Functionalized Multiple Emulsions as Platforms for Targeted Drug Delivery

Abstract

Abstract-The paper presents the results of modification of outer interfaces of oil drops of multiple emulsions W 1 /O/W 2 for targeted drug delivery. The modification involves physical adsorption of antibodies: anti-CD15 (Hodgkin's disease marker). The effect of molecular interaction between molecules of antibody (protein) and oil phase compounds was measured using isothermal titration calorimetry (ITC). The ITC analysis also included investigation of interactions between components of emulsion and antibody buffer. Multiple emulsions were prepared by a one-step method, in a bioreactor with the Couette-Taylor flow. The inner phase of the emulsion was an aqueous solution of alginic acid, membrane phase was soybean oil, the outer phase was distilled water, appropriate surfactants were added to each phase. The influence of the structure of multiple emulsions (double emulsions with single internal drops and many internal drops) on adsorption of antibodies was investigated. The ITC analysis showed that antibodies interacted with emulsion compounds. The change in heat rates of molecular interactions suggested adhesion of protein onto oil drops interfaces.

A novel drug loading and release from a thermoresponsive hydrogel formedin situemulsion polymerization

“…The obtained nanoparticles demonstrated an excellent loading and release of doxorubicin hydrochloride, which was quick in a weakly acidic environment but slightly slow in the basic media. In addition, multiple emulsions composed of an alginic acid solution as inner phase, water, and soybean oil as membrane phase were used as platforms to deliver doxorubicin and temozolomide in drug delivery application. Besides, studies have shown that the effectiveness of cancer chemotherapy treatment efficiency could be increased.…”