An enzymatic glucose/O2 biofuel cell operating in human blood

Cadet, Marine; Gounel, Sébastien; Stinès-Chaumeil, Claire; Brilland, Xavier; Rouhana, Jad; Louërat, Frédéric; Mano, Nicolas

doi:10.1016/j.bios.2016.04.016

Cited by 110 publications

(66 citation statements)

References 53 publications

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“…GCE/Os(bpy) 2 PVI-LOx and GCE/ Os(bpy) 2 PVI-LOx/PAA were continuously operated at 0.2 V vs. SCE for 12 h in the presence of 35 mM lactate ( Figure S2). [20] The enhanced stability of GCE/Os(bpy) 2 PVI-LOx/PAA over that of GCE/Os(bpy) 2 PVI-LOx can be attributed to reduced levels of leakage of Os polymer and of the LOx, [20] and is indicative of the protective effects of the PAA coating. In contrast, with the PAA modified electrode G and the catalytic response decreased by 19 % and 42 %, respectively.…”

Section: Resultsmentioning

confidence: 99%

Use of Polymer Coatings to Enhance the Response of Redox‐Polymer‐Mediated Electrodes

et al. 2018

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The successful use of biosensors requires that the sensor can operate over abroad enough linear range that encompasses the physiological concentration of the substrate of interest. A polymer coating layer functioning as a mass-transport barrier is typically used to expand the linear range of biosensors with, however, the concomitant disadvantage of a reduction in the response. Effects of a poly(acrylic acid) coating layer on the response of a glassy carbon electrode modified with an Os redox polymer and lactate oxidase were evaluated. The coating layer resulted in an expanded linear range from 7 to 15 mM, doubled catalytic response towards the oxidation of 35 mM lactate, and improved operational stability. Detailed voltammetry studies revealed that the coating layer can improve the amount of the redox polymer that is available as a mediator, leading to the increased catalytic response at high concentrations of substrate. Similar results were obtained with other polymer layers [polystyrene sulfonate, poly(diallyldimethylammonium chloride), and poly(3,4-ethylenedioxythiophene)] and with the enzymes glucose oxidase and bilirubin oxidase, demonstrating the general nature of the method.[a] X.

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Section: Resultsmentioning

confidence: 99%

Use of Polymer Coatings to Enhance the Response of Redox‐Polymer‐Mediated Electrodes

et al. 2018

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“…GOx has potential uses in electrochemical applications, including biosensors104748 and biofuel cells444950515253545556. In addition, recent studies indicate that a good interface between GOx and CNTs is a critical factor in the efficient transfer of electrons from the GOx active site flavin to CNTs105758.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of enzyme-based coatings on intact multi-walled carbon nanotubes as highly effective electrodes in biofuel cells

Kim

Lee

Kwon

et al. 2017

Sci Rep

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CNTs need to be dispersed in aqueous solution for their successful use, and most methods to disperse CNTs rely on tedious and time-consuming acid-based oxidation. Here, we report the simple dispersion of intact multi-walled carbon nanotubes (CNTs) by adding them directly into an aqueous solution of glucose oxidase (GOx), resulting in simultaneous CNT dispersion and facile enzyme immobilization through sequential enzyme adsorption, precipitation, and crosslinking (EAPC). The EAPC achieved high enzyme loading and stability because of crosslinked enzyme coatings on intact CNTs, while obviating the chemical pretreatment that can seriously damage the electron conductivity of CNTs. EAPC-driven GOx activity was 4.5- and 11-times higher than those of covalently-attached GOx (CA) on acid-treated CNTs and simply-adsorbed GOx (ADS) on intact CNTs, respectively. EAPC showed no decrease of GOx activity for 270 days. EAPC was employed to prepare the enzyme anodes for biofuel cells, and the EAPC anode produced 7.5-times higher power output than the CA anode. Even with a higher amount of bound non-conductive enzymes, the EAPC anode showed 1.7-fold higher electron transfer rate than the CA anode. The EAPC on intact CNTs can improve enzyme loading and stability with key routes of improved electron transfer in various biosensing and bioelectronics devices.

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“…They have advantages as a wearable power source such as mild operating conditions and a simple device structure by eliminating a separator membrane. EBFCs can generate electricity on and in the living body using preloaded sugars or even biological fluid as fuel, and implantable applications of EBFCs to drive electric devices were also demonstrated . Nonetheless, the application of ionic current by EBFCs to wound healing on a living skin has never been reported.…”

mentioning

confidence: 99%

Accelerated Wound Healing on Skin by Electrical Stimulation with a Bioelectric Plaster

Kai

Yamauchi

Ogawa

et al. 2017

Adv Healthcare Materials

106

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Wound healing on skin involves cell migration and proliferation in response to endogenous electric current. External electrical stimulation by electrical equipment is used to promote these biological processes for the treatment of chronic wounds and ulcers. Miniaturization of the electrical stimulation device for wound healing on skin will make this technology more widely available. Using flexible enzymatic electrodes and stretchable hydrogel, a stretchable bioelectric plaster is fabricated with a built-in enzymatic biofuel cell (EBFC) that fits to skin and generates ionic current along the surface of the skin by enzymatic electrochemical reactions for more than 12 h. To investigate the efficacy of the fabricated bioelectric plaster, an artificial wound is made on the back skin of a live mouse and the wound healing is observed for 7 d in the presence and absence of the ionic current of the bioelectric plaster. The time course of the wound size as well as the hematoxylin and eosin staining of the skin section reveals that the ionic current of the plaster leads to faster and smoother wound healing. The present work demonstrates a proof of concept for the electrical manipulation of biological functions by EBFCs.

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An enzymatic glucose/O2 biofuel cell operating in human blood

Cited by 110 publications

References 53 publications

Use of Polymer Coatings to Enhance the Response of Redox‐Polymer‐Mediated Electrodes

Use of Polymer Coatings to Enhance the Response of Redox‐Polymer‐Mediated Electrodes

Fabrication of enzyme-based coatings on intact multi-walled carbon nanotubes as highly effective electrodes in biofuel cells

Accelerated Wound Healing on Skin by Electrical Stimulation with a Bioelectric Plaster

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