Idiotypic sensitization in utero of children born to mothers with schistosomiasis or Chagas' disease.

Elói-Santos, Silvana Maria; Novato-Silva, Eliane; Maselli, V M; Gazzinelli, Giovanni; Colley, Daniel G.; Correa-Oliveira, Rodrigo

doi:10.1172/jci114225

Cited by 64 publications

(42 citation statements)

References 26 publications

(15 reference statements)

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“…Idiotypic sensitization in utero has also been demonstrated in children born to mothers with chronic parasitic infections. These children were born with T lymphocyte-mediated antiidiotype reactivity, a priming that might be important if they became infected (15). The fetus can produce specific antibodies against antigens to which the mother has been vaccinated during pregnancy as well (16,17).…”

Section: Discussionmentioning

confidence: 99%

Presence of Non-Maternal Antibodies in Newborns of Mothers with Antibody Deficiencies

et al. 1992

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Samples from four hypogammaglobulinemic moth ers and their newborns as well as two Ig/v-deficient moth ers and their neonates were studi ed. Mother I whose first infant was investigated earlier (1), now had her second baby investigated. From mother II samples from two pregnancies were included. At delivery, we tried to obtain am niotic fluid, cord serum , and mother's serum. Cord blood was taken from the placental side of the umbilical cord. All samples were frozen at -20ce . Du ring the first few days postpartum, mother's and infant's saliva, meconium, and maternal milk (not included in this study) were collected. Samples were frozen immediately at -20°C. The babies had not been given either the mother's or bank milk before the sample was taken. Th e time passed from the last feeding was recorded, as was the mode of feeding. Th e saliva was obtai ned as unstimulated whole saliva with a l-mL syringe or a rounded eye-drop pipette during 15 min and was immediately frozen at -20°C.The hypogamm aglobulinem ic mothers were on proph ylaxis with ' 16.5% Ig intramuscularly (Gammaglobulin; KabiPharmacia, Stockholm, Sweden), a preparation shown to contain idiotypic and antiidiotypic antibod ies to poliovirus type I (HahnZoric M, Carlsson B, Jeansson S, Ekre HP , Osterhaus ADME, Roberton D, Hanson LA., unpubl ished data).Preparation ofmeconium extracts. Previously freeze-dried meconium samples were weighed, and 0.1 g of dry material was dissolved in 3 mL of PBS, pH 7.2, in centrifuge tub es with round bottoms. Glass beads were added to each test tub e and the mixture was shaken on a whirl-mixer six times du ring a 30-min interval with a 5-min pause in between at 4°C. After that, the samples were centrifuged for 20 min at 1500 x g and the clear supernatant was used immediately for analysis. Tween 20 (Merck, Darmstadt, Germany) was added in a concentration of 0.05% to the extracts that were analyzed undiluted. Antibody determ inations. Total maternal serum IgG, IgM, andIgA levels were quantified by radial immunodiffusion (2). 19A 150 ABSTRACT. To explain the mechanism for induction and production of specific antibodies found in the newborn alread y at birth, without previous known exposure to the antigen, we chose a model that presumably excluded the possibility of specific antibodies being transferred from the mother to the fetus. Specific IgG, IgA, and IgM antibodie s against Escherichia coli and poliovirus antigens were determined with ELISA in serum, saliva, and amniotic fluid from hypogammaglobulinemic and IgA-deficient mothers as well as in cord serum, sali va, and meconium from their offspring. All the mothers lacked IgA and some also lacked IgM antibodies , which were found in their healthy newborns. The amniotic fluid from a hypogammaglobulinemic mother lacking IgA contained small amounts of Igaantibodies, which were also found in the neonate, suggesting a fetal origin. There was evidence for the presence of antiidiotypic antibodies to poliovirus in the cord sera. We propose that idiotypic and/or antiidiot ypic IgG ...

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Section: Discussionmentioning

confidence: 99%

Presence of Non-Maternal Antibodies in Newborns of Mothers with Antibody Deficiencies

et al. 1992

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“…Most humans exposed to schistosomiasis are born to mothers who are or have been infected. There are ample reasons to think that in utero exposure to schistosome antigens or to idiotypic or anti-idiotypic antibodies may modify the response to subsequent infection in humans (Eloi-Santos et al 1989, Novato-Silva et al 1992) and in mice (Montesano et. al.…”

Section: General Considerations In the Interpretation Of Models Of Scmentioning

confidence: 99%

Experimental models of Schistosoma mansoni infection

Cheever

Lenzi²,

Lenzi³

et al. 2002

Mem. Inst. Oswaldo Cruz

120

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“…13 Exposure to maternal antigens might predispose a child to develop an immunoregulated, asymptomatic clinical presentation of the infection, and may predispose them to respond with an altered, perhaps more immunoregulated, form of schistosomiasis. 4,5,14 The probably immunogenic effects of such antigens as vaccines among infected mothers and the proper time to vaccinate newborns need to be studied.…”

Section: Discussionmentioning

confidence: 99%

“…3 Exposure to maternal schistosome antigens might also predispose a child to respond with an altered, perhaps more immunoregulated form of schistosomiasis. 4,5 Thus, defining the extent to which maternal schistosome antigens may or not interfere with responses to new infant vaccines requires a better understanding of its main determinants.…”

Section: Introductionmentioning

confidence: 99%

Placental and Oral Delivery of Schistosoma Mansoni Antigen From Infected Mothers to Their Newborns and Children

Attallah

Ghanem

Ismail

et al. 2003

The American Journal of Tropical Medicine and Hygiene

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Abstract. A 63-kD Schistosoma mansoni antigen was detected in 149 (86%) of 174 umbilical cord blood sera from infected women at delivery. Specific IgG antibodies to this antigen were also detected in 80% of cord blood sera. The 63-kD antigen showed the same molecular mass by Western blotting when isolated from cord blood, maternal blood, breast milk, and urine from women infected with S. mansoni. This antigen was detected in the urine of 25 infants born to infected mothers and followed for 18-24 months after delivery. It was also detected in some infants up to 21 days after parturition and then disappeared at 28 days, demonstrating the influence of breast-feeding on persistence of antigen in infants born to infected women. Thus, exposure to Schistosoma antigens and maternal antibodies to this organism may influence the developing immune responses to natural infection or vaccination of children born in endemic areas.

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Idiotypic sensitization in utero of children born to mothers with schistosomiasis or Chagas' disease.

Cited by 64 publications

References 26 publications

Presence of Non-Maternal Antibodies in Newborns of Mothers with Antibody Deficiencies

Presence of Non-Maternal Antibodies in Newborns of Mothers with Antibody Deficiencies

Experimental models of Schistosoma mansoni infection

Placental and Oral Delivery of Schistosoma Mansoni Antigen From Infected Mothers to Their Newborns and Children

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