An evaluation of antimicrobial potential of irreversible hydrocolloid impression material incorporated with chitosan

Abstract

Aim: To evaluate the antimicrobial potential of irreversible hydrocolloid impression material manipulated using chitosan impregnated solution at various time intervals. Setting and Design: Evaluative invivo study design. Materials and Methods: Maxillary impressions made for 20 dentulous volunteers using irreversible hydrocolloid impression material manipulated using distilled water as control and using 1% chitosan impregnated solution as test group using stock metal trays with one-week interval. Bacterial samples were collected using dry sterile cotton swab in the mid-palatal region at the time intervals of 0, 10, 20 minutes. Bacterial swabs were inoculated on nutrient agar media and incubated at 37° C for 24 hours. Bacterial colonies were counted with the aid of colony counter. Statistical Analysis Used: The resultant data was subjected to statistical analysis using repeated measures ANOVA and independent t test. Results: Adding water soluble chitosan to irreversible hydrocolloid impression material resulted in superior antimicrobial activity. With the passage of time there was a significant decrease in the microbial colony count upto 10min (p=0.016). However, the rate of decrease of microbial colony count was statistically insignificant between the samples collected at 10 and 20 min. Conclusion: Incorporation of water soluble chitosan to irreversible hydrocolloid impression material showed significant antimicrobial activity in 10 minutes.

Evaluation of antimicrobial property of nanochitosan coated orthodontic brackets against Streptococcus mutans and Lactobacillus acidophilus – an in vitro study

“…e result of the study coincided with previous studies that had demonstrated the antibacterial property of Chitosan and nano chitosan particles. [16][17][18][19][20][21][22][23][24] Chitosan exhibited potent antimicrobial properties against cariogenic bacteria when added to dentifrices, mouthwashes, composite resins, orthodontic bonding agents, and impression materials without affecting their mechanical and clinical properties. [16][17][18][19][20][21][22][23][24] Chitosan acts against bacteria by inhibiting the bacterial enzymes, causing chelation of metal ions and forming polyelectrolyte complexes on the bacterial cell wall.…”

“…[16][17][18][19][20][21][22][23][24] Chitosan exhibited potent antimicrobial properties against cariogenic bacteria when added to dentifrices, mouthwashes, composite resins, orthodontic bonding agents, and impression materials without affecting their mechanical and clinical properties. [16][17][18][19][20][21][22][23][24] Chitosan acts against bacteria by inhibiting the bacterial enzymes, causing chelation of metal ions and forming polyelectrolyte complexes on the bacterial cell wall. [17,[25][26][27][28][29][30] is specific action of chitosan against S. mutans and L. acidophilus may be due to the presence of more anionic peptidoglycan and Teichoic acid in the cell wall of gram-positive bacteria that interact with the cationic amino group of chitosan leading to cell death.…”

“…[16][17][18][19][20][21][22][23][24] Chitosan acts against bacteria by inhibiting the bacterial enzymes, causing chelation of metal ions and forming polyelectrolyte complexes on the bacterial cell wall. [17,[25][26][27][28][29][30] is specific action of chitosan against S. mutans and L. acidophilus may be due to the presence of more anionic peptidoglycan and Teichoic acid in the cell wall of gram-positive bacteria that interact with the cationic amino group of chitosan leading to cell death. [17,28] Intergroup comparisons revealed a significant difference between the mean colony counts of both the bacteria around the nanochitosan coated and uncoated brackets at all the time intervals tested [Table 4].…”

“…[17,[25][26][27][28][29][30] is specific action of chitosan against S. mutans and L. acidophilus may be due to the presence of more anionic peptidoglycan and Teichoic acid in the cell wall of gram-positive bacteria that interact with the cationic amino group of chitosan leading to cell death. [17,28] Intergroup comparisons revealed a significant difference between the mean colony counts of both the bacteria around the nanochitosan coated and uncoated brackets at all the time intervals tested [Table 4]. Studies have shown that coating orthodontic brackets with nanoparticles of metal and metal oxides with antibacterial properties reduced the number of cariogenic bacteria in an experimental setup.…”

Accuracy of Impression Material During COVID-19 after Sterilization- In vitro Study

“…As a result, it is critical to consider the sides that may be elaborated in the fabrication of prosthesis, one of which is elastomeric impression material [8]. The impression materials used to replicate the shape of the teeth and surrounding structures [9]. This delivers a transmission of highly infectious pathogens such as hepatitis B and C among others [10].…”