An Evaluation Method of Cleanness of Meat Processing Instruments based on Total ATP Determination.

Hisa, Kazuo; Madokoro, Hiroko; Honma, Shiho

doi:10.3136/nskkk.45.550

Cited by 3 publications

(1 citation statement)

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“…Some food samples show high ATP concentrations in comparison with their low microbial cell counts. This phenomenon can also be observed for swab samples of food processing instruments 13) . A possible explanation is that food samples can contain high concentrations of endogenous ATP, because most food materials are of animal or plant origin and may retain their original ATP content 14) .…”

Section: Introductionmentioning

confidence: 77%

New Estimation Methods of Bacterial Concentration by Measuring ATP Changes during Incubation.

Fujikawa¹,

Morozumi²

2002

Journal of the Food Hygienics Society of Japan

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New estimation methods of bacterial cell concentration in samples by measurement of the increase in bacterial adenosine-triphosphate (ATP) content during incubation using a conventional firefly luminometer were established. When an Escherichia coli cell suspension was incubated in nutrient broth, the increase in the ATP content of the suspension during the incubation period followed a sigmoidal curve. The increase ratio of the ATP content of the suspension at a given period of incubation (5 hours in this study) to the initial ATP content was greater at higher initial cell concentrations. With this relationship, the initial cell concentration of a test suspension could be predicted from the measured ratio; this was called the end point method. On the other hand, the lag period in the ATP increase curve was longer at lower initial cell concentrations. A highly linear relationship was observed between the lag period and the logarithm of the initial cell concentrations. Based on this relationship, a delay method was developed for prediction. The two relationships were also observed for bacterial suspensions of Klebsiella sp., Staphylococcus aureus, Bacillus subtilis, and Pseudomonas sp. These results suggested that the two methods have the potential to estimate the bacterial cell concentration of a sample suspension.

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Section: Introductionmentioning

confidence: 77%

New Estimation Methods of Bacterial Concentration by Measuring ATP Changes during Incubation.

Fujikawa¹,

Morozumi²

2002

Journal of the Food Hygienics Society of Japan

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Development of an ionization method using hydrogenated plasma for mass analysis of surface adhesive compounds

Aida

Iwai

Okamoto

et al. 2018

J. Anal. At. Spectrom.

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Development of a Dual Plasma Desorption/Ionization System for the Noncontact and Highly Sensitive Analysis of Surface Adhesive Compounds

et al. 2017

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We developed a dual plasma desorption/ionization system using two plasmas for the semi-invasive analysis of compounds on heat-sensitive substrates such as skin. The first plasma was used for the desorption of the surface compounds, whereas the second was used for the ionization of the desorbed compounds. Using the two plasmas, each process can be optimized individually. A successful analysis of phenyl salicylate and 2-isopropylpyridine was achieved using the developed system. Furthermore, we showed that it was possible to detect the mass signals derived from a sample even at a distance 50 times greater than the distance from the position at which the samples were detached. In addition, to increase the intensity of the mass signal, 0%–0.02% (v/v) of hydrogen gas was added to the base gas generated in the ionizing plasma. We found that by optimizing the gas flow rate through the addition of a small amount of hydrogen gas, it was possible to obtain the intensity of the mass signal that was 45–824 times greater than that obtained without the addition of hydrogen gas.

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An Evaluation Method of Cleanness of Meat Processing Instruments based on Total ATP Determination.

Cited by 3 publications

References 0 publications

New Estimation Methods of Bacterial Concentration by Measuring ATP Changes during Incubation.

New Estimation Methods of Bacterial Concentration by Measuring ATP Changes during Incubation.

Development of an ionization method using hydrogenated plasma for mass analysis of surface adhesive compounds

Development of a Dual Plasma Desorption/Ionization System for the Noncontact and Highly Sensitive Analysis of Surface Adhesive Compounds

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