An Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Avian Adenovirus and Avian Adenovirus-Associated Virus in Chickens

Dawson, George J.; Orsi, L. N.; Yates, Vance J.; Chang, P. W.; Pronovost, A. D.

doi:10.2307/1589706

Cited by 39 publications

(8 citation statements)

References 13 publications

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“…(Meulemans and Halen, 1982;York et al, 1983). Although non-specific binding by avian antibodies have been described with several ELISA tests using avian sera (Slaght et al, 1979;Dawson et al, 1980;York et al, 1983), it was not a problem in the test described here probably because each serum sample was tested in parallel on negative control antigen as well as on positive antigen, with the results expressed as positive-negative absorbance difference.…”

Section: Comparison Of Tests With Serially Diluted Seramentioning

confidence: 96%

Comparison of serological tests for detection of antibodies to infectious laryngotracheitis virus

et al. 1985

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SUMMARYFour serological tests i.e. ELISA, serum neutralisation (SN), fluorescent antibody (FA), and agar gel immunodiffusion (AGID) were compared for sensitivity using several criteria, for detection and titration of infectious laryngotracheitis (ILT) virus antibodies in chicken sera. In the ELISA test, sera were tested in parallel on virus positive and negative control antigens with results expressed as positive-negative difference. Non-specific binding was not a problem in this test. SN tests were performed in microtitre plates using chick embryo liver cells, while sera for FA tests were titrated on multispot slides on which were fixed ILT virus-infected chick embryo liver cell cultures. The AGID test was the standard test still widely used for serological diagnosis of ILT.The four tests were compared using (1) sera from experimentally inoculated birds bled regularly at intervals from 4 to 35 days post-inoculation, (2) convalescent sera from a natural outbreak of ILT, and (3) serial dilutions of an ILT positive serum. In all experiments the ELISA test was of slightly greater sensitivity than SN and was comparable to the FA test. In the experimentally infected birds ELISA and FA test detected sero-conversion in more birds at 7 days than SN. In tests with the serially diluted hyperimmune ILT serum, ELISA, FA and SN tests were comparable. SN however was the most useful test for quantification of ILT antibodies. ILT-SN titres in birds were never high, the highest titre recorded in experimental birds and in convalescent sera was 1/48. AGID was found to be less sensitive than ELISA, FA or SN test but was considered useful for detection of antibodies on a flock basis, since, with the convalescent sera AGID detected a significant proportion of positives.

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Section: Comparison Of Tests With Serially Diluted Seramentioning

confidence: 96%

Comparison of serological tests for detection of antibodies to infectious laryngotracheitis virus

et al. 1985

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“…In poultry virology, this test has been shown to be of value to measure antibodies against infectious bronchitis virus (Mockett and Darbyshire, 1980;Soula and Moreau, 1981), reovirus (Slaght et al, 1978), infectious bursal disease virus (Marquardt et al, 1980;Howie and Thorsen, 1981), rotavirus (Bartz etal, 1980) and adenovirus (Dawson et al, 1980). The present paper describes the application of the ELISA to the detection of antibodies against infectious laryngotracheitis (ILT) virus in chicken serum.…”

Section: Introductionmentioning

confidence: 99%

Enzyme‐linked immunosorbent assay (ELISA) for detecting infectious laryngotracheitis viral antibodies in chicken serum

Meulemans¹,

Halen²

1982

Avian Pathology

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“…An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies against fowl adenovirus in 1980 (Dawson et al, 1980), TCoV in 1989 , and enterovirus in 1993 (Hayhow & Saif, 1993). And an association of this technique with an immunohistochemistry test demonstrated HEV distribution on organism and the pathogenesis of the disease in inoculated turkeys (Silim & Thorsen, 1981).…”

Section: Enteric Viruses In Turkey Flocks: a Historic Reviewmentioning

confidence: 99%

Enteric viruses in turkey flocks: a historic review

Alavarez

Ferreira

2014

Rev. Bras. Cienc. Avic.

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In this review, diagnostic techniques and viral agents involved in enteric diseases affecting turkeys are described. Data from field observations and laboratory researches have been reported in turkey flocks for over 70 years, and several viruses have been identified. After a period of 30 years of inoculation experiments and neutralization studies, adequate visualization of the viruses was achieved using electronic microscopy. During the following years, several studies were then conducted to isolate and classify those viruses using cell-culture, embryo-propagation, serological tests, genome electropherotyping by polyacrylamide gel electrophoresis of double-stranded RNA viruses, and recently, nucleic acid studies. Thus, since the 1990s, the nucleicacid technology has focused on genomic surveys and on the detection of specific segments of the genome of each virus using the polymerasechain reaction, resulting in several prevalence studies and phylogenetic analyses of different isolates and proper classification of the viruses.

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An Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Avian Adenovirus and Avian Adenovirus-Associated Virus in Chickens

Cited by 39 publications

References 13 publications

Comparison of serological tests for detection of antibodies to infectious laryngotracheitis virus

Comparison of serological tests for detection of antibodies to infectious laryngotracheitis virus

Enzyme‐linked immunosorbent assay (ELISA) for detecting infectious laryngotracheitis viral antibodies in chicken serum

Enteric viruses in turkey flocks: a historic review

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