An EMS-induced mutation in a tetratricopeptide repeat-like superfamily protein gene (Ghir_A12G008870) on chromosome A12 is responsible for the liy short fiber phenotype in cotton

Fang, David D.; Naoumkina, Marina; Thyssen, Gregory N.; Bechere, Efrem; Li, Ping; Florane, Christopher B.

doi:10.1007/s00122-019-03456-4

Cited by 15 publications

(17 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cotton fiber length is largely determined at the elongation stage. As fiber length is very important to the quality of textiles, understanding the genetics and physiology of cotton fiber elongation can provide valuable tools to the cotton industry by targeting genes or cropping factors responsible for fiber elongation [3,4]. Cotton fiber mutants provide such a resource for the elucidation of fiber development mechanisms owing to the structural, morphological, and biochemical variances in the relevant fiber cells [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…There are numerous naturally occurred and human-made fiber mutants that display various fiber phenotypes [7]. These include, among others, fiberless mutant lines MD17, SL1-7-1, and XZ142w [8,9]; seeds with only lint fibers and no fuzz fibers in the naked seed lines N 1 [10] and n 2 [11]; and seeds that are described as extremely short lint fibers in the Ligon lintless-1 (Li 1 ) [12], Ligon lintless-2 (Li 2 ) [13], Li x [14] and li y [4] mutant lines. Among the four mutant lines, we selected three mutants, (i.e., Li 1 , Li 2 , and li y ) in this study.…”

Section: Introductionmentioning

confidence: 99%

“…A comparative study shows that at least seven of the 10 putative fiber development genes in the deletion region showed a higher expression in the wild type than in a Li 2 mutant during the fiber development stages. The li y mutant was created by treating the seeds of the cotton line MD15 with ethyl methanesulfonate [4,19]. A li y plant is short and stunted.…”

Section: Introductionmentioning

confidence: 99%

“…The li y gene locus is not allelic to either Li 1 or Li 2 [6]. A recent study indicates that a tetratricopeptide repeat-like superfamily protein gene (Ghir_A12G008870) on chromosome A12 is responsible for the li y short fiber phenotype [4]. Among the many genomic studies, there are limited reports which also measured selective fiber structural and quality parameters.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Surface and Thermal Characterization of Cotton Fibers of Phenotypes Differing in Fiber Length

Nam

Fang

et al. 2021

Polymers

Self Cite

View full text Add to dashboard Cite

Cotton is one of the most important and widely grown crops in the world. Understanding the synthesis mechanism of cotton fiber elongation can provide valuable tools to the cotton industry for improving cotton fiber yield and quality at the molecular level. In this work, the surface and thermal characteristics of cotton fiber samples collected from a wild type (WT) and three mutant lines (Li1, Li2-short, Li2-long, Li2-mix, and liy) were comparatively investigated. Microimaging revealed a general similarity trend of WT ≥ Li2-long ≈ Li2-mix > Li1 > Li2 short ≈ liy with Ca detected on the surface of the last two. Attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectroscopy and thermogravimetric measurements also showed that Li2-short and liy were more similar to each other, and Li2-long and Li2-mix closer to WT while Li1 was quite independent. FT-IR results further demonstrated that wax and amorphous cellulose were co-present in fiber structures during the fiber formation processes. The correlation analysis found that the FT-IR-based maturity parameter was well correlated (p ≤ 0.05) to the onset decomposition temperature and all three weight-loss parameters at onset, peak, and end decomposition stages, suggesting that the maturity degree is a better parameter than crystallinity index (CI) and other FT-IR parameters that reflect the thermal stability of the cotton fiber. In summary, this work demonstrated that genetic mutation altered the surface and thermal characteristics in the same way for Li2-short and liy, but with different mechanisms for the other three mutant cotton fiber samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Surface and Thermal Characterization of Cotton Fibers of Phenotypes Differing in Fiber Length

Nam

Fang

et al. 2021

Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…The chlorophyll ( Chl ) contents of leaves are positively correlated with photosynthetic efficiency and greatly affect the yield and quality of cotton [ 18 ]. Recently, EMS-induced mutation resulted in threonine/isoleucine substitution in a tetratricopeptide repeat-like superfamily protein which is responsible for the short fiber phenotype in Upland cotton [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Construction of Gossypium barbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

Abid

Wang

Zhu

et al. 2020

IJMS

View full text Add to dashboard Cite

Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton. Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library. At M6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

show abstract

Raman spectroscopic assessment of fibers and seeds of six cotton genotypes

Nam

Fang

2023

Agricultural & Env Letters

Self Cite

View full text Add to dashboard Cite

Raman spectroscopy (RS) is a vibrational spectroscopy. This work reported the RS spectral characteristics of fiber and seed of six cotton (Gossypium sp.) genotypes differing in fiber length. While the RS spectra of fiber samples were dominated by the cellulose-related peaks, the spectra of cottonseed samples were featured by the bands related to oil, protein, carbohydrate, and lignin components. Principal component analysis (PCA) revealed that the first two principal components (PCs) accounted for >87% of the total variation of the two types of samples. The PC1 versus PC2 plot classified the six fiber samples into three groups, but their cottonseeds into four groups. This experimental evidence implied the possibility of RS combined with PCA for rapid fiber phenotyping of cotton as well as for evaluating cottonseed nutrient information.

show abstract

An EMS-induced mutation in a tetratricopeptide repeat-like superfamily protein gene (Ghir_A12G008870) on chromosome A12 is responsible for the liy short fiber phenotype in cotton

Cited by 15 publications

References 58 publications

Surface and Thermal Characterization of Cotton Fibers of Phenotypes Differing in Fiber Length

Surface and Thermal Characterization of Cotton Fibers of Phenotypes Differing in Fiber Length

Construction of Gossypium barbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement

Raman spectroscopic assessment of fibers and seeds of six cotton genotypes

Contact Info

Product

Resources

About