An emerging function of circRNA-miRNAs-mRNA axis in human diseases

Ding, Rong; Sun, Handong; Li, Z.; Liu, Shuheng; Dong, Chaoxi; Fu, Kai; Tang, Weiwei; Cao, Hongxin

doi:10.18632/oncotarget.19154

Cited by 436 publications

(339 citation statements)

References 67 publications

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“…In recent years, circRNA-miRNA-gene regulatory axes were confirmed that involved in pathogenic processes or cancer [46,47]. In order to show a more intuitionistic result, three up-regulated circRNAs (chr5: 51521674-51524828-, chr3: 3137166-3138170+, chr4: 59505289-59510641-) in tumor liver samples of ALV-J-susceptible chickens were selected and five target miRNAs and 10 miRNA target genes were showed in the circRNA-miRNA-gene network which provides a foundation for further pathogenic mechanism research.…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

Zhang¹,

Yan²,

Lei³

et al. 2018

Oncotarget

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ALV-J (subgroup J avian leucosis virus) is a kind of oncogenic exogenousretrovirus and diseases associated with ALV-J have caused severe reproduction problems in the poultry industry worldwide. However, the pathogenesis of ALV-Jinduced tumor formation is still unclear. In recent years, circRNAs are validated to be related to cancer, which prompts us to explore whether aberrant expression of circRNAs exists in ALV-J-infected tumor samples. In this study, a total of 2822 differentially expressed circRNAs were detected by RNA sequencing in the tumor livers of ALV-J-susceptible chickens (n = 3) and the normal liver samples of ALV-J-resistant chickens (n = 3), among which 2808 circRNAs in the tumor liver samples were detected as unregulated compared to those in the normal liver samples. As circRNAs are considered as miRNA sponge, we predicted top 5 miRNA targets for 24 selected upregulated circRNAs in the ALV-J-induced tumor livers. CircRNA-miRNA-mRNA regulatory axes were also constructed to provide clear interaction relationship among them. Furthermore, Gene Ontology and KEGG pathway analyses were performed for miRNA target genes, the result of which implied that metabolism plays a key role in circRNAs alterations in ALV-J-induced tumor livers. In summary, we identified that circRNAs are involved in ALV-J-induced tumor formation in chickens. However, the pathogenicity mechanism still needs to be further explored.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

Zhang¹,

Yan²,

Lei³

et al. 2018

Oncotarget

View full text Add to dashboard Cite

show abstract

“…There is increasing evidence that circRNAs play important roles in the regulation of gene expression at the posttranscriptional level [5][6][7]. Accordingly, it has been demonstrated that many circRNAs serve as competing endogenous RNAs to bind with microRNAs (miRNAs) and inhibit the activity and function of the targeted miRNAs [8,9]. With the increasing number of studies on circRNAs, circRNAs have been shown to be involved in the development of several types of diseases, including cancer [10], cardiovascular disease [11], and neurological disorders [12].…”

Section: Introductionmentioning

confidence: 99%

Salivary Circular RNAs Hsa_Circ_0001874 and Hsa_Circ_0001971 as Novel Biomarkers for the Diagnosis of Oral Squamous Cell Carcinoma

Zhao

Wang

Ouyang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Recent studies have demonstrated that circular RNAs (circRNAs) can serve as potential molecular markers for disease diagnosis. However, little is known about their diagnostic potential for oral squamous cell carcinoma (OSCC). This study aimed to determine the expression of circRNAs in the saliva of OSCC patients to identify novel biomarkers for OSCC screening. Methods: Microarray screening of circRNA was performed to identify differentially expressed circRNAs in saliva from 3 OSCC patients compared with 3 healthy controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the results, and the association between these confirmed salivary circRNAs and clinicopathological features was analyzed using the chi-squared test. A receiver operating characteristic (ROC) curve was constructed to evaluate the diagnostic value of the circRNAs identified. Preoperative expression and postoperative expression (1 month after the surgery) of hsa_circ_0001874 and hsa_circ_0001971 was also determined. Results: Our results indicated 12 upregulated and 20 downregulated circRNAs in the saliva from the OSCC patients compared with that from the healthy controls. Among the differentially expressed circRNAs, hsa_circ_0001874, hsa_circ_0001971, and hsa_circ_0008068 were upregulated and hsa_circ_0000140, hsa_circ_0002632, and hsa_circ_0008792 were downregulated in the OSCC group versus the healthy group. Clinical data indicated that salivary hsa_circ_0001874 was correlated with TNM stage (P=0.006) and tumor grade (P=0.023) and that hsa_circ_0001971 was correlated with TNM stage (P=0.019). The combination of hsa_circ_0001874 and hsa_circ_0001971 showed an area under the ROC curve of 0.922 (95% confidence interval, 0.883-0.961; P< 0.001). The risk score based on the combination of hsa_circ_0001874 and hsa_circ_0001971 also discriminated patients with OSCC from patients with oral leukoplakia (P< 0.001). Moreover, the expression levels of salivary hsa_circ_0001874 and hsa_circ_0001971 were clearly decreased in the postoperative samples compared with preoperative samples (P< 0.001). Conclusions: This is the first study to demonstrate the potential of salivary hsa_circ_0001874 and hsa_circ_0001971 as biomarkers for the diagnosis of OSCC.

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“…For example, PCK1 and G6PC genes were annotated with the hepatic gluconeogenic related GO term: d-amino-acid oxidase activity (GO: 0003884). Previous study have found PCK1 and G6PC were the predominant genes that encode enzymes involved in the regulation of gluconeogenesis [55]. These results suggest that hepatic gluconeogenesis may be regulated by the influence of lncRNAs on these neighboring protein-coding genes.…”

Section: Discussionmentioning

confidence: 60%

The Landscape of Non-Coding RNA in an Adult Pig Model of Intrauterine Growth Restriction

Shen

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Intrauterine growth restriction (IUGR) is a risk factor for adult metabolic syndrome, but how this disease is regulated by lncRNAs and circRNAs remains elusive. Methods: Here, we employed adult IUGR and normal pigs as models to evaluate the expression of various global lncRNAs and circRNAs in pig livers using RNA-seq. Results: In total, we obtained 1,162 million raw reads of approximately 104.54 Gb high quality data. After a strict five-step filtering process, 3,368 lncRNAs were identified, including 300 differentially expressed lncRNAs (p < 0.05) in the IUGR group relative to the control group. The cis-regulatory analysis identified target genes that were enriched in specific GO terms and pathways (p < 0.05), including amino acid metabolism, oxidoreductase activity, PPAR signaling pathway, and insulin signaling pathway. These are closely related to the observed phenotypes of increased gluconeogenesis and impaired mitochondrial oxidative phosphorylation in adulthood of the IUGR group. Additionally, we also identified 403 circRNAs, of which 44 were differentially expressed (p < 0.05). Interestingly, our results identified ATF4-miR214-circRNA7964 and TCF7-miR22-3p-circRNA16347 as two competing endogenous networks, which were closely associated with the observed increase in hepatic gluconeogenesis in the IUGR group. Conclusion: Together, this study reveals a multitude of candidate lncRNAs and circRNAs involved in the development of IUGR pigs, which could facilitate further researches on the molecular mechanisms of metabolic syndrome.

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An emerging function of circRNA-miRNAs-mRNA axis in human diseases

Cited by 436 publications

References 67 publications

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

Salivary Circular RNAs Hsa_Circ_0001874 and Hsa_Circ_0001971 as Novel Biomarkers for the Diagnosis of Oral Squamous Cell Carcinoma

The Landscape of Non-Coding RNA in an Adult Pig Model of Intrauterine Growth Restriction

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