An Evaluation of 25B-, 25C-, 25D-, 25H-, 25I- and 25T2-NBOMe via LC–MS-MS: Method Validation and Analyte Stability

Abstract

As potent serotonin (5-HT2A) receptor agonists, the NBOMe class of drugs including 25B-, 25C-, 25D-, 25H-, 25I- and 25T2-NBOMe is frequently abused due to the intense hallucinations that they induce. From the limited literature available, the concentration of these NBOMe compounds reported in postmortem cases is exceedingly low. In most instances, published concentrations are <0.50 ng/mL. Therefore, the need for a sensitive, rapid and comprehensive analytical method for the quantification of these compounds was evident. In addition to the more publicized analog 25I-NBOMe, evaluation of 25B-, 25C-, 25D-, 25H and 25T2- in whole blood, plasma and urine was conducted. This publication presents the data obtained from the validation of a liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of these six NBOMe analogs. The method utilizes ultra-performance liquid chromatography technology for the separation followed by positive electrospray ionization of each analog. Limits of quantification for these analogs ranged from 0.01 to 0.02 ng/mL (10-20 pg/mL) with typical linear dynamic ranges of 0.01-20 ng/mL. Data for recovery, intraday control accuracy and precision, matrix effects, ion suppression/enhancement and analyte stability are included. Validation was completed in whole blood, plasma and urine. Short run times and high sensitivity afforded by this newly validated analytical method that allows for the detection of these six analogs in the most common toxicological matrices and can be applied to both ante- and postmortem specimens.

Next generation of novel psychoactive substances on the horizon – A complex problem to face

“…They include liquid chromatography-tandem mass spectrometry, liquid chromatography coupled to quadrupole-time-of flight mass spectrometry, gas chromatography/mass spectrometry, matrix-assisted/laser desorption/ionization time M a n u s c r i p t 5 of flight mass spectroscopy, direct analysis in real time mass spectrometry, nuclear magnetic resonance, immunoassays (e.g., Adamowicz and Tokarczyk, 2015;ElSohly et al, 2014;Johnson et al, 2014;Lanza et al, 2015;Poklis et al, 2014;Tang et al, 2014a).…”

Rapid Screening Method for New Psychoactive Substances of Forensic Interest: Electrochemistry and Analytical Determination of Phenethylamines Derivatives (NBOMe) via Cyclic and Differential Pulse Voltammetry

“…Only a few pharmacologic studies on humans have been conducted on these drugs. Reports of adverse effects after ingestion began to appear in the scientific literature in 2013, describing prominent neuropsychiatric effects and instability 6,[10][11][12][13][14][15][16] . In severe cases death can occur even after ingesting a single dose 14 .…”

Severe clinical toxicity caused by 25I-NBOMe confirmed analytically using LC-MS-MS method

“…A small active dose of 25I-NBOMe and its analogs leads to low concentrations of the compound in the organism, therefore analytical methods to detect and quantify this substance in bio-samples must be highly accurate, sensitive and specific. The most suitable method for this purpose is high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) (Johnson et al, 2014).…”