An evaluation of a mass transfer rate at the boundary of different release mechanisms in complex liquid dispersion

Markowska-Radomska, Agnieszka; Dłuska, E.

doi:10.1016/j.cep.2015.12.006

Cited by 11 publications

(6 citation statements)

References 43 publications

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“…The cryoprotectants were located within the internal/external/both water phases. A detailed description of this technique in the CTF‐apparatus can be found in papers . The biocontactor was sterilized with ethanol and then placed in the laminar class II biological safety cabinet (TopSafe‐Biohazard).…”

Section: Methodsmentioning

confidence: 99%

Cryoprotection and banking of living cells in a 3D multiple emulsion‐based carrier

Dłuska

Cui

Markowska-Radomska

et al. 2017

Biotechnology Journal

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The ability to preserve stem cells/cells with minimal damage for short and long periods of time is essential for advancements in biomedical therapies and biotechnology. New methods of cell banking are continuously needed to provide effective damage prevention to cells. This paper puts forward a solution to the problem of the low viability of cells during cryopreservation in a traditional suspension and storage by developing innovative multiple emulsion-based carriers for the encapsulation and cryopreservation of cells. During freezing-thawing processes, irreversible damage to cells occurs as a result of the formation of ice crystals, cell dehydration, and the toxicity of cryoprotectant. The proposed method was effective due to the "flexible" protective structure of multiple emulsions, which was proven by a high cell survival rate, above 90%. Results make new contributions in the fields of cell engineering and biotechnology and contribute to the development of methods for banking biological material.

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Section: Methodsmentioning

confidence: 99%

Cryoprotection and banking of living cells in a 3D multiple emulsion‐based carrier

Dłuska

Cui

Markowska-Radomska

et al. 2017

Biotechnology Journal

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“…The CTF apparatus provides high mass transfer parameters and uniform shear flow, contributing to the high encapsulation efficiency and formation of stable emulsions. 28,29,30 The structures of the obtained emulsions were analyzed using an Olympus BX60 optical microscope, with Olympus SC50 digital camera (Olympus, Japan), and image analysis software, Image Pro Plus 4.5 (Media Cybernetics, USA). For each of the double emulsions, at least 800 drops of the membrane phase and 1000 drops of the internal phase were measured.…”

Section: Preparation and Characterization Of Multiple Emulsions With Anti-cancer Drug (Drop Size Viscosity)mentioning

confidence: 99%

Mass transfer of anti-cancer drug delivery to brain tumours by a multiple emulsion-based implant

Dłuska¹,

Markowska-Radomska²,

Metera³

et al. 2021

Preprint

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The advanced use of a pH-responsive biomaterial-based injectable liquid implant for effective chemotherapeutic delivery in glioblastoma multiforme brain (GBM) tumour treatment is presented. As an implant, we proposed a water-in-oil-in-water multiple emulsion with encapsulated doxorubicin. The effectiveness of the proposed therapy was evaluated by comparing the cancer cell viability achieved in classical therapy (chemotherapeutic solution). The experimental study included doxorubicin release rates and consumption for two emulsions differing in drop sizes and structures in the presence of GBM-cells (LN229, U87 MG), and a cell viability. The results showed that the multiple emulsion implant was significantly more effective than classical therapy when considering the reduction in cancer cell viability: 85% for the emulsion-implant, and only 43% for the classical therapy. A diffusion-reaction model was adapted to predict doxorubicin release kinetics and elimination by glioblastoma cells. CFD simulations confirmed that the drug release kinetics depends on multiple emulsion structures and drop sizes.

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“…The method of multiple (double) emulsions formation via the one-step method in the Couette-Taylor flow contactor involves mixing internal water phase with membrane oil phase at the inlet cross section of contactor where simple emulsions are created and then introducing the external water phase to formulate multiple emulsions. The method has beeen described more precisely in our previous papers [10], [11].…”

Section: B Methods Of Formation Of the W 1 /O/w 2 Multiple Emulsionmentioning

confidence: 99%

Functionalized Multiple Emulsions as Platforms for Targeted Drug Delivery

Metera¹,

Dłuska²,

Markowska-Radomska³

et al. 2017

IJCEA

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Abstract-The paper presents the results of modification of outer interfaces of oil drops of multiple emulsions W 1 /O/W 2 for targeted drug delivery. The modification involves physical adsorption of antibodies: anti-CD15 (Hodgkin's disease marker). The effect of molecular interaction between molecules of antibody (protein) and oil phase compounds was measured using isothermal titration calorimetry (ITC). The ITC analysis also included investigation of interactions between components of emulsion and antibody buffer. Multiple emulsions were prepared by a one-step method, in a bioreactor with the Couette-Taylor flow. The inner phase of the emulsion was an aqueous solution of alginic acid, membrane phase was soybean oil, the outer phase was distilled water, appropriate surfactants were added to each phase. The influence of the structure of multiple emulsions (double emulsions with single internal drops and many internal drops) on adsorption of antibodies was investigated. The ITC analysis showed that antibodies interacted with emulsion compounds. The change in heat rates of molecular interactions suggested adhesion of protein onto oil drops interfaces.

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An evaluation of a mass transfer rate at the boundary of different release mechanisms in complex liquid dispersion

Cited by 11 publications

References 43 publications

Cryoprotection and banking of living cells in a 3D multiple emulsion‐based carrier

Cryoprotection and banking of living cells in a 3D multiple emulsion‐based carrier

Mass transfer of anti-cancer drug delivery to brain tumours by a multiple emulsion-based implant

Functionalized Multiple Emulsions as Platforms for Targeted Drug Delivery

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