ObjectiveThe main objective of this study was to determine the serological prevalence of brucellosis on a dairy farm with no past history of abortions, but where Brucella control measures including test and slaughter and vaccination of heifers at 4–8 months of age was practiced. Secondary data from 2011 to 2014 obtained from the Epidemiology Section of the Directorate of Veterinary Services was used.ResultsMandatory annual brucellosis testing results for mature dairy cows on a dairy farm for the period 2011–2014 were collated and analyzed. Results of a total of 6912 cows were analysed. The data comprised of the year of testing, number of cows tested for Brucella antibodies and the number of cows that tested positive. Serological testing was carried out using the Rose Bengal Test (RBT) as a screening test and the Complement Fixation Test as a confirmatory test for results that tested positive on the RBPT. Over the 4-year period, one dairy cow tested positive for Brucella antibodies in 2013 giving an apparent prevalence of 0.05% and an overall prevalence of 0.01%. When apparent prevalence was adjusted for RBPT test specificity and sensitivity of 71 and 78% respectively, true prevalence was determined to be zero.