographic images between no tissue (background) and no uptake tissue (at time zero) similar to the attenuation correction using transmission data in PET 26 (see Supplementary Fig.…”
Section: Discussionmentioning
confidence: 99%
“…However, the image-derived radioactivity concentration per volume or thickness is not easily obtained. It will be possible to calculate the value using data on the thickness distribution of tissues, which can be estimated from the radiation and optical 24,25 attenuation coefficient map. The radiation attenuation coefficient can be obtained from the uptake value difference in the autoradiographic images between no tissue (background) and no uptake tissue (at time zero) similar to the attenuation correction using transmission data in PET 26 (see Supplementary Fig.…”
Section: Discussionmentioning
confidence: 99%
“…Last, the dermis has been reported to be from 981 m to 4000 m thick. 42 This places the optical penetration depth of 2.0-m laser light within the dermis if absorption is dominant in skin. If skin is a turbid medium with scatter dominant, then the penetration depth is deeper into or beyond the dermis.…”
Section: Introductionmentioning
confidence: 98%