Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L.

Abstract

The dehydration-responsive element-binding (DREB) protein/C-Repeat Binding Factors (CBFs) belongs to APETALA2 (AP2) family transcription factors that binds to DRE/CRT cis-element in cold-responsive (COR) genes and induce COR genes. CBFs have been isolated and characterized from evolutionarily diverse plant species. CBF pathway is conserved by CBF regulon and the size or the number and kind of target genes vary among freezing sensitive and tolerant plants. Hence, cloning of CBFs from highly freezing tolerant plants such as Lepidium latifolium L. will be useful in understanding the freezing tolerance of this species. In this study, LlCBF, a CBF1 family gene from L. Latifolium L., was cloned using RT-PCR and RACE-PCR. The full length mRNA of LlCBF is 948 bp with an open reading frame of 642 bp, encoding a protein of 213 amino acids with a molecular weight of 23.92 kDa and a theoretical isoelectric point of 4.80. Amino acid sequence analysis showed that LlCBF has an AP2 DNA binding domain, a potential CBF type nuclear localization signal (NLS) and C-terminal acidic domain. Semi-quantitative RT-PCR analysis of LlCBF revealed that this gene is up-regulated by high salt, dehydration and low temperature stresses. The investigation is therefore successful in cloning of a gene having strong homology with CBF transcription factors and responsive to low temperature, high salt and dehydration conditions.

The Trials and Tribulations of the Plant Male Gametophyte — Understanding Reproductive Stage Stress Tolerance

“…Sporopollenin precursors are deposited by ABC transporters that are expressed in the tapetum at the early vacuolated microspore stage [134,141]. Sporopollenin biosynthetic enzymes form a complex ('metabolon') in the endoplasmatic reticulum of the tapetum [142]. Recent ultrastructural studies reveal the involvement of specialised tapetum organelles, elaioplasts or tapetosomes, in exine wall deposition [140,143].…”

Genome-wide identification and expression analysis of the CBF transcription factor family in Lolium perenne under abiotic stress

“…For example, expression of an LlCBF genes from Lepidium latifolium was found to be enhanced in response to high salinity, dehydration, and low-temperature treatments. 9 Similarly, among the six CBF subfamily genes from P. persi ca ( PpCBF1-6 ), the expression of PpCBF1 , −5, and −6 was observed to be induced by exposure to low temperature, whereas that of the other three PpCBF genes remained relatively constant. 10 …”