An enzyme-linked immunosorbent assay (ELISA) that discriminates between the venoms of Brazilian Bothrops species and Crotalus durissus

Chávez-Olórtegui, Carlos; Penaforte, C.L.; Silva, Rosemary R.; Ferreira, Ana Paula; Rezende, Nilton Alves de; Amaral, Carlos Faria Santos; Diniz, Carlos R.

doi:10.1016/s0041-0101(96)00119-5

Cited by 23 publications

(13 citation statements)

References 11 publications

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“…However, monoclonal antibody has not yet been widely used in venom identification because: (1) some monoclonal antibodies still present non-specific reactivity (Arumäe et al, 1987;Alape-Girón et al, 1994;Tanjoni, et al, 2003;Stábeli, et al, 2005); (2) monoclonal antibodies may not always be absorbed well to plastic surfaces and labeled by enzyme successfully (Ho et al, 1986); and (3) the single venom component may undergo degradation due to the metabolic process in a victim's body, and the degraded toxin cannot be recognized by the monoclonal antibody (Selvanayagam and Gopalakrishnakone, 1999). An alternative preparation widely used in venom identification is the species-specific polyclonal antibody (SSAb), which is obtained by passing the commercial antiserum through a heterologous venom-conjugated medium system (Heneine, et al, 1990;Ge et al, 1991;Chávez-Olórtegui et al, 1997;Le et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…To improve the specificity, the cross-reacting antibodies should be eliminated thoroughly by passing through the medium coupled with heterologous venoms individually (Heneine and Catty, 1993;Chávez-Olórtegui et al, 1997;Le et al, 2003). The cross-reacting venom antibodies are eliminated in several ELISA test kits developed since the 1970s, including one commercially available kit for identification of the envenomations caused by five major venomous snakes in Australia (Currie, 2004), and the kits developed by De (1996) and Le et al (2003) to detect bites from four venomous snakes in India and Vietnam.…”

Section: Introductionmentioning

confidence: 99%

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Immunoreactivity between venoms and commercial antiserums in four Chinese snakes and venom identification by species-specific antibody

Gao

Wang

et al. 2013

Journal of Immunological Methods

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunoreactivity between venoms and commercial antiserums in four Chinese snakes and venom identification by species-specific antibody

Gao

Wang

et al. 2013

Journal of Immunological Methods

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“…Typification of venoms usually employs gel-based chromatographic fingerprint profiles 19 or ELISA methods. 20,21 Chromatography is, however, time consuming whereas ELISA selectivity is limited and fine differentiation among species is normally not feasible. More recently, however, gene cloning and sequencing, 5 -7 and mass spectrometry (MS) 12,22,23 have being increasingly used to characterize peptides in snake venoms.…”

Section: Introductionmentioning

confidence: 99%

Peptide fingerprinting of snake venoms by direct infusion nano‐electrospray ionization mass spectrometry: potential use in venom identification and taxonomy

et al. 2008

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Fingerprinting by mass spectrometry has been increasingly used to study venom variations and for taxonomic analyses based on venom components. Most of these studies have concentrated on components heavier than 3 kDa, but Bothrops snake venoms contain many biologically active peptides, principally C-type natriuretic peptides and bradykinin-potentiating peptides (BPPs). In this work, we have examined the peptide profile of Bothrops venoms (B. alternatus, B. erythromelas, B. insularis, B. jararaca, B. jararacussu, B. leucurus and B. moojeni) using direct infusion nano-electrospray ionization mass spectrometry (nano-ESI-MS) subjecting the data further to principal components analysis (PCA) to assess whether the peptide distributions are reliable in distinguishing the venoms. ESI-MS of a low molar mass fraction obtained by ultrafiltration of each venom (5 kDa nominal cutoff filters) revealed that the venoms have a variety of peptides in common but that each venom also contains taxonomic marker peptides not shared with other venoms. One BPP peptide, QGGWPRPGPEIPP, was found to be common to the seven Bothrops species examined. This peptide may represent a specific marker for this genus since it was not found in the venom of the South American rattlesnake, Crotalus durissus terrificus. PCA on the ESI-MS data reveals a close relationship between B. jararaca, B. jararacussu and B. moojeni venoms, with B. leucurus and B. erythromelas being more distant from these three; B. alternatus and B. insularis were also located distant from these five species, as was C. d. terrificus. These results agree partially with established phylogenetic relationships among these species and suggest that ESI-MS peptide fingerprinting of snake venoms coupled with PCA is a useful tool for identifying venoms and for taxonomic analyses.

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“…El pseudoofidismo, puede llegar a ser un problema serio, en cuanto lleva a un sobre diagnóstico y por tanto a un tratamiento inadecuado con aumento de la morbilidad y costos hospitalarios, por lo que la necesidad de métodos diagnósticos más precisos y rápidos, que permitan al profesional de salud un manejo oportuno y adecuado se hace imperativa. En los últimos años se han desarrollado métodos inmunológicos que permiten identificar el tipo de veneno inoculado usando técnicas de ELISA (examen de inmuno-absorción ligado a enzimas) y RIA (radio inmuno-análisis) que poseen una gran sensibilidad y especificidad (17,18).…”

Section: Discussionunclassified

“…El tiempo que demora la atención luego de ocurrida la mordedura es muy importante, ya que existe una relación directamente proporcional entre este y el numero de complicaciones y secuelas (10,18,30).…”

Section: Discussionunclassified

Ofidismo en la provincia de Chanchamayo, Junín: Revisión de 170 casos consecutivos en el Hospital de Apoyo de La Merced.

Forero¹,

Várgas²,

Samame³

et al. 2013

Rev Med Hered

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Objetivo: Describir las características clínico-epidemiológicas del ofidismo en la provincia de Chanchamayo, Junín, Perú. Materiales y métodos: Se revisaron todas las historias clínicas de pacientes con diagnóstico de ofidismo en el “Hospital de Apoyo de La Merced” (HALM), Junín, Perú entre enero de 1998 y diciembre del 2000. Se recogieron datos de demográficos y clínico-epidemiológicos. Resultados: Las historias clínicas revisadas fueron 195, de estas, 170 fueron incluidas en el análisis. La media edad fue 26.2 años (rango: 1-76 años). La mayoría (62.4%) era de sexo masculino. El 43.5% provenía del distrito de La Merced. Los casos de ofidismo ocurrieron con mayor frecuencia (67%) durante los meses de lluvia (de diciembre a mayo). La localización más frecuente de la mordedura fue en los miembros inferiores (67.7%). El tiempo entre el accidente y la atención médica fue en promedio de 5 hrs. 43 min. (± 5 hrs. 56 min.). El animal agresor se identificó en 37.6% de las veces, siendo el más frecuente el Bothrops atrox (36.5%). Los síntomas más comunes que acompañaron el cuadro clínico fueron dolor, edema, eritema (80%). La mayoría (78.3%) recibió suero antiofídico, de estos, 18.8% presentaron reacciones anafilactoides o urticariformes luego de la administración. La complicación más frecuente fue celulitis, presentándose mas comúnmente en pacientes que recibieron corticoides por más de 5 días (p=0.024). Ningún paciente falleció. Conclusiones: La mayoría de accidentes ofídicos en esta región ocurren en el ámbito rural. La utilización de corticoides por más de 5 días se asoció con una mayor frecuencia de celulitis. Las reacciones de hipersensibilidad hacia el suero antiofídico no son raras, siendo recomendable realizar la prueba intradérmica antes de su administración.

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An enzyme-linked immunosorbent assay (ELISA) that discriminates between the venoms of Brazilian Bothrops species and Crotalus durissus

Cited by 23 publications

References 11 publications

Immunoreactivity between venoms and commercial antiserums in four Chinese snakes and venom identification by species-specific antibody

Immunoreactivity between venoms and commercial antiserums in four Chinese snakes and venom identification by species-specific antibody

Peptide fingerprinting of snake venoms by direct infusion nano‐electrospray ionization mass spectrometry: potential use in venom identification and taxonomy

Ofidismo en la provincia de Chanchamayo, Junín: Revisión de 170 casos consecutivos en el Hospital de Apoyo de La Merced.

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