An electrophoretic variant of 6-phosphogluconate dehydrogenase in rat and its application in detecting somatic cell hybrids

Preston, Sheryl L.; McMorris, F. Arthur

doi:10.1007/bf02395400

Cited by 12 publications

(2 citation statements)

References 13 publications

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“…The procedure used was as follows. Oneday-old rat pups of strain F344 or LEC (Preston and McMorris, 1983) were decapitated, and the brains were removed and placed in a small volume of saline 1 (McCarthy and de Vellis, 1978) adjusted to pH 7.2 with NaOH. Cerebral hemispheres were separated from the rest of the brain, meninges were removed, and the hemispheres were accumulated in a container of saline 1 until all were isolated.…”

mentioning

confidence: 99%

Cyclic AMP Induction of the Myelin Enzyme 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in Rat Oligodendrocytes

1983

Journal of Neurochemistry

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136

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Cyclic AMP (cAMP) is known to induce the activity of the myelin enzyme 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP; EC 3.1.4.37) in C6 rat glioma cells. This report shows that CNP is also inducible in oligodendrocytes explanted from 1-day-old rat cerebrum and grown in tissue culture. Induction was observed after a 1-day treatment with 1 mM N6, O2-dibutyryl cyclic AMP (dbcAMP) and was maximal after 5 days, reaching 200-240% of control. Induction was observed both in mixed cerebral cell cultures containing oligodendrocytes and astrocytes, and in purified cultures of oligodendrocytes prepared by a differential shakeoff procedure. Addition of dbcAMP to the cultures 3-9 days after the cells were explanted from rat brain induced CNP activity, but no induction was observed when dbcAMP treatment was begun 13 or more days after explanation. These results demonstrate that one component of myelin, CNP, is inducible in oligodendrocytes by a cAMP-mediated mechanism, and suggest a role for cAMP in the regulation of the myelin-associated functions of oligodendrocytes.

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mentioning

confidence: 99%

Cyclic AMP Induction of the Myelin Enzyme 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in Rat Oligodendrocytes

1983

Journal of Neurochemistry

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136

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“…At least 12 loci, Pr [4,9], Pa [3,17] , Db [4], Pmf [20], PmS [5,23], Ps [5] , G1 [39], CONI [7], CON2 [7], Pe [8], Po [8], and Pc [22] have been detected by PAGE or starch gel electrophoresis. On the other hand, in mice, these salivary protein loci, Ssp [21], Abp [12,13], and Msp-1 [32] have been found by polyacrylamide gel electrophoresis.…”

Section: Genetic Polymorphisms Of Salivary Proteinsmentioning

confidence: 99%

Studies on the Biochemical Marker Loci Detected with Urine, Tears, and Saliva of Rats

Kondo

1994

Experimental Animals

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Insulin‐like growth factor I promotes cell proliferation and oligodendroglial commitment in rat glial progenitor cells developing in vitro

McMorris

Dubois‐Dalcq

1988

J of Neuroscience Research

351

179

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We investigated the mechanisms by which insulin-like growth factor I (IGF-I) acts to increase the number of oligodendrocytes that develop in cultures of cells explanted from perinatal rat cerebrum. Fluorescence-activated cell sorting was used to isolate bipotential A2B5-positive oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells, which were then inoculated as single cells into microculture wells containing feeder layers of X-irradiated type 1 astrocytes. Addition of 100 ng/ml IGF-I to the culture medium increased the growth rate and the ultimate size reached by the resulting clones during the 18-day experimental period. Moreover, 75-80% of the cells in the IGF-I-treated clones differentiated into galactocerebroside (GC)-positive oligodendrocytes, whereas only 25-30% became oligodendrocytes in the absence of IGF-I. IGF-I did not increase the number of type 2 astrocytes that developed in the clones. IGF-I appeared to have the greatest effect on growth and differentiation at a stage when the majority of the cells in the clones were at an intermediate stage of development, characterized by the expression of A2B5 and O4 glycolipid antigens but not GC. Analysis of the effects of IGF-I on O4-positive, GC-negative intermediate precursor cells revealed a two to fivefold increase in the number of cells that incorporated 3H-thymidine into their DNA during a 5-h pulse. Moreover, IGF-I increased the number of cell sorter-purified O4-positive cells that developed into oligodendrocytes 4-8 days later. Therefore, IGF-I acts in two different ways to promote oligodendrocyte development: It promotes proliferation of precursor cells in the O-2A lineage, and it induces precursors to become committed to develop into oligodendrocytes.

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An electrophoretic variant of 6-phosphogluconate dehydrogenase in rat and its application in detecting somatic cell hybrids

Cited by 12 publications

References 13 publications

Cyclic AMP Induction of the Myelin Enzyme 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in Rat Oligodendrocytes

Cyclic AMP Induction of the Myelin Enzyme 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in Rat Oligodendrocytes

Studies on the Biochemical Marker Loci Detected with Urine, Tears, and Saliva of Rats

Insulin‐like growth factor I promotes cell proliferation and oligodendroglial commitment in rat glial progenitor cells developing in vitro

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