The Landscape of Non-Coding RNA in an Adult Pig Model of Intrauterine Growth Restriction

Shen, Linyuan; Zhang, Shunhua; Li, Qiang; Fu, Yuhua; Tang, Guping; Jiang, Yanzhi; Li, Mingzhou; Wang, Jinyong; Li, Xuewei; Che, Lianqiang; Zhu, Li

doi:10.1159/000494794

Cited by 10 publications

(10 citation statements)

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“…Accumulating evidence supports that circRNAs are important biological molecules associated with disease pathogenesis (Kristensen et al, 2019;Yu et al, 2019). Shen et al (2018) identified 44 differentially expressed circRNAs in pig livers using adult IUGR and normal pigs as models, via RNA sequencing. They identified ATF4/miR-214/circRNA7964 and TCF7/miR-22-3p/circRNA16347 as two competing endogenous networks by bioinformatic analysis (Shen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Shen et al (2018) identified 44 differentially expressed circRNAs in pig livers using adult IUGR and normal pigs as models, via RNA sequencing. They identified ATF4/miR-214/circRNA7964 and TCF7/miR-22-3p/circRNA16347 as two competing endogenous networks by bioinformatic analysis (Shen et al, 2018). In addition, circPAPPA and circTRNC18 play regulatory roles in trophoblast cells (Shen et al, 2019;Zhou et al, 2019) and a subset of placental cells, thus performing critical functions in fetal growth and survival.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA hsa_circ_0000848 Promotes Trophoblast Cell Migration and Invasion and Inhibits Cell Apoptosis by Sponging hsa-miR-6768-5p

Wang

Zhang

et al. 2020

Front. Cell Dev. Biol.

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Background: Fetal growth restriction (FGR) is a worldwide problem, and a major cause of perinatal morbidity. The precise molecular mechanisms involved in placental development and function during FGR remain poorly understood. Circular RNAs (circRNAs) are important biological molecules associated with disease pathogenesis. However, the role of circRNAs in FGR has not been well studied. Methods: circRNA expression profiles in placental tissues with and without FGR were identified by circRNA microarray. circRNA expression was verified by quantitative reverse-transcription PCR (RT-qPCR) assay. The effect of hsa_circ_0000848 and hsa-miR-6768-5p on HTR-8 cell apoptosis, migration, and invasion was evaluated. The association between hsa_circ_0000848 and hsa-miR-6768-5p was confirmed by dual luciferase activity and anti-AGO2 RNA immunoprecipitation (RIP) assays. Protein levels were examined via western blotting. Results: RT-qPCR results showed that hsa_circ_0000848 expression was significantly down-regulated in FGR placenta. Hsa_circ_0000848 overexpression and hsa-miR-6768-5p inhibitor suppressed apoptosis, and promoted cell migration and invasion. In addition, hsa_circ_0000848 overexpression and hsa-miR-6768-5p inhibitor increased the protein abundance of BCL2, MMP2 and MMP9, and decreased the protein abundance of cleaved caspase-3, cleaved caspase-9, and BAX, whereas hsa_circ_0000848 knockdown caused the opposite effect. Moreover, a significant increase in hsa-miR-6768-5p expression and a negative correlation between hsa_circ_0000848 and hsa-miR-6768-5p were identified in the FGR tissues. Luciferase reporter and RIP assay results revealed binding of hsa-miR-6768-5p to hsa_circ_0000848. Furthermore, hsa-miR-6768-5p overexpression eliminated the effect of hsa_circ_0000848 overexpression in HTR-8 cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0000848 Promotes Trophoblast Cell Migration and Invasion and Inhibits Cell Apoptosis by Sponging hsa-miR-6768-5p

Wang

Zhang

et al. 2020

Front. Cell Dev. Biol.

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“…A subsequent study found that LBW-induced changes in methylation in the intestine were mainly involved in biological processes, such as cell apoptosis and cell differentiation, suggesting that LBW piglets have a higher incidence of intestinal dysfunction (24). The adverse effects of LBW on fetuses and newborns may persist throughout the life cycle (25,26). Recent studies have shown that LBW young adults have a higher incidence of short-and long-term dysfunction in several vital organs (27)(28)(29); however, the effect of LBW on the intestinal barrier function of young adults is still not well characterized.…”

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confidence: 99%

Original low birth weight deteriorates the hindgut epithelial barrier function in pigs at the growing stage

Tao

Bai

et al. 2019

FASEB j.

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The deteriorative effect of low birth weight (LBW) on the mucosal permeability of the small intestine in piglets has been widely confirmed. However, whether the hindgut epithelial barrier function in LBW pigs is deteriorated during the growing stage is still unclear. Our study investigated differences in the hindgut epithelial barrier function between LBW and normal birth weight pigs during the growing stage (d 90). Our data demonstrated that the hindgut epithelium of LBW pigs has a high histopathological score, accompanied by decreased antioxidant capacity and increased apoptosis rate, as well as elevated expression and activity of caspase‐3. In addition, the number of intestinal goblet cells and gene expression of mucin 2 were significantly down‐regulated in LBW pigs. The expression of tight junction proteins (ZO‐1 and occludin) was markedly inhibited by the LBW. The mRNA abundance of inflammatory cytokines such as TNF‐α, IL‐1β, and IL‐8 was significantly increased in the hindgut mucosa of LBW pigs. Furthermore, our data revealed that LBW altered the intestinal microbial community in the hindgut mucosa of pigs. Collectively, these finding add to our understanding of the mechanisms responsible for hindgut epithelial barrier dysfunction in LBW pigs during the growing stage and facilitate the development of nutritional intervention strategies.—Tao, S., Bai, Y., Li, T., Li, N., Wang, J. Original low birth weight deteriorates the hindgut epithelial barrier function in pigs at the growing stage. FASEB J. 33, 9897–9912 (2019). http://www.fasebj.org

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“…According to our previous study [59], total RNA was extracted from PMM and LDM using TRIZOL (Invitrogen, Carlsbad, CA, USA), and further purified with RNeasy column (Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol. Total DNA was isolated from LDM and PMM for the measurement of mtDNA copy number by using the DNeasy Blood & Tissue Kit (Qiagen, USA).…”

Section: Methodsmentioning

confidence: 99%

“…As in our previous study [59], the clean data were obtained by removing reads containing adapters, reads containing over 10 % of poly (N), and low-quality reads (>50 % of the bases had Phred quality scores ≤ 10) from the raw data. All the downstream analyses were based on high quality clean data.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Analysis of lncRNAs and circRNAs Reveals the Metabolic Specialization in Oxidative and Glycolytic Skeletal Muscles

Shen

Gan

Tang

et al. 2019

IJMS

Self Cite

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The biochemical and functional differences between oxidative and glycolytic muscles could affect human muscle health and animal meat quality. However, present understanding of the epigenetic regulation with respect to lncRNAs and circRNAs is rudimentary. Here, porcine oxidative and glycolytic skeletal muscles, which were at the growth curve inflection point, were sampled to survey variant global expression of lncRNAs and circRNAs using RNA-seq. A total of 4046 lncRNAs were identified, including 911 differentially expressed lncRNAs (p < 0.05). The cis-regulatory analysis identified target genes that were enriched for specific GO terms and pathways (p < 0.05), including the oxidation-reduction process, glycolytic process, and fatty acid metabolic. All these were closely related to different phenotypes between oxidative and glycolytic muscles. Additionally, 810 circRNAs were identified, of which 137 were differentially expressed (p < 0.05). Interestingly, some circRNA-miRNA-mRNA networks were found, which were closely linked to muscle fiber-type switching and mitochondria biogenesis in muscles. Furthermore, 44.69%, 39.19%, and 54.01% of differentially expressed mRNAs, lncRNAs, and circRNAs respectively were significantly enriched in pig quantitative trait loci (QTL) regions for growth and meat quality traits. This study reveals a mass of candidate lncRNAs and circRNAs involved in muscle physiological functions, which may improve understanding of muscle metabolism and development from an epigenetic perspective.

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The Landscape of Non-Coding RNA in an Adult Pig Model of Intrauterine Growth Restriction

Cited by 10 publications

References 58 publications

Circular RNA hsa_circ_0000848 Promotes Trophoblast Cell Migration and Invasion and Inhibits Cell Apoptosis by Sponging hsa-miR-6768-5p

Circular RNA hsa_circ_0000848 Promotes Trophoblast Cell Migration and Invasion and Inhibits Cell Apoptosis by Sponging hsa-miR-6768-5p

Original low birth weight deteriorates the hindgut epithelial barrier function in pigs at the growing stage

Comprehensive Analysis of lncRNAs and circRNAs Reveals the Metabolic Specialization in Oxidative and Glycolytic Skeletal Muscles

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