An Evaluation of the Polymerase Chain Reaction Amplicor Chlamydia trachomatis in Male Urine and Female Urogenital Specimens

Bianchi, A.; Scieux, Catherine; Brunat, N; Vexiau, D.; Kermanac'h, M.; P, Pezin; Janier, M.; Morel, Patrice; Ph, Lagrange

doi:10.1097/00007435-199407000-00003

Cited by 65 publications

(26 citation statements)

References 15 publications

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“…It is important to stress that each infected cell contains 50 IFU, on average. This makes the PCR method much more sensitive than other methods like culture, ELISA, or direct immunofluorescence [10].…”

Section: Methodsmentioning

confidence: 99%

Detection of Chronic Endometritis by Diagnostic Hysteroscopy in Asymptomatic Infertile Patients

Polisseni

Bambirra²,

Camargos³

2003

Gynecol Obstet Invest

100

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Chronic endometritis has been related to infertility and recurrent abortion. It is usually asymptomatic, and the diagnosis is rarely clinically suspected. We performed a prospective study to evaluate both the role of diagnostic hysteroscopy in the detection of chronic endometritis in infertile patients and Chlamydia trachomatis is a potential etiologic factor. Fifty consecutive patients who sought treatment for infertility in a tertiary academic hospital were submitted to diagnostic hysteroscopy and an endometrial biopsy for histopathological study and for diagnosis of C. trachomatis by polymerase chain reaction. The patients’ mean age was 33.7 ± (SD) 5.4 years, and the duration of the couples’ infertility ranged from 1 to 18 years. The overall prevalence of chronic endometritis was 12% (6 patients). Among all patients, no cases of chlamydial infection were detected by polymerase chain reaction. In the detection of chronic endometritis, with 95% confidence intervals, the hysteroscopy sensitivity was 16.7% (range 0.9–63,5%), the specificity was 93.2% (range 80.3–98.2%), the positive predictive value was 25% (range 1.3–78.1%), and the negative predictive value was 89.1% (range 65.6–95.9%). These data suggest that hysteroscopy is not useful in the screening for chronic endometritis in asymptomatic infertile women. Further studies are needed to establish the etiology of endometritis in infertile patients.

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Section: Methodsmentioning

confidence: 99%

Detection of Chronic Endometritis by Diagnostic Hysteroscopy in Asymptomatic Infertile Patients

Polisseni

Bambirra²,

Camargos³

2003

Gynecol Obstet Invest

100

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“…In mainland France, commercially available PCR methods have showed that the prevalence of such infection in symptomatic populations was 8–10.2% in women and 16.4% in men (1994–1998) 13 14. In the Caribbean region, the prevalence ranges from 5.4% to 21% in asymptomatic women from Curaçao, Barbados and Trinidad, to 55% in sexually transmitted infection clinic patients from Jamaica 15–19.…”

Section: Introductionmentioning

confidence: 99%

Serological reactivity and bacterial genotypes in Chlamydia trachomatis urogenital infections in Guadeloupe, French West Indies

Weill

Hello

Clerc

et al. 2009

Sexually Transmitted Infections

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“…Numerous evaluations have shown that amplified DNA techniques are markedly more sensitive than the antigen detection methods [3][4][5][6][7][8][9]. Furthermore they permit a noninvasive laboratory diagnosis in male and female patients, since DNA sequences may be detected from first void urine (FVU) samples, thus permitting an adequate therapy monitoring.…”

Section: Introductionmentioning

confidence: 99%

Low Correlation of Serology with Detection of Chlamydia trachomatis by Ligase Chain Reaction and Antigen EIA

Rabenau

Kohler²,

Peters³

et al. 2000

Infection

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The aim of the present study was to evaluate the diagnostic accuracy of serology by using new assays for the detection of genus and species-specific IgG, IgM, IgA and secretory IgA antibody in female sex workers. Cervical swabs and first void urine (FVU) from 314 female sex workers were submitted to nucleic acid amplification by ligase chain reaction (LCx, Abbott). Concomittantly, blood samples were tested for the presence of IgG, IgM and IgA antibodies using a genus-specific assay (rELISA, Medac) and species-specific test (SeroCT, Orgenics). Chlamydia trachomatis infection was detected in a total of 30 (9.6%) female sex workers by LCR. With rELISA, seroprevalences for IgG, IgM, and IgA antibody to Chlamydia were 88.9%, 19.1% and 62.7%, respectively. IgG and IgA antibody prevalences against C. trachomatis (SeroCT) were 65.0% and 23.9%, respectively. In comparison to the positive LCR results obtained from cervical swab and/or FVU, the sensitivity of rELISA for Chlamydia IgG, IgA and IgM detection was 93.3%, 83.3% and 16.7%, respectively. With SeroCT, the sensitivity for C. trachomatis-specific IgG and IgA detection was 86.7% and 33.3%, respectively. The specificities of both serologic tests in comparison to LCR were very low. In conclusion, the correlation of serology with active C. trachomatis infection of the lower genital tract is very low. According to our results, serologic testing for Chlamydia can exclude active infection of the lower genital tract with a high reliability (> or = 95%). However, detection of C. trachomatis can only be reliably achieved by nucleic acid amplification assays.

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An Evaluation of the Polymerase Chain Reaction Amplicor Chlamydia trachomatis in Male Urine and Female Urogenital Specimens

Cited by 65 publications

References 15 publications

Detection of Chronic Endometritis by Diagnostic Hysteroscopy in Asymptomatic Infertile Patients

Detection of Chronic Endometritis by Diagnostic Hysteroscopy in Asymptomatic Infertile Patients

Serological reactivity and bacterial genotypes in Chlamydia trachomatis urogenital infections in Guadeloupe, French West Indies

Low Correlation of Serology with Detection of Chlamydia trachomatis by Ligase Chain Reaction and Antigen EIA

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