An Escherichia coli enterobactin cluster gene with sequence homology to trpE and pabB

Elkins, M

doi:10.1016/0378-1097(88)90123-1

Cited by 12 publications

(14 citation statements)

References 9 publications

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“…In periplasmic transport systems the binding protein gene is generally part of an operon that also includes genes for membrane-bound components (2) The regulatory region between fepB and entC contains divergent promoters and two Fur boxes, as has been reported previously (13,29). We presume that the fepB promoter overlaps its Fur box, as is generally the situation for genes immediately downstream from Fur boxes, in which case fepB has a long leader RNA that contains ORF1.…”

Section: Discussionmentioning

confidence: 56%

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Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepB

Elkins

Earhart

1989

J Bacteriol

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Section: Discussionmentioning

confidence: 56%

“…The sequence upstream from fepB contains the promoter elements for both fepB and entC (13,29). This 417-nucleotide sequence contained two binding sites (Fur boxes) for the regulatory protein Fur; these 19-bp palindromic sequences occur upstream from many iron-regulated genes, and transcription of these genes is reduced upon binding of the FIG.…”

mentioning

confidence: 99%

Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepB

Elkins

Earhart

1989

J Bacteriol

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“…This compound is utilized for the biosynthesis of some quinones and phenolic acid derivatives such as 2,3-dihydroxybenzoic acid (2,3-DHB). ICSs cited above have been reported as key enzymes in the biosynthetic pathway of siderophores such as enterobactin (19), pyochelin and SA (56), amonabactin (6), and vibriobactin (O07898).…”

Section: Resultsmentioning

confidence: 99%

Analysis of the pmsCEAB Gene Cluster Involved in Biosynthesis of Salicylic Acid and the Siderophore Pseudomonine in the Biocontrol Strain Pseudomonas fluorescens WCS374

et al. 2001

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Mutants of Pseudomonas fluorescens WCS374 defective in biosynthesis of the fluorescent siderophore pseudobactin still display siderophore activity, indicating the production of a second siderophore. A recombinant cosmid clone (pMB374-07) of a WCS374 gene library harboring loci necessary for the biosynthesis of salicylic acid (SA) and this second siderophore pseudomonine was isolated. The salicylate biosynthesis region of WCS374 was localized in a 5-kb EcoRI fragment of pMB374-07. The SA and pseudomonine biosynthesis region was identified by transfer of cosmid pMB374-07 to a pseudobactin-deficient strain of P. putida. Sequence analysis of the 5-kb subclone revealed the presence of four open reading frames (ORFs). Products of two ORFs (pmsC and pmsB) showed homologies with chorismate-utilizing enzymes; a third ORF (pmsE) encoded a protein with strong similarity with enzymes involved in the biosynthesis of siderophores in other bacterial species. The region also contained a putative histidine decarboxylase gene (pmsA). A putative promoter region and two predicted iron boxes were localized upstream of pmsC. We determined by reverse transcriptase-mediated PCR that the pmsCEAB genes are cotranscribed and that expression is iron regulated. In vivo expression of SA genes was achieved in P. putida and Escherichia coli cells. In E. coli, deletions affecting the first ORF (pmsC) diminished SA production, whereas deletion of pmsB abolished it completely. The pmsB gene induced low levels of SA production in E. coli when expressed under control of the lacZ promoter. Several lines of evidence indicate that SA and pseudomonine biosynthesis are related. Moreover, we isolated a Tn5 mutant (374-05) that is simultaneously impaired in SA and pseudomonine production.

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“…The first step in this pathway is the conversion of chorismate to isochorismate, which is catalyzed by EntC, isochorismate synthase. The gene encoding this 43,000-molecular-weight monomeric protein has been cloned and sequenced (15,27,34), and EntC has homology with other enzymes which use chorismate as a substrate. Isochorismate is also a precursor in the synthesis of menaquinone, and a second isochorismate synthase gene, menF, has recently been identified in E. coli (12).…”

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confidence: 99%

Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis

et al. 1997

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Vibrio cholerae secretes the catechol siderophore vibriobactin in response to iron limitation. Vibriobactin is structurally similar to enterobactin, the siderophore produced by Escherichia coli, and both organisms produce 2,3-dihydroxybenzoic acid (DHBA) as an intermediate in siderophore biosynthesis. To isolate and characterize V. cholerae genes involved in vibriobactin biosynthesis, we constructed a genomic cosmid bank of V. cholerae DNA and isolated clones that complemented mutations in E. coli enterobactin biosynthesis genes. V. cholerae homologs of entA, entB, entC, entD, and entE were identified on overlapping cosmid clones. Our data indicate that the vibriobactin genes are clustered, like the E. coli enterobactin genes, but the organization of the genes within these clusters is different. In this paper, we present the organization and sequences of genes involved in the synthesis and activation of DHBA. In addition, a V. cholerae strain with a chromosomal mutation in vibA was constructed by marker exchange. This strain was unable to produce vibriobactin or DHBA, confirming that in V. cholerae VibA catalyzes an early step in vibriobactin biosynthesis.

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An Escherichia coli enterobactin cluster gene with sequence homology to trpE and pabB

Cited by 12 publications

References 9 publications

Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepB

Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepB

Analysis of the pmsCEAB Gene Cluster Involved in Biosynthesis of Salicylic Acid and the Siderophore Pseudomonine in the Biocontrol Strain Pseudomonas fluorescens WCS374

Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis

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