An Electrophoretic Analysis of Schistosoma mansoni Soluble Egg Antigen Preparation

Carter, Clint E.; Colley, Daniel G.

doi:10.2307/3279763

Cited by 60 publications

(30 citation statements)

References 13 publications

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“…Soluble SEA preparation (4,8) was coupled to CNBr-activated Sepharose 4B (Sigma Chemical Co., St. Louis, MO) (9), and used to affinity purify multiclonal anti-SEA antibodies from pooled or individual sera (9). Eluates obtained by use of 0.1 M glycine-HCI (pH 2.8) were neutralized with 0.1 M NaOH, dialyzed against 0.05 M ammonium bicarbonate, lyophilized, redissolved in phosphatebuffered saline; sterilized by filtration, and their protein content determined (10).…”

Section: Methodsmentioning

confidence: 99%

Immune responses during human Schistosomiasis mansoni. Evidence for antiidiotypic T lymphocyte responsiveness.

Lima¹,

Gazzinelli²,

Nascimento³

et al. 1986

J. Clin. Invest.

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We present a method for the examination of antiidiotypic cellmediated reactivity during chronic human infections. Pooled and individual sera from patients with schistosomiasis. mansoni were purified on immunoaffinity columns of schistosomal egg antigens (SEA). The eluates contained anti-SEA antibodies, but not SEA. These antibody preparations, and their F(ab')2 fragments, stimulated dose-dependent proliferation of peripheral blood mononuclear cells (PBMN) and T lymphocytes from some, but not all active or former schistosomiasis mansoni patients, and could do so autologously. Stimulation required presentation by plasticadherent cells. The eluates did not stimulate PBMN from persons who had never had schistosomiasis. Affinity-purified anti-SEA antibodies from former patients (cured for >10 yr) did not stimulate PBMN from patients with active infections. Reabsorption on SEA columns removed stimulatory activity from the eluates. We propose that multiclonal, SEA-related idiotypes expressed by some anti-SEA antibodies stimulate proliferation of T lymphocytes that express antiidiotypic specificities.

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Section: Methodsmentioning

confidence: 99%

Immune responses during human Schistosomiasis mansoni. Evidence for antiidiotypic T lymphocyte responsiveness.

Lima¹,

Gazzinelli²,

Nascimento³

et al. 1986

J. Clin. Invest.

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“…A total of 100 l of cell suspension was added to a 96-well plate containing soluble egg antigen (SEA) at a final concentration of 50 g/ml or only culture medium. Soluble egg antigen was prepared from livers of S. mansoni-infected mice, as previously described (5). Spleen cells plated as described above were maintained for 48 h in a humidified cell incubator in the presence of air containing 5% CO 2 .…”

Section: Animals and Infectionmentioning

confidence: 99%

Experimental Infection withSchistosoma mansoniin CCR5-Deficient Mice Is Associated with Increased Disease Severity, as CCR5 Plays a Role in Controlling Granulomatous Inflammation

Souza

Pereira

et al. 2011

Infect Immun

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The plasma level of the chemokine CCL3 is elevated in patients with chronic severe schistosomiasis mansoni. We have previously shown that CCL3؊/؊ mice with experimental infection showed diminished pathology and worm burden compared to those of wild-type (WT) mice. To elucidate further the role of CC chemokines during schistosomiasis mansoni infection, we evaluated the course of infection in C57BL/6J mice deficient in CCR5, one of the receptors for CCL3. The CCR5 deficiency proved to be remarkably deleterious to the host, since mortality rates reached 70% at 14 weeks postinfection in CCR5 ؊/؊ mice and 19% in WT mice. The increased lethality was not associated with an increased parasite burden, since similar numbers of eggs and adult worms were found in mice from both groups. Liver granulomas of chronically infected CCR5 ؊/؊ mice were larger and showed greater numbers of cells and collagen deposition than liver granulomas from WT mice. This was associated with higher levels of production of intereleukin-5 (IL-5), IL-13, CCL3, and CCL5 in infected CCR5 ؊/؊ mice than in infected WT mice. Moreover, at 8 weeks after infection, just before changes in pathology and mortality, the numbers of FoxP3-positive cells were lower in liver granulomas of CCR5 ؊/؊ mice than in WT mice. In conclusion, the CCR5 deletion is deleterious to mice infected with Schistosoma mansoni, and this is associated with enhanced fibrosis and granulomatous inflammation.

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“…The methods of preparation of the saline extracts SEA from S. mansoni eggs (22,23) and EPI from T. cruzi epimastigotes ( 17,24) has been detailed elsewhere. Likewise, the affinity purification of anti-SEA (15) and anti-EPI (17) antibodies on columns ofSEA or EPI coupled to Sepharose 4B (Sigma Chemical Co., St. Louis, MO) from the sera of patients with schistosomiasis or Chagas' disease, respectively, has been presented.…”

Section: Introductionmentioning

confidence: 99%

Idiotypic sensitization in utero of children born to mothers with schistosomiasis or Chagas' disease.

Elói-Santos

Novato-Silva²,

Maselli³

et al. 1989

J. Clin. Invest.

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Cord blood mononuclear cells (CBMC) of neonates born of mothers with Chagas' disease or schistosomiasis exhibited strong proliferative responses against idiotypes expressed on antibodies with specificity for Trypanosoma cruzi or Schistosoma mansoni antigens, respectively. These immunoaffinitypurified preparations were stimulatory if they were prepared from pools of patients' sera or from the mother's own serum, taken early during her pregnancy. These CBMC did not respond to normal immunoglobulin, and CBMC of neonates born of uninfected mothers did not respond to antibodies against either T. cruzi or S. mansoni, or normal immunoglobulin preparations.We propose that in utero exposure of a fetus to some idiotypes expressed on placentally transferred antibodies induces anti-Id T lymphocyte sensitization, which we detect as a proliferative response by CBMC exposed to immunoaffinity-purified antibodies expressing the relevant idiotypes. This is the first experimental evidence that children born of mothers with chronic infections undergo natural in utero idiotypic manipulations and are born possessing cellular anti-Id reactivity.

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An Electrophoretic Analysis of Schistosoma mansoni Soluble Egg Antigen Preparation

Cited by 60 publications

References 13 publications

Immune responses during human Schistosomiasis mansoni. Evidence for antiidiotypic T lymphocyte responsiveness.

Immune responses during human Schistosomiasis mansoni. Evidence for antiidiotypic T lymphocyte responsiveness.

Experimental Infection withSchistosoma mansoniin CCR5-Deficient Mice Is Associated with Increased Disease Severity, as CCR5 Plays a Role in Controlling Granulomatous Inflammation

Idiotypic sensitization in utero of children born to mothers with schistosomiasis or Chagas' disease.

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