An enzyme containing microemulsion based on skin friendly oil and surfactant as decontamination medium for organo phosphates: Phase behavior, structure, and enzyme activity

Stehle, Ralf; Schulreich, Christoph; Wellert, Stefan; Gäb, Jürgen; Blum, Marc‐Michael; Kehe, Kai; Richardt, Andras; Lapp, Alain; Hellweg, Thomas

doi:10.1016/j.jcis.2013.09.027

Cited by 19 publications

(13 citation statements)

References 56 publications

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“…A low protein concentration is likely the underlying reason for a lack of change in T−S-derived parameters upon the incorporation of enzyme diisopropyl fluorophosphatase into an alkyl glucoside surfactant-based BμE system. 13 The incorporation of BSA, cytochrome c, and lysozyme also slightly increased S/V, consistent with the decrease in ξ 43 and ϕ mid ( Table 3), suggesting that the proteins promote the release of water and oil from the BμE phase to the W III excess phases. The effective monolayer thickness (t e ) for system 2 is 9−11 Å, similar to that for system 1 (Tables 2 and 3).…”

Section: Langmuirsupporting

confidence: 67%

Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study

Hayes

Rio

et al. 2015

Langmuir

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Small-angle neutron scattering (SANS) analysis using the Teubner-Strey model has been employed to evaluate the effect of protein incorporation into the middle, bicontinuous microemulsion (BμE) phase of Winsor-III (WIII) systems formed by an aerosol-OT (AOT)/alkyl ethoxylate mixed surfactant system to understand better the extraction of proteins into and out of BμEs and to study the effect of proteins on a system that serves as a biomimetic analog of cell membranes. Under conditions of high salinity, the incorporation of positively charged proteins cytochrome c, lysozyme, and α-chymotrypsin, near their solubilization limit in the BμEs promoted the release of water and oil from the BμEs, a decrease in the quasi-periodic repeat distance (d), an increase in ordering (a decrease in the amphiphilicity factor, fa) for the surfactant monolayers, and a decrease in the surface area per surfactant headgroup, suggesting that the proteins affected the self-assembly of components in the BμE phase and produced Debye shielding of AOT's sulfonate headgroup. For WIII systems possessing lower salinity, cytochrome c reduced the efficiency of surfactant in the BμE phase, noted by increases in d and fa, suggesting that the enzyme and AOT underwent ion pairing. The results of this study demonstrate the importance of ionic strength to modulate protein-surfactant interactions, which in turn will control the release of proteins encapsulated in the BμEs, relevant to WIII-based protein extraction and controlled release from BμE delivery systems, and demonstrate the utility of BμEs as a model system to understand the effect of proteins on biomembranes.

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Section: Langmuirsupporting

confidence: 67%

Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study

Hayes

Rio

et al. 2015

Langmuir

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“…Domestic and foreign scholars have begun to study environmental‐friendly decontaminant reagents, in order to degrade efficiently CWAs without damaging the environment. Among them, enzymatic decontamination and H 2 O 2 to neutralize HD have attracted much attention because of their environmental‐friendly, mild and efficient characteristics. Although enzymatic decontamination could degrade CWAs efficiently, enzymatic ones would be easily inactivated due to the influence of temperature, pH, solvent, ionic strength and even humidity.…”

Section: Introductionmentioning

confidence: 99%

Decontamination of the Mustard Gas (HD) Simulant CEES by Using a Molybdate‐Based ILs/H₂O₂ System

Li¹,

Xi²,

Wang³

et al. 2019

ChemistrySelect

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Due to the wide liquid range, good solubility, nonvolatility and designability, ionic liquids (ILs) have been considered as solvent in decontamination field and exhibited enormous potential. In this work, a series of 1‐alkyl‐3‐methylimidazolium molybdate ([CnMIm]2MoO4, n=4, 8, 12, 16) ILs are synthesized and characterized, which could improve the decontamination efficacy of mustard gas simulant 2‐chloroethylethylsulfide (CEES) in H2O2 through solubilization and catalysis. The practicability of functional ILs surfactant aqueous solution in decontamination field are analyzed by considering the influence factors about the critical micelle concentration (CMC), oxidant/CEES mole ratio, types and concentration of ILs, reaction kinetics and reaction activation energy. The results indicate that the decontamination efficacy of CEES is affected by both the concentration of MoO42− and the CMC of ILs. The decontamination efficacy against CEES in the [CnMIm]2MoO4/H2O2 system is accelerated by lengthening ILs cationic alkyl chain.

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“…The large interfacial area and the high solubility of substrate also favor the enzyme catalyzed conversion of hydrophobic substrates. 10,11 Yun et al studied the synthesis of n-hexyl-b-Dglucopyranoside catalyzed by b-glucosidase in the bicontinuous microemulsion consisting of tetradecane/water/CTAB/n-pentanol and found that the productivity in the bicontinuous microemulsion was much higher than that in a water-in-oil microemulsion. 3,5,6 In addition to reversed microemulsion, bicontinuous microemulsion is also a good medium for enzyme catalysis.…”

Section: Introductionmentioning

confidence: 99%

Study on the catalytic performance of laccase in the hydrophobic ionic liquid-based bicontinuous microemulsion stabilized by polyoxyethylene-type nonionic surfactants

Wang

et al. 2016

Soft Matter

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To formulate a compatible green medium for the conversion of a hydrophobic substrate by a hydrophilic enzyme, we investigated the phase behavior of pseudo ternary hydrophobic ionic liquid (HIL)/buffer/polyoxyethylene-type nonionic surfactant (CnEm)/n-alcohol system and the effects of the components on the formulation of the HIL-based bicontinuous microemulsion. It is found that small head group of the surfactant, high concentration of n-alcohol (medium/long alkyl chain) and low cohesive energy density of the HIL result in low phase transition temperature. In the CnEm stabilized compatible bicontinuous microemulsion, the kinetics of laccase catalyzed oxidation of 2,6-dimethoxyphenol were also investigated. It is found that in addition to temperature, n-alcohol is the key parameter affecting the catalytic performance of laccase, and the optimum n-alcohol depends on the type of HIL as an oil phase. All the kinetic parameters, such as Km, kcat, kcat/Km, and Ea (apparent activation energy), indicate that the bicontinuous microemulsion consisting of [Omim]NTf2/buffer/CnEm/n-hexanol is a suitable medium for the laccase-catalyzed reaction. To the best of our knowledge, this is the first report on the formulation of HIL-based bicontinuous microemulsion for enzyme catalysis.

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An enzyme containing microemulsion based on skin friendly oil and surfactant as decontamination medium for organo phosphates: Phase behavior, structure, and enzyme activity

Cited by 19 publications

References 56 publications

Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study

Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study

Decontamination of the Mustard Gas (HD) Simulant CEES by Using a Molybdate‐Based ILs/H₂O₂ System

Study on the catalytic performance of laccase in the hydrophobic ionic liquid-based bicontinuous microemulsion stabilized by polyoxyethylene-type nonionic surfactants

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An enzyme containing microemulsion based on skin friendly oil and surfactant as decontamination medium for organo phosphates: Phase behavior, structure, and enzyme activity

Cited by 19 publications

References 56 publications

Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study

Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study

Decontamination of the Mustard Gas (HD) Simulant CEES by Using a Molybdate‐Based ILs/H2O2 System

Study on the catalytic performance of laccase in the hydrophobic ionic liquid-based bicontinuous microemulsion stabilized by polyoxyethylene-type nonionic surfactants

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Product

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Decontamination of the Mustard Gas (HD) Simulant CEES by Using a Molybdate‐Based ILs/H₂O₂ System