An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for <i>Bordetella bronchiseptica</i> antibodies

Boot, R.; Bakker, Richard G.; Thuis, H. C. W.; Veenema, J. L.

doi:10.1258/002367793780745624

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…Furthermore, Jenkins (1977) who tested cross reactivity of ELISA, showed that 2.7% and 13% serum samples from growing pigs and mature hogs, respecively, reacted with Pm antigen. Boot et al (1993) suggested that ELISA be used in cases of monitoring infection caused by Bbr in rabbits and guinea pigs.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Bordetella bronchiseptica strains isolated from pigs in Poland

Stępniewska¹,

Urbaniak²,

Markowska-Daniel³

2014

Polish Journal of Veterinary Sciences

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A total of 209 Bordetella bronchiseptica (Bbr) strains isolated from pigs were examined. Phenotypic study included: biochemical characterization (motility, catalase, oxidase, urease activity, nitrate reduction and growth on MacConkey agar) and antimicrobial susceptibility (disc diffusion method). Genotypic studies based on detection of three genes encoded virulence factors, such as: flagella (fla), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR.Most of the Bbr strains tested had a homogeneous biochemical profile. 97.6% of them provided suitable results in biochemical tests. All Bbr isolates tested showed high resistance to penicillin (100%), linco-spectin (100%) and ceftiofur (97.9%). Over 57% and 43% of Bbr strains were resistant to ampicillin and amoxicillin, respectively. All Bbr isolates showed high sensitivity to most chemotherapeutics used such as enrofloxacin (97.9%), tetracycline (97.9%), oxytetracycline (97.9%), amoxicillin with clavulonic acid (95.8%), florfenicol (90.4%), and gentamicine (77.6%). Over of 94% of Bbr strains were moderately susceptible to norfloxacine.Molecular analysis confirmed that almost all evaluated Bbr strains (94.7%) possessed the fla gene. A lower percentage of isolates had the dnt gene (72.7%) and the lowest percentage of strains (51.7%), had the bfrZ gene.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Bordetella bronchiseptica strains isolated from pigs in Poland

Stępniewska¹,

Urbaniak²,

Markowska-Daniel³

2014

Polish Journal of Veterinary Sciences

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“…The method of Boot et al [16] was followed to prepare phase I antigen from B. bronchiseptica (MTCC-6838). The stock antigen was diluted 1 : 20 in 0.5% formal saline to OD 269 0.5.…”

Section: Methodsmentioning

confidence: 99%

Occurrence ofBordetellaInfection in Pigs in Northern India

Kumar

Singh

Bhardwaj

et al. 2014

International Journal of Microbiology

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Bordetella bronchiseptica infection causing atrophic rhinitis in pigs is reported from almost all countries. In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). Bordetella bronchiseptica could be isolated from six (1.92%) nasal swabs. Although isolates varied significantly in their antimicrobial sensitivity, they had similar plasmid profile. The genus specific and species specific amplicons were detected from 8.2% and 4.4% nasal swabs using mPCR with alc gene (genus specific) and fla gene and fim2 gene (species specific) primers, respectively. Observations revealed that there may be other bordetellae infecting pigs because about 50% of the samples positive using mPCR for genus specific amplicons failed to confirm presence of B. bronchiseptica. Of the pig sera tested with MAT and ELISA for Bordetella antibodies, 67.6% and 86.3% samples, respectively, were positive. For antigen detection mPCR was more sensitive than conventional bacterioscopy while for detection of antibodies neither of the two tests (MAT and ELISA) had specificity in relation to antigen detection. Study indicated high prevalence of infection in swine herds in Northern India.

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“…B. bronchiseptica была обнаружена в Японии, Китае, Западной Европе и других странах. В РФ в связи с недостаточностью изучения и условно-патогенной категорией, заболевания кроликов, вызванные данным патогеном, зафиксированы не были [13][14][15][16]…”

Section: результаты и обсуждениеunclassified

Risk-based approach to the health monitoring of rabbits

Bondareva,

Makarova,

Akimov

et al. 2023

Lab Anim Sci Rus

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Rabbits are recognized as classic laboratory animals and are widely used in many different researches. Laboratory rabbits are used both as independent test systems and as additional (duplicate) test systems with other small rodents. For the past few years, laboratory rabbits have been ranked 3rd among mammalian laboratory animals in terms of the number of uses for research and testing, for scientific and educational purposes after rats and mice. In order for the test system to be appropriate, it is necessary to create condi-tions for keeping, feeding and drinking for animals that meet their needs. It is also necessary to control their microbiological status and to do good veterinary care. The first question that arises with planning the health monitoring program of laboratory rabbits, is the question of the list of pathogens for these animals. Because of Russia does not have a recommended list of pathogens for laboratory animals, local preclinical centers are forced to use international recommendations (in particular, FELASA), which consist of data from different regions of Europe and do not include data of infectious diseases of the Russian Federation. At the same time, the authors of FELASA note the need to take into account the epidemiological and epizootological characteristics of each of the regions, depending on the geographical location. To substantiate the list of controlled pathogens of laboratory rabbits used in Russia, we applied a risk-based approach. For analysis, we used the FMEA/FMECA method (Failure Mode Effect Analysis/Failure Mode Effect Criticality Analysis) — Analysis of the types and consequences of potential inconsistencies. This article considers a list of pathogenic microorganisms for control in laboratory rabbits used in Russia. The results can be used for the health monitoring of laboratory rabbits in local research centers.

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An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies

Cited by 11 publications

References 11 publications

Phenotypic and genotypic characterization of Bordetella bronchiseptica strains isolated from pigs in Poland

Phenotypic and genotypic characterization of Bordetella bronchiseptica strains isolated from pigs in Poland

Occurrence ofBordetellaInfection in Pigs in Northern India

Risk-based approach to the health monitoring of rabbits

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