An evaluation of the API ZYM system as a means of classifying spirochaetes associated with swine dysentery

Hunter, D; Wood, Thomas H.

doi:10.1136/vr.104.17.383

Cited by 55 publications

(33 citation statements)

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“…In the API ZYM system the Kla strain revealed the presence of an alpha galactosidase, an enzyme which was absent in strains that are known to cause dysentery (Hunter and Wood, 1979). In addition, the inability to produce indole (Kinyon and Harris, 1979) gives further evidence that strain Kla differs from T. hyodysenteriae.…”

Section: Discussionmentioning

confidence: 96%

Infectious Typhlitis in chickens caused by spirochetes

et al. 1986

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Section: Discussionmentioning

confidence: 96%

Infectious Typhlitis in chickens caused by spirochetes

et al. 1986

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“…However, the different species had to be grown in different media, which might have influenced the presence of inducible enzymes. There was, however, no indication of a medium effect when the results of the API ZYM Complete Research kit were compared with those of the API, ZYM or Rap ID-ANA systems using other culture media (Hunter & Wood, 1979;Syed et al, 1988;Fukumoto et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Comparison of peptidase, glycosidase and esterase activities of oral and non-oral Treponema species

Mikx

1991

Microbiology

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The enzyme profiles of 20 oral and non-oral Treponema strains were investigated using an API ZYM Complete Research kit. The test included 10 2-naphthyl derivatives of fatty acids, 20 p-nitrophenol derivatives of carbohydrates and 60 2-naphthylamide derivatives of amino acids and peptides. The oral Treponema species investigated were T. denticola, T. vincentii and T. pectinovorum. The non-oral species examined were T. phagedenis, T. hyodysenteriae and intestinal spirochaetes of human and chicken origin. Esterase activities on C5 to Clo fatty acids were common among different Treponema species. Glycosidase activities were infrequently observed in T. vincentii, T. pectinovorum and T. phagedenis Reiter strain. Arabinosidaw, lactosidase and xylssidase activity was observed in the T. hyodysenteriae strains but a-L-fucosidase activity was found only in T. denticola and T. phagedenis. More exo-and endo-peptidase activities were found in T. denticola than in other species. The enteropathogenic T. hyodysenteriae isolates had a very low proteolytic profile. Dipeptidyl prolyl amidase activity was observed in all species except in the T. phagedenis Reiter strain and the avian intestinal spirochaetes. The enzyme profiles did not discriminate between oral and non-oral Treponema species.

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“…Exhibits alkaline phosphatase, C4 esterase, C8 esterase lipase, phosphatase acid, P-galactosidase, a-glucosidase, and p-glucosidase activities (15).…”

Section: Discussionmentioning

confidence: 99%