An enzyme electrode for the amperometric determination of glucose

Guilbault, George G.; Lubrano, G. J.

doi:10.1016/s0003-2670(01)82476-4

Cited by 522 publications

(180 citation statements)

References 29 publications

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“…16 However, the process is time-consuming and requires two step of deposition for Ni and Au. Figure 3 shows cyclic voltammograms of the 3D-IDA and 2D-IDA electrodes in 0.5 M Na 2 SO 4 solution including 10 mM K 3 [Fe(CN) 6 ]. In this case, electrode length was narrowed to be 300 µm by pattering a photoresist on the electrodes, and Pt counter electrode was used.…”

Section: Resultsmentioning

confidence: 99%

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Fabrication of 3D Interdigitated Array Electrode Consisting of Au Nanoparticles and Its Application for Biosensing

et al. 2012

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We developed a 3D interdigitated (IDA) array electrode consisting of Au nano particles, which increase the electrode surface. The electrode was fabricated using microfabrication and printing techniques. Electrochemical impedance spectroscopy was conducted to measure IgG concentration, when anti-IgG was immobilized onto Dithiobis (succinimidyl propionate) (DTSP) SAM/3D-IDA electrode. A linear relationship between charge transfer resistance (R ct ) values and the logarithm of IgG concentration was observed in the range of 1 µg/ml to 1 mg/ml.

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Section: Resultsmentioning

confidence: 99%

“…In this case, Ag/AgCl was used as a reference electrode. EIS measurements were carried out using 700 µl of the PBS solution containing mixture of 5 mM Fe(CN) 6 4¹ and 5 mM Fe(CN) 6 3¹ for a redox probe. Change in charge transfer resistance (R ct ) at the electrode/electrolyte interface was investigated by electrochemical impedance.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of 3D Interdigitated Array Electrode Consisting of Au Nanoparticles and Its Application for Biosensing

et al. 2012

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“…It was a urea-sensor based on enzyme urease immobilized at an ammonium (NH4 + ) selective liquid membrane electrode (Guilbault and Montalvo 1970). In the year 1973, Guilbault and Lubrano described glucose and a lactate enzyme sensor based on hydrogen peroxide detection at a platinum electrode (Guilbault and Lubrano 1973). In 1974, Klaus Mosbach developed a heat-sensitive enzyme sensor termed 'thermistor' (Mosbach and Danielsson 1974).…”

Section: First Generation Enzyme Sensorsmentioning

confidence: 99%

Biosensors in food processing

2012

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Optical based sensing systems that measure luminescence, fluorescence, reflectance and absorbance, etc., are some of the areas of applications of optical immunosensors. Immunological methods rely on specific binding of an antibody (monoclonal, polyclonal or engineered) to an antigen. Detection of specific microorganisms and microbial toxins requires immobilization of specific antibodies onto a given transducer that can produce signal upon attachment of typical microbe/microbial toxins. Inherent features of immunosensors such as specificity, sensitivity, speed, ease and on-site analysis can be made use for various applications. Safety of food and environment has been a major concern of food technologists and health scientists in recent years. There exists a strong need for rapid and sensitive detection of different components of foods and beverages along with the food borne and water borne pathogens, toxins and pesticide residues with high specificity. Biosensors present attractive, efficient alternative techniques by providing quick and reliable performances. There is a very good potential for application of biosensors for monitoring food quality and safety in food and bioprocessing industries in India.

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“…The functional relationship is expressed in terms of an equivalent redox species bulk concentration, K: (10) The equivalent redox species bulk concentration, K, which captures effects of geometry and enzyme kinetics, depends upon the bulk concentrations of the analyte species and all cosubstrates, and the parameters listed in Table II. The derivation of Eq.…”

Section: Principlesmentioning

confidence: 99%

“…A biosensor is characterized by a biological recognition element in contact with the transducer surface. Enzymes are frequently chosen as the recognition element due to their remarkable specificity and inherent biocatalytic signal amplification [10][11][12]. Some biologically relevant analytes require a complex biosensing interface, with co-immobilization of multiple enzymes [5,13,14].…”

Section: Introductionmentioning

confidence: 99%

Theory of polymer entrapped enzyme ultramicroelectrodes: Fundamentals

Kottke

Kranz

Kwon

et al. 2008

Journal of Electroanalytical Chemistry

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We have developed a theoretical description of the amperometric response of ultramicroelectrode (UME) biosensors formed via enzyme entrapment. Our model allows for multiple enzymes and cosubstrates, and results in a closed-form analytical expression for the steady-state current response of the disk ultramicroelectrode. It captures the effects of enzyme-entrapment domain size, species transport properties (which can be different in the polymer and surrounding electrolyte), enzyme kinetics, and axisymmetric diffusion. Assumptions inherent in the derivation are carefully explained, as are the resulting limits on the applicability of the results. The ability to theoretically predict the response of enzyme entrapped UMEs should enable improved design, operation, and data interpretation for this important class of biosensors.

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An enzyme electrode for the amperometric determination of glucose

Cited by 522 publications

References 29 publications

Fabrication of 3D Interdigitated Array Electrode Consisting of Au Nanoparticles and Its Application for Biosensing

Fabrication of 3D Interdigitated Array Electrode Consisting of Au Nanoparticles and Its Application for Biosensing

Biosensors in food processing

Theory of polymer entrapped enzyme ultramicroelectrodes: Fundamentals

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