An enzyme-free and label-free fluorescent biosensor for small molecules by G-quadruplex based hybridization chain reaction

Chen, Qingai; Guo, Qingquan; Chen, Ying; Pang, Jie; Fu, FengFu; Guo, Liangqia

doi:10.1016/j.talanta.2015.02.002

Cited by 26 publications

(9 citation statements)

References 41 publications

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“…Detecting ATP and adenosine. The ATP sensor consists of NMM, a DNA strand composed of an ATP aptamer followed by a sequence complementary to a blocking probe, the blocking probe, and two DNA hairpins (H1 and H2), one of which can fold into a GQ [47]. This system uses an enzyme-free signal amplification strategy based on a hybridization chain reaction involving H1 and H2.…”

Section: Nmm-gq Complexes As Fluorescent Sensorsmentioning

confidence: 99%

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N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

Yett

Lin

Beseiso

et al. 2019

J. Porphyrins Phthalocyanines

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[Formula: see text]-methyl mesoporphyrin IX (NMM) is a water-soluble, non-symmetric porphyrin with excellent optical properties and unparalleled selectivity for G-quadruplex (GQ) DNA. G-quadruplexes are non-canonical DNA structures formed by guanine-rich sequences. They are implicated in genomic stability, longevity, and cancer. The ability of NMM to selectively recognize GQ structures makes it a valuable scaffold for designing novel GQ binders. In this review, we survey the literature describing the GQ-binding properties of NMM as well as its wide utility in chemistry and biology. We start with the discovery of the GQ-binding properties of NMM and the development of NMM-binding aptamers. We then discuss the optical properties of NMM, focusing on the light-switch effect — high fluorescence of NMM induced upon its binding to GQ DNA. Additionally, we examine the affinity and selectivity of NMM for GQs, as well as its ability to stabilize GQ structures and favor parallel GQ conformations. Furthermore, a portion of the review is dedicated to the applications of NMM-GQ complexes as biosensors for heavy metals, small molecules ([Formula: see text] ATP and pesticides), DNA, and proteins. Finally and importantly, we discuss the utility of NMM as a probe to investigate the roles of GQs in biological processes.

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Section: Nmm-gq Complexes As Fluorescent Sensorsmentioning

confidence: 99%

“…The specificity of the sensor is mostly defined by the specificity of the aptamer. Analysis of ATP in urine was successfully conducted using this NMM-GQ-based biosensor [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

Yett

Lin

Beseiso

et al. 2019

J. Porphyrins Phthalocyanines

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show abstract

“…Label-free fluorescent FNA biosensors are usually designed by using intercalating fluorescent dyes to intercalate FNA, and then changing the fluorescence response through the interaction of the target with FNA, the most common of which is the G-quadruplex structure of FNA [89]. Guanine nucleotides can form a tetrad structure through hydrogen bonds, and multiple such tetrad structures can be stacked to form a G-quadruplex structure [90].…”

Section: Fluorescent Functional Nucleic-acid Biosensorsmentioning

confidence: 99%

Recent Advances on Functional Nucleic-Acid Biosensors

Zhang

Guo

et al. 2021

Sensors

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In the past few decades, biosensors have been gradually developed for the rapid detection and monitoring of human diseases. Recently, functional nucleic-acid (FNA) biosensors have attracted the attention of scholars due to a series of advantages such as high stability and strong specificity, as well as the significant progress they have made in terms of biomedical applications. However, there are few reports that systematically and comprehensively summarize its working principles, classification and application. In this review, we primarily introduce functional modes of biosensors that combine functional nucleic acids with different signal output modes. In addition, the mechanisms of action of several media of the FNA biosensor are introduced. Finally, the practical application and existing problems of FNA sensors are discussed, and the future development directions and application prospects of functional nucleic acid sensors are prospected.

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“…The hemin/G‐quadruplex HRP‐mimicking DNAzyme catalyzes the H 2 O 2 ‐mediated oxidation (Li et al, ). This reaction can be detected by a colorimetrical signal using 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid; Hao et al, ; Lin et al, ) or 3,3′,5,5′‐Tetramethylbenzidine (TMB; Du et al, ; Wang et al, ), by chemiluminescence using luminol (Jiang et al, ; Li et al, ), and by fluorescence using N‐methyl mesoporphyrin IX (Chen et al, ; Sun et al, ) or Zinc protoporphyrin IX (Fu et al, ; Xue et al, ), respectively. Recently developed G‐quadruplex‐based HCR system was able to detect nucleic acid by constructing a hairpin set with a non‐split G‐quadruplex sequence in a closed stem and loop (Dong et al, ).…”

Section: Introductionmentioning

confidence: 99%

Colorimetric biosensor using dual‐amplification of enzyme‐free reaction through universal hybridization chain reaction system

Park

Rhee

Kim

et al. 2019

Biotech & Bioengineering

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On-site genetic detection needs to develop a sensitive and straightforward biosensor without special equipment, which can detect various genetic biomarkers. Hybridization chain reaction (HCR) amplifying signal isothermally could be considered as a good candidate for on-site detection. Here, we developed a novel genetic biosensor on the basis of enzyme-free dual-amplification of universal hybridization chain reaction (uHCR) and hemin/G-quadruplex horseradish peroxidase (HRP)-mimicking

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An enzyme-free and label-free fluorescent biosensor for small molecules by G-quadruplex based hybridization chain reaction

Cited by 26 publications

References 41 publications

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

N-methyl mesoporphyrin IX as a highly selective light-up probe for G-quadruplex DNA

Recent Advances on Functional Nucleic-Acid Biosensors

Colorimetric biosensor using dual‐amplification of enzyme‐free reaction through universal hybridization chain reaction system

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