An estimation and evaluation of total antioxidant capacity of saliva in children with severe early childhood caries

Abstract

BACKGROUND. The available evidence implicating the involvement of oxidative stress in the caries process suggests that local antioxidant status may be of importance in determining the susceptibility to the caries process. AIM. The aim of this study was to estimate the total antioxidant capacity (TAC) in unstimulated saliva of healthy children with and without severe early childhood caries (S-ECC) and to correlate the individual TAC level with dmft (d = decayed, m = missing, f = filled, t = teeth) score and age. MATERIAL AND METHODS. The TAC of saliva was investigated in 100 healthy children in the age range of 3-5 years divided in two groups, control and study group based on the absence or presence of caries, respectively. The antioxidant capacity of saliva was estimated by an adaptation of ABTS [2, 2'-Azino-di-(3-ethylbenzthiazoline sulphonate)] assay. RESULTS. The mean TAC level in the saliva of the children in study group was found to be significantly increased (P < 0.001), and a significantly linear regression was seen between the TAC and dmft score (P < 0.001) whereas it was insignificant between the TAC and age (P = 0.078). CONCLUSION. The results indicated that TAC of saliva increased significantly in children with S-ECC and increasing prevalence of dental caries predisposes to the increase in TAC of saliva.

Comparison of Total Antioxidant Capacity in Saliva of Children with Severe Early Childhood Caries and Caries-Free Children

“…Uberos et al [2008] showed that the TAC level in the saliva of patients with dental caries is 2.89 times more than in caries-free individuals. Recently, Kumar et al [2011] reported that the salivary TAC level in the study group with S-ECC in Indian children was significantly higher than the TAC level in caries-free children. However, they evaluated the salivary TAC level through another method (ABTS method), while the present study used a technique known as FRAP.…”

“…However, the studies were carried out in the field of some oxidative stress indicators in S-ECC [Kumar et al, 2011]. Therefore, more comprehensive studies in this regard need to be done in order to clarify the potential role of oxidative stress in the pathogenesis of this disease.…”

“…There was also an increment in TAC associated with increasing age of patients. Recently, Kumar et al [2011] evaluated the TAC in Indian children with S-ECC by the ABTS method. Their study showed that TAC in the saliva of children with early caries was significantly more than the amount seen in the caries-free children.…”

Salivary Biomarkers in Caries Affected and Caries Free Children

“…Moreover, a higher total antioxidant capacity (TAC) was observed with dental caries in deciduous but not in permanent teeth [17][18][19][20] and TAC increased with the age of the children [21,22].…”

Evaluation of Total Antioxidant Capacity (TAC) of Saliva in Caries Active and Caries free Tribal School Going Children of Wardha District.

“…Results of studies on TAC Tulunoglu et al [9] Total antioxidant values were higher in caries active groups Uberos et al [13] Total antioxidant capacity of the saliva of patients with caries in deciduous teeth was greater than among those without caries Prabhakar et al [14] Total antioxidant capacity of saliva increased significantly in caries active children Hegde et al [15] Total antioxidant capacity of saliva increased in children with caries Preethi et al [10] Total antioxidant capacity of saliva increased in caries active children Kumar et al [16] TAC of saliva increased in children with Severe early childhood caries and increasing prevalence of dental caries predisposes to the increase in TAC of saliva Dodwad et al [1] Total antioxidant capacity of saliva increased significantly in cariesactive children when compared to caries-free children Hegde et al1 [7] TAC level increases with increase in severity of caries Ahmadi-Motamayel et al [18] TAC was significantly higher in the saliva of caries active group relative to the caries free subjects Mahjoub et al1 [9] TAC levels and salivary total protein increased in children with severe early childhood caries compared with caries-free children Pandey et al [20] Total antioxidants in saliva increased with caries activity Muchandi et al [21] Salivary TAC increases in patients with early childhood caries Silva et al [22] Salivary Total Antioxidant capacity was higher in children with early childhood caries as compared to caries free children …”