An Electron Microscope Study of the Embryology of the Intercalated Disc in the Heart of the Rabbit

Muir, A L

doi:10.1083/jcb.3.2.193

Cited by 112 publications

(38 citation statements)

References 9 publications

(8 reference statements)

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“…4). The blocks of thick filaments probably correspond to the "Purkinje fibrils" described by MUIR (1957) in the sheep heart. This aspect will be described again later.…”

Section: Observationsmentioning

confidence: 99%

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Fine Structure of Purkinje Fibers in the Chick Heart

Hirakow

1966

Arch. Histol. Jap., Arch. Histol. Jpn

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“…4). The blocks of thick filaments probably correspond to the "Purkinje fibrils" described by MUIR (1957) in the sheep heart. This aspect will be described again later.…”

Section: Observationsmentioning

confidence: 99%

“…There have been a considerable number of electron microscope studies on the conducting tissue of the mammalian heart (CAESAR et al 1958, HAYASHI 1962, KAWAMURA 1961, MUIR 1957, RHODIN et al 1961, TORII 1962, and VIRAGH et PORTE 1961. In birds, however, this tissue has never been observed at the submicroscopic level, as far as the author knows.…”

mentioning

confidence: 99%

Fine Structure of Purkinje Fibers in the Chick Heart

Hirakow

1966

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Muir (1957) has shown that the intercalated discs are important for cardio-myofibrillogenesis. The irregular small striations labeled with the antibody against α-actinin in the transfected cardiomyocytes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Suppression of Cardiac Troponin T Induces Reduction of Contractility and Structural Disorganization in Chicken Cardiomyocytes

Toyota

Takano‐Ohmuro

Yoshida

et al. 2008

Cell Struct. Funct.

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ABSTRACT. We herein examine the effect of cardiac troponin T (CTnT) suppression in cultured chicken cardiomyocytes derived from embryonic cardiac ventricular muscle. TnT is an important protein participating in regulation of striated muscle contraction, but it is not clear whether TnT contributes to the formation of sarcomere structure in myofibrils. Double-stranded RNA homologous to the nucleotide sequence of CTnT (CTnTsiRNA) was introduced into cultured muscle cells two days after plating. Transfection efficiency was above 80%. Immunoblot analyses suggested that the expression of CTnT progressively falls for the three consecutive days after transfection, but partly reappears on the fourth day. Maximum suppression occurs three days after transfection, with almost invisible CTnT protein on immunoblots in all the examined conditions: 0.5-2 nmol CTnT-siRNA towards 1-3×10 6 cells. The suppression was specific to CTnT, and the other myofibrillar proteins such as myosin, connectin/titin, tropomyosin, alpha-actinin, and troponin I were all present in transfected cells. The following functional and morphological changes were detected in CTnT-suppressed cells. The population of beating cells decreased significantly after transfection, when compared to control cells. A part of CTnTsuppressed cells showed two non-overlapping types of morphological changes: 1) myofibrils presenting unusually long Z-Z intervals; 2) myofibrils with irregular small striations in cells not connected at their adhesion interfaces of a jagged-appearance. Thus, our results reveal that CTnT is important for stable beating in cultured ventricular muscle cells, and also to some extent, for maintaining myofibrillar structure and cell-to-cell adhesion.

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“…The complexity and number of junctions increases postpartum until juvenile life (3,23). Perhaps this is the basis of the relative difficulty of obtaining robust myocardial cells from adult donors.…”

Section: Discussionmentioning

confidence: 99%