An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress

Yg, Shen; Wk, Zhang; Sj, He; Js, Zhang; Q, Liu; Sy, Chen

doi:10.1007/s00122-002-1131-x

Cited by 286 publications

(190 citation statements)

References 30 publications

(33 reference statements)

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“…Constitutive overexpression of some stress-responsive transcription factor genes, such as DREBs, controlled by the CaMV 35S promoter frequently caused unwanted phenotypes, such as reduced plant growth, that finally caused significant reduction of potential yield (37). However, transgenic plants (T 0 ) or families (T 1 and T 2 ) of SNAC1 showed no obvious difference from the WT plants in all of the traits investigated.…”

Section: Discussionmentioning

confidence: 99%

Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice

Hu¹,

Dai²,

Yao

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,352

1,020

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Drought and salinity are major abiotic stresses to crop production. Here, we show that overexpression of stress responsive gene SNAC1 (STRESS-RESPONSIVE NAC 1) significantly enhances drought resistance in transgenic rice (22-34% higher seed setting than control) in the field under severe drought stress conditions at the reproductive stage while showing no phenotypic changes or yield penalty. The transgenic rice also shows significantly improved drought resistance and salt tolerance at the vegetative stage. Compared with WT, the transgenic rice are more sensitive to abscisic acid and lose water more slowly by closing more stomatal pores, yet display no significant difference in the rate of photosynthesis. SNAC1 is induced predominantly in guard cells by drought and encodes a NAM, ATAF, and CUC (NAC) transcription factor with transactivation activity. DNA chip analysis revealed that a large number of stress-related genes were up-regulated in the SNAC1-overexpressing rice plants. Our data suggest that SNAC1 holds promising utility in improving drought and salinity tolerance in rice.Oryza sativa ͉ abscisic acid ͉ stomata ͉ dehydration P oor water management, increased competition for limited water resources, and the uncertain threats associated with global warming all highlight the looming water crisis that threatens agricultural productivity worldwide. In China alone, the estimated annual loss of national economy from water shortage alone reaches Ͼ$25 billion (1). In addition to altered water management practices, the ability to enhance the tolerance of crops to drought and salinity stress, particularly at the most sensitive reproductive stage of growth, can have a potentially huge impact on productivity in the years to come.Plants can develop numerous physiological and biochemical strategies to cope with adverse conditions (2, 3). The major events of plant response to dehydration stresses are perception and transduction of the stress signals through signaling components, resulting in activation of a large number of stress-related genes and synthesis of diverse functional proteins that finally lead to various physiological and metabolic responses (4-6). Well characterized proteins involved in the protection of plant cells from dehydration stress damage include molecule chaperons, osmotic adjustment proteins (7), ion channels (8), transporters (9), and antioxidation or detoxification proteins (10). The expression of these functional proteins is largely regulated by specific transcription factors (4, 11).More than 30 families of transcription factors have been predicted for Arabidopsis (12). Members of DREB or CBF, MYB, bZIP, and zinc-finger families have been well characterized with roles in the regulation of plant defense and stress responses (4-6, 13, 14). Most of these transcription factors regulate their target gene expression through binding to the cognate cis-elements in the promoters of the stress-related genes. Two well characterized dehydration stress-related cis-elements bound by transcription factors are...

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Section: Discussionmentioning

confidence: 99%

Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice

Hu¹,

Dai²,

Yao

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,352

1,020

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“…In Arabidopsis and rice, the subtype 1 genes DREB2A and OsDREB2B have the highest stress inducibility among the DREB2 genes and have been shown to play significant roles in DREmediated stress responses in both plant species (Sakuma et al, 2002(Sakuma et al, , 2006a(Sakuma et al, , 2006bMatsukura et al, 2010). In addition, many stress-inducible DREB2-type transcription factors that have been identified to date belong to subtype 1 (Shen et al, 2003;Xue and Loveridge, 2004;Egawa et al, 2006;Agarwal et al, 2007;Qin et al, 2007;Matsukura et al, 2010). Therefore, we focused on the eight subtype 1 genes: GmDREB2A;1, GmDREB2A;2, GmDREB2B;1, GmDREB2B;2, GmDREB2C;1, GmDREB2C;2, GmDREB2D;1, and GmDREB2D;2.…”

Section: Identification and Classification Of Dreb2-type Transcriptiomentioning

confidence: 99%

“…The inducibility in response to stress conditions is a common feature among DREB2-type transcription factors of various plants, and the promoter structure including these cis-elements is conserved among many eudicot species Mizoi et al, 2012). In Poaceae (grass family), posttranscriptional control by alternative splicing works as a key regulatory mechanism of DREB2-type transcription factors, although this alternative splicing mechanism is not found in other plant families (Shen et al, 2003;Qin et al, 2007;Matsukura et al, 2010). In Arabidopsis, the accumulation of DREB2A mRNA is not sufficient for the induction of downstream genes, and posttranslational regulation is important in activating DREB2A (Liu et al, 1998;Sakuma et al, 2006a).…”

mentioning

confidence: 99%

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

et al. 2012

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Soybean (Glycine max) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, and production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2) group includes transcription factors that contribute to drought and heat stress tolerance by activating transcription through the cis-element dehydration-responsive element (DRE) in response to these stress stimuli. Two modes of regulation, transcriptional and posttranslational, are important for the activation of gene expression by DREB2A in Arabidopsis (Arabidopsis thaliana). However, the regulatory system of DREB2 in soybean is not clear. We identified a new soybean DREB2 gene, GmDREB2A;2, that was highly induced not only by dehydration and heat but also by low temperature. GmDREB2A;2 exhibited a high transactivation activity via DRE and has a serine/threonine-rich region, which corresponds to a negative regulatory domain of DREB2A that is involved in its posttranslational regulation, including destabilization. Despite the partial similarity between these sequences, the activity and stability of the GmDREB2A;2 protein were enhanced by removal of the serine/threonine-rich region in both Arabidopsis and soybean protoplasts, suggestive of a conserved regulatory mechanism that involves the recognition of serine/ threonine-rich sequences with a specific pattern. The heterologous expression of GmDREB2A;2 in Arabidopsis induced DREregulated stress-inducible genes and improved stress tolerance. However, there were variations in the growth phenotypes of the transgenic Arabidopsis, the induced genes, and their induction ratios between GmDREB2A;2 and DREB2A. Therefore, the basic function and regulatory machinery of DREB2 have been maintained between Arabidopsis and soybean, although differentiation has also occurred.

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“…Earlier studies demonstrated that the expression of the CBF genes in Arabidopsis resulted in an increased tolerance to freezing, salt and drought stresses (Liu et al 1998;Jaglo-Ottesen et al 1998;Kasuga et al 1999;Gilmour et al 2000). The cDNAs encoding CBF transcription factors have been identified and cloned from various plant species including Arabidopsis, rice, wheat, canola, soybean (Liu et al 1998;Dubouzet et al 2003;Shen et al 2003;Li et al 2005). In the present investigation, we have cloned and characterized the first member of CBF gene Fig.…”

Section: Discussionmentioning

confidence: 84%

“…DREB2-like genes, belong to the A-2 subgroup, are mainly involved in regulation of genes responsive to osmotic stress (Sakuma et al 2002;Nakashima and Yamaguchi-Shinozaki 2006). Arabidopsis homologs of DREB1/CBF genes have been isolated and characterised in evolutionarily diverse plants with different levels of adaptation to cold stress such as, common wheat (Triticum aestivum L.), rice (Oryza sativa L.), rye (Secale cereale L.), maize (Zea mays L.), canola (Brassica napus), etc., and overexpression of DREB1/CBF genes in transgenic plants increases tolerance to drought, high salt, and freezing stresses (Dubouzet et al 2003;Shen et al 2003;Qin et al 2004;Zhang et al 2004a;Hong and Kim 2005;Benedict et al 2006;Zhao et al 2006;Chinnusamy et al 2010). So, CBF genes may be useful for improving the stress tolerance of crops (Yamaguchi- .…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.

Akhtar

Jaiswal

et al. 2013

Physiol Mol Biol Plants

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The dehydration-responsive element-binding (DREB) protein/C-Repeat Binding Factors (CBFs) belongs to APETALA2 (AP2) family transcription factors that binds to DRE/CRT cis-element in cold-responsive (COR) genes and induce COR genes. CBFs have been isolated and characterized from evolutionarily diverse plant species. CBF pathway is conserved by CBF regulon and the size or the number and kind of target genes vary among freezing sensitive and tolerant plants. Hence, cloning of CBFs from highly freezing tolerant plants such as Lepidium latifolium L. will be useful in understanding the freezing tolerance of this species. In this study, LlCBF, a CBF1 family gene from L. Latifolium L., was cloned using RT-PCR and RACE-PCR. The full length mRNA of LlCBF is 948 bp with an open reading frame of 642 bp, encoding a protein of 213 amino acids with a molecular weight of 23.92 kDa and a theoretical isoelectric point of 4.80. Amino acid sequence analysis showed that LlCBF has an AP2 DNA binding domain, a potential CBF type nuclear localization signal (NLS) and C-terminal acidic domain. Semi-quantitative RT-PCR analysis of LlCBF revealed that this gene is up-regulated by high salt, dehydration and low temperature stresses. The investigation is therefore successful in cloning of a gene having strong homology with CBF transcription factors and responsive to low temperature, high salt and dehydration conditions.

show abstract

An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress

Cited by 286 publications

References 30 publications

Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice

Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.

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