An evaluation of the application of CHEMTAX to Antarctic coastal pigment data

Kozlowski, Wendy; Deutschman, Douglas H.; Garibotti, Irene A.; Trees, Charles C.; Vernet, María

doi:10.1016/j.dsr.2011.01.008

Cited by 84 publications

(88 citation statements)

References 54 publications

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“…SAZ Subantarctic Zone, PFZ Polar Frontal Zone, AZ Antarctic Zone communities are separated by the oceanic fronts in this area. The range of the Chl a concentrations measured in our study coincides with values generally found in Antarctic surface waters (Ishikawa et al 2002;Wright et al 2009;Kozlowski et al 2011). In the southern Indian Ocean, Chl a concentrations in late spring vary between 0.2 and 0.4 lg L -1 but in areas influenced by Southern Ocean waters, they can increase to 1.4 lg L -1 (Schlüter et al 2011).…”

Section: Structure Overviewsupporting

confidence: 91%

“…The CHEMTAX data cannot confirm the dominance of dinoflagellates, because most dinoflagellates are heterotrophic (Sherr and Sherr 2007) and thus do not have pigments. It is not uncommon for dinoflagellates to occur in Antarctic waters (Kozlowski et al 2011), but such a high contribution as we found was not observed so far. Dinoflagellates might be overrepresented in the sequence data, because of the high number of gene copies (also discussed later).…”

Section: Community Compositioncontrasting

confidence: 66%

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Protist community composition in the Pacific sector of the Southern Ocean during austral summer 2010

et al. 2013

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Knowledge about the protist diversity of the Pacific sector of the Southern Ocean is scarce. We tested the hypothesis that distinct protist community assemblages characterize large-scale water masses. Therefore, we determined the composition and biogeography of late summer protist assemblages along a transect from the coast of New Zealand to the eastern Ross Sea. We used state of the art molecular approaches, such as automated ribosomal intergenic spacer analysis and 454-pyrosequencing, combined with high-performance liquid chromatography pigment analysis to study the protist assemblage. We found distinct biogeographic patterns defined by the environmental conditions in the particular region. Different water masses harbored different microbial communities. In contrast to the Arctic Ocean, picoeukaryotes had minor importance throughout the investigated transect and showed very low contribution south of the Polar Front. Dinoflagellates, Syndiniales, and small stramenopiles were dominating the sequence assemblage in the Subantarctic Zone, whereas the relative abundance of diatoms increased southwards, in the Polar Frontal Zone and Antarctic Zone. South of the Polar Front, most sequences belonged to haptophytes. This study delivers a comprehensive and taxon detailed overview of the protist composition in the investigated area during the austral summer 2010.

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Section: Structure Overviewsupporting

confidence: 91%

Section: Community Compositioncontrasting

confidence: 66%

Protist community composition in the Pacific sector of the Southern Ocean during austral summer 2010

et al. 2013

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“…Because of the numerical dominance of small cells, the counting of 300 cells at one (high) magnification might lead to an underestimation of the contribution of larger cells that, although less abundant, are often the major contributors to biomass. Other studies comparing phytoplankton characterization by microscopy and chemotaxonomy in the region of WAP showed high qualitative agreement (Garibotti et al 2003a;Kozlowski et al 2011), but the correlation coefficient was usually higher for the intermediate and high biomass communities (diatoms) than for low biomass communities (prasinophytes and mixed flagellates). The differences may result from variations and modifications in the cell carbon calculation methods, which are still under debate.…”

Section: Discussionmentioning

confidence: 88%

Temporal and spatial variation of phytoplankton in Admiralty Bay, South Shetlands: the dynamics of summer blooms shown by pigment and light microscopy analysis

2015

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We conducted an integrated study of phytoplankton taxonomic composition, biomass and physicochemical properties of the water in Admiralty Bay, South Shetlands. The aim of the study was to provide detailed information on phytoplankton composition and diversity related to relevant environmental conditions. High-performance liquid chromatography pigment analysis and microscopy were performed in the summers of 2007 and 2009-2010. Results for 2007 showed a typical high-nutrient, low-chlorophyll system. Total carbon biomass and cell numbers were dominated by nanoflagellates, while diatoms made up 1.2-4.5 % of the total algal numbers and contributed a maximum of 23.4 % to the total cell carbon. A small algal bloom occurred in the center of the bay, with chlorophyll a values of *1.0 lg l -1 . The prevalent pigment was 19 0 -hexanoyloxyfucoxanthin, characteristic of the Prymnesiophyceae. In January 2010, the values of chlorophyll a (8-24 lg l -1 ) and cell carbon (150 lg l -1 ) were the highest ever recorded for Admiralty Bay. In general the algal populations were dominated numerically by nanoflagellates, but a bloom of diatoms (maximum 30 % of total cells and 50 % of total cell carbon) was observed. Diatoms were dominated by those of micro-size: Thalassiosira ritscheri and T. antarctica. The dominant pigment was fucoxanthin, mainly found in diatoms. The diatom bloom could be related to the southeast wind direction, stable water column conditions and an inflow of diatom-rich waters from the Bransfield Strait, while the input of high-turbidity, low-salinity water from melting glaciers and strong katabatic northwest winds could favor nanoflagellates.

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“…Seawater collected from Go-Flo bottles at different depths was filtered onto GF/F filters, then the filters were wrapped in foil and frozen at À 80°C for fluorometric phytoplankton chl-a analysis 42 ARTICLE or flash frozen in liquid nitrogen and stored at À 80°C for high-performance liquid chromatography analysis for phytoplankton accessory pigments/ phytoplankton composition 43 (mg pigment m À 3 ). The taxonomic composition of the phytoplankton assemblages was derived quantitatively from an analysis of high-performance liquid chromatography pigment data with CHEMTAX (V195) (refs 43,44) using initial pigment ratios previously derived from WAP phytoplankton 45 . For each vertical profile, we calculated the depth-integrated chl-a and accessory pigments (0-50 m; mg m À 2 ).…”

Section: Methodsmentioning

confidence: 99%

Winter and spring controls on the summer food web of the coastal West Antarctic Peninsula

Fraser²,

et al. 2014

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An evaluation of the application of CHEMTAX to Antarctic coastal pigment data

Cited by 84 publications

References 54 publications

Protist community composition in the Pacific sector of the Southern Ocean during austral summer 2010

Protist community composition in the Pacific sector of the Southern Ocean during austral summer 2010

Temporal and spatial variation of phytoplankton in Admiralty Bay, South Shetlands: the dynamics of summer blooms shown by pigment and light microscopy analysis

Winter and spring controls on the summer food web of the coastal West Antarctic Peninsula

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