An enzyme linked immunosorbent assay for measurements of lactoferrin in duodenal aspirates and other biological fluids

Abstract

A sensitive and specific enzyme linked immunoassay for lactoferrin is described. The assay is designed to estimate lactoferrin concentrations in samples of duodenal aspirate, but is suitable for other biological fluids as well. The detection limit was 4.7 pmol/l. Within and between assay reproducibility was 41 and 178 pmol/l (1 SD), respectively, at a concentration of 1974 pmol/l. In the fasting state the concentration of lactoferrin in duodenal aspirates of three normal subjects ranged from 0-390 pmol/l and increased to 2860-14,820 pmol/l after pancreatic stimulation.

Pathophysiology of rhinitis. Lactoferrin and lysozyme in nasal secretions.

“…It was first described in bovine milk in 1966 (14). In the human Lf has been found in tears, nasal secretions, bronchial mucus, urine, seminal fluid, uterine cervical mucus, hepatic bile, duodenal contents, pancreatic juices, and in the specific granules of neutrophils (15). In all of the secretions the levels of Lf far exceed the levels of transferrin, suggesting that while transferrin is the major iron-binding protein in the serum, Lf plays the major role in external secretions (14).…”

“…In tears, Lf levels range between 1 and 3 mg/ml (I12-14, 19-2 1), accounting for -15-30% of the TP (13). In human milk Lf is found at -1 mg/ml (15). In bronchial secretions Lf accounts for as much as 11.5% of the TP (22).…”

Functional polymorphisms in the LTF gene and risk of coronary artery stenosis

“…All samples were sequenced using the forward primer, and samples with heterozygosities were also resequenced using the reverse primer. Plasma lactoferrin was measured by enzyme-linked immunoassay as previously described [9].…”

Multiple inflammatory markers in patients with significant coronary artery disease

“…Neutrophil-activating peptide-2 was measured in citrate-theophylline-adenosine-dipyridamole-containing plasma (Diatube-H, Diagnostica Stago, Asnieres-sûr-Seine, France). The granulocyte activation products myeloperoxidase and lactoferrin were quantified as previously described [5,6]. To our knowledge, circulating concentrations of the terminal complement complex and lactoferrin have not previously been investigated as markers of atherosclerosis.…”