An evaluation of pharmacological healing potentialities of Terminalia Arjuna against several ailments on experimental rat models with an in-silico approach

Tahsin, Md. Rafat; Sultana, Arifa; Khan, Muhammad Shah Mohtasim; Jahan, Ishrat; Mim, Sabiha Rahman; Tithi, Tanzia Islam; Ananta, Mokaddas Flora; Afrin, Sadia; Ali, Mehnaz; Hussain, M.; Chowdhury, Jakir Ahmed; Kabir, Shaila; Chowdhury, Abu Asad; Amran, Md. Shah; Aktar, Fahima

doi:10.1016/j.heliyon.2021.e08225

Cited by 5 publications

(5 citation statements)

References 65 publications

(117 reference statements)

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“…This study aims to compare the phytocompounds of G. procumbens against acarbose, which is an alpha-glucosidase and alpha-amylase inhibitor [ 23 ]. Docking of flavonoids and phenols as ligands with crystal targets alpha-glucosidase and alpha-amylase would define the binding affinity along with explaining the molecular pathway of inhibition by the extract of G. procumbens [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

Tahsin

Tithi

Mim

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Diabetes mellitus is one of the most notable health dilemmas. Analyzing plants for new antidiabetic remedies has become an impressive territory for life science researchers. Gynura procumbens has long been used to treat diabetes. Thus, we strived to ascertain the hypoglycemic potentiality of extract of leaves of G. procumbens by in vivo and in silico approaches. Methods. Fresh leaves of G. procumbens were collected and shade-dried to prepare ethanolic extracts to evaluate pharmacological parameters. Diabetes was induced in rats via injecting alloxan through the intraperitoneal route at a dose of 150 mg/kg body weight. Humalyzer 3000 was used to perform a biochemical assay of collected samples from rats. Anti-hyperglycemic activity study along with overdose toxicity test was performed. The pharmacological activity of this plant was also evaluated through a molecular docking study. This in silico study investigated the binding affinity of natural ligands from G. procumbens against glycoside hydrolase enzymes. Results. We detected a peak plasma concentration of G. procumbens at 3 hours 45 minutes that is roughly similar to the peak plasma concentration of metformin. Again, in OGTT and anti-hyperglycemic tests, it has been ascertained that both plant extract and metformin can exert significant P < 0.05 and highly significant P < 0.01 hypoglycemic activity in a dose-dependent manner. Metformin exhibited better therapeutic efficacy than that of plant extract, but it possessed null statistical significance. Also, our safety profile expressed that, similar to metformin, the plant extract can restore the disturbed pathological state in a dose-oriented approach with a wide safety margin. In silico study also validated the potentialities of natural constituents of G. procumbens. Conclusion. This study suggested that G. procumbens can be considered as potential antidiabetic plant. Robust and meticulous investigation regarding plant chemistry and pharmacology in the future may bring about a new dimension that will aid in discovering antidiabetic drugs from this plant in the diabetes management system.

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Section: Introductionmentioning

confidence: 99%

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

Tahsin

Tithi

Mim

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…A standard hepatoprotective drug named silymarin (Brand name: silybin) was obtained as a gift from Square Pharmaceuticals Ltd. Again, SGPT, SGOT, ALP, Creatinine, Total Cholesterol, HDL, LDL, Triglyceride were assessed by using blood serum analyzing kits, which were bought from Orbit Trade Ltd, and Gentech Ltd, Bangladesh. These parameters were analyzed by Humalyzer 3000, Germany [50,51]. LDH, CAT, SOD, and MDA were measured by maintaining the parameters provided by Jianchueng Biochemical Technological Institute, Nanjing [52].…”

Section: Drugs and Chemicalsmentioning

confidence: 99%

“…Thus, our sample size will be 8.97/0.9 = 9.97. As the sample size cannot be a fraction, ten rats were taken in each group [51].…”

Section: Andmentioning

confidence: 99%

“…3D coordinate files of the constituents were either downloaded from the 'PubChem' or the 'ChemSpider' databases or drawn manually on the 'Avogadro' software package [37,48]. The macromolecules TGF-β type I Receptor (PDB ID: 1VJY) and PPAR-α (PDB ID: 5HYK) were selected as the molecular targets for the docking studies, as these have been reported to be potent molecular mediators and regulators of hepatoprotective action [50,51,57,58]. The active sites of the macromolecules were deduced from literature, and galunisertib and bezafibrate were employed as control molecules, respectively [59][60][61][62].…”

Section: In Silico Molecular Docking Molecular Dynamics and Admet Stu...mentioning

confidence: 99%

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An in vivo and in silico evaluation of the hepatoprotective potential of Gynura procumbens: A promising agent for combating hepatotoxicity

Tithi,

Tahsin,

Anjum

et al. 2023

PLoS ONE

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Introduction The liver, the most important metabolic organ of the body, performs a wide variety of vital functions. Hepatic cell injury occurs by the activation of reactive oxygen species (ROS) that are generated by carbon tetrachloride (CCl4), xenobiotics, and other toxic substances through cytochrome P450-dependent steps resulting from the covalent bond formation with lipoproteins and nucleic acids. Observing the urgent state of hepatotoxic patients worldwide, different medicinal plants and their properties can be explored to combat such free radical damage to the liver. In vivo and in silico studies were designed and conducted to evaluate the antioxidant and hepatoprotective properties of Gynura procumbens in rats. Materials and methods Gynura procumbens leaves were collected and extracted using 70% ethanol. The required chemicals CCl4, standard drug (silymarin), and blood serum analysis kits were stocked. The in vivo tests were performed in 140 healthy Wister albino rats of either sex under well-controlled parameters divided into 14 groups, strictly maintaining Institutional Animal Ethics Committee (IEAC) protocols. For the histopathology study, 10% buffered neutral formalin was used for organ preservation. Later the specimens were studied under a fluorescence microscope. In silico molecular docking and absorption, distribution, metabolism, excretion, and toxicity (ADMET) studies were performed, and the results were analyzed statistically. Results and discussion Gynura procumbens partially negate the deleterious effect of carbon tetrachloride on normal weight gain in rats. The elevated level of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), creatinine, LDH, total cholesterol (TC), low-density lipoprotein (LDL), triglycerides (TG), malondialdehyde (MDA), deoxyribonucleic acid (DNA) fragmentation ranges, gamma-glutamyl transferase (γ-GT) in CCl4 treated groups were decreased by both standard drug silymarin and G. procumbens leaf extract. We have found significant & highly significant changes statistically for different doses, here p<0.05 & p<0.01, respectively. On the other hand, G. procumbens and silymarin displayed Statistically significant (p<0.05) and high significant(p<0.01) increased levels of HDL, CAT SOD (here p<0.05 & p<0.01 for different doses) when the treatment groups were compared with the disease control group. Because the therapeutic activity imparted by plants and drugs accelerates the movement of the disturbed pathophysiological state toward the healthy state. In the molecular docking analysis, G. procumbens phytoconstituents performed poorly against transforming growth factor-beta 1 (TGF-β1) compared to the control drug silymarin. In contrast, 26 phytoconstituents scored better than the control bezafibrate against peroxisome proliferator-activated receptor alpha (PPAR-α). The top scoring compounds for both macromolecules were observed to form stable complexes in the molecular dynamics simulations. Flavonoids and phenolic compounds performed better than other constituents in providing hepatoprotective activity. It can, thus, be inferred that the extract of G. procumbens showed good hepatoprotective properties in rats.

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“…The bark of Terminalia Arjuna has been extensively studied for its medicinal properties, and its extracts have been found to possess inotropic, anti-ischemic, antioxidant, blood pressure lowering, antiplatelet, hypolipidemic, antiatherogenic, and antihypertrophic activities [3][4][5]. These properties make it an attractive candidate for the development of novel therapeutic agents for cardiovascular diseases.…”

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confidence: 99%

Extraction, Characterization, and Biological Activities of Phytochemicals from Terminalia Arjuna (Arjuna) Plant

Atul Kumar Singh,

Arun Kumar

2024

JRPS

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This study focuses on the comprehensive characterization and biological activities of phytochemicals from the bark of Terminalia Arjuna (Arjuna). The bark, known for its medicinal properties, was extracted using methanol and analyzed for its phytochemical profile. The study employed various spectroscopic methods including Fourier transform infrared (FTIR), 1H NMR and 13C NMR spectroscopy. The antioxidant and anti-inflammatory activities were also evaluated while antimicrobial activity was assessed against both Gram-positive and Gram-negative bacteria. Additionally, the study included characterization by nuclear magnetic resonance (NMR) spectroscopy (1H and 13C) and infrared (IR) spectroscopy, as well as the determination of acid value, peroxide value, and the presence of aflatoxins. The results provide valuable insights into the potential therapeutic applications of Terminalia arjuna and its phytochemical constituents.

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An evaluation of pharmacological healing potentialities of Terminalia Arjuna against several ailments on experimental rat models with an in-silico approach

Cited by 5 publications

References 65 publications

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

An in vivo and in silico evaluation of the hepatoprotective potential of Gynura procumbens: A promising agent for combating hepatotoxicity

Extraction, Characterization, and Biological Activities of Phytochemicals from Terminalia Arjuna (Arjuna) Plant

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