An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa

Chisi, Songelwayo L.; Marageni, Yoanda; Naidoo, Prebashni; Zulu, Gloria; Akol, G.W.O.; Heerden, Henriëtte van

doi:10.4102/jsava.v88i0.1381

Cited by 32 publications

(43 citation statements)

References 13 publications

(28 reference statements)

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“…In the RBT test, any agglutination observed was considered as test positive. In the CFT test, if haemolysis was present, the sample was considered negative, and the absence of haemolysis as indicated by a red button in the centre of the well, was considered positive as described by Chisi et al [13]. …”

Section: Methodsmentioning

confidence: 99%

Serological prevalence and public health significance of brucellosis on a dairy farm in Namibia from 2011 to 2014

Madzingira

Sezuni

2017

BMC Res Notes

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ObjectiveThe main objective of this study was to determine the serological prevalence of brucellosis on a dairy farm with no past history of abortions, but where Brucella control measures including test and slaughter and vaccination of heifers at 4–8 months of age was practiced. Secondary data from 2011 to 2014 obtained from the Epidemiology Section of the Directorate of Veterinary Services was used.ResultsMandatory annual brucellosis testing results for mature dairy cows on a dairy farm for the period 2011–2014 were collated and analyzed. Results of a total of 6912 cows were analysed. The data comprised of the year of testing, number of cows tested for Brucella antibodies and the number of cows that tested positive. Serological testing was carried out using the Rose Bengal Test (RBT) as a screening test and the Complement Fixation Test as a confirmatory test for results that tested positive on the RBPT. Over the 4-year period, one dairy cow tested positive for Brucella antibodies in 2013 giving an apparent prevalence of 0.05% and an overall prevalence of 0.01%. When apparent prevalence was adjusted for RBPT test specificity and sensitivity of 71 and 78% respectively, true prevalence was determined to be zero.

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Section: Methodsmentioning

confidence: 99%

Serological prevalence and public health significance of brucellosis on a dairy farm in Namibia from 2011 to 2014

Madzingira

Sezuni

2017

BMC Res Notes

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“…Serological testing in cattle involves a combination of more than one serological test [ 10 ]. A combination of the Rose Bengal test (RBT) and indirect enzyme-linked immunosorbent assay (i-ELISA) is among the tests of choice due to its high sensitivity and specificity [ 11 , 12 ]. Although two serological tests are recommended for research and diagnosis of brucellosis, only RBT is widely used in most resource-poor countries [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and Associated Risk Factors of Bovine Brucellosis at the Wildlife-Livestock-Human Interface in Rwanda

et al. 2020

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Bovine brucellosis is endemic in Rwanda; however, little information is available on seroprevalence and risk factors. Therefore, a cross-sectional study was conducted among cattle farmed at the wildlife-livestock-human interface (n = 1691) in five districts and one peri-urban district (n = 216). Cattle were screened using the Rose Bengal test, then the results were confirmed by indirect enzyme-linked immunesorbent assay. Potential risk factors were determined with a questionnaire and analyzed for their association with seropositivity. In all districts, the animal and herd-level seroprevalence was 7.4% (141/1907) and 28.9% (61/212), respectively, 8.3% (141/1691) and 30.9% (61/198) at the interface, and 0.0% (0/216) in peri-urban areas. Among the potential risk factors, old age (≥5 years), cattle farmed close to wildlife, herds of cattle and small ruminants, history of abortions, and replacement animals were significantly associated with brucellosis (p < 0.05). Low awareness of zoonotic brucellosis, assisting calving without biosafety protection, drinking raw milk, and manual milking were each observed in more than 21.7% of cattle keepers whose herds were seropositive. This study confirmed brucellosis endemicity in cattle farmed close to wildlife in Rwanda, suggesting the need to focus control efforts in these areas. Educated farmers with a high awareness of zoonotic brucellosis had low bovine brucellosis seropositivity, which emphasizes the importance of education.

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“…This farmer’s cattle were tested in late October/early November 2016 using standard South African veterinary testing guidelines. 6 Sixty-eight cows were tested and 13 were found to be positive for B. melitensis , using the Rose Bengal test, complement fixation test and serum agglutination test, all of which are serology-based tests. The affected cattle were then isolated, sent to an abattoir and slaughtered as stipulated in the control programme of brucellosis in animals of South Africa – Animal Diseases Act (Act No.…”

Section: Management and Outcomesmentioning

confidence: 99%

“…9.1, 9.2, 11). 6 The farmer was instructed to send his milk to another local farm for pasteurisation until this farmer was able to pasteurise effectively on his own farm.…”

Section: Management and Outcomesmentioning

confidence: 99%

Brucellosis – laboratory workers’ nightmare come true: A case study

Skosana

Ismail²,

Mbelle

et al. 2020

African Journal of Laboratory Medicine

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Introduction: Brucella spp. are rarely encountered organisms in the medical microbiology laboratory and, when encountered, can cause concern in laboratory workers. Laboratory personnel may in fact develop serious disease as a result of this exposure. This case highlights shortcomings in recognition of Brucella spp. from a patient presenting atypically as well as the follow-up and management of an infected patient.Case presentation: The patient was an 8-year-old boy from a rural area of South Africa who presented to an academic hospital with a bladder mass and history of enuresis in September 2016. Brucella melitensis was isolated from a blood culture submitted to the laboratory. The child was subsequently treated for brucellosis in November 2016.Management and outcome: The source of infection in the patient was traced to consumption of unpasteurised milk from a local farmer. The patient was treated with doxycycline 100 mg twice daily and rifampicin 600 mg daily for 6 weeks and completed treatment, however he was not followed up at our hospital. The laboratory personnel, however, did not handle the specimen as a Biosafety Level 3 pathogen as this organism is not commonly encountered; they were provided with prophylaxis for brucellosis (rifampicin and doxycycline).Conclusion: Brucella spp. is a dangerous pathogen, easily capable of causing significant exposure in an unsuspecting and unprepared laboratory. The case discusses the management of brucellosis in the infected patient as well as the management of laboratory exposure to Brucella spp. Our case also describes the public health response to a case of brucellosis.

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An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa

Cited by 32 publications

References 13 publications

Serological prevalence and public health significance of brucellosis on a dairy farm in Namibia from 2011 to 2014

Serological prevalence and public health significance of brucellosis on a dairy farm in Namibia from 2011 to 2014

Seroprevalence and Associated Risk Factors of Bovine Brucellosis at the Wildlife-Livestock-Human Interface in Rwanda

Brucellosis – laboratory workers’ nightmare come true: A case study

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