Culture of Spontaneously Contracting Myocardial Cells from Adult Rats

Jacobson, Stuart L.

doi:10.1247/csf.2.1

Cited by 85 publications

(21 citation statements)

References 27 publications

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“…The growth of neonatal and adult mammalian ventricular cardiac muscle cells in long-term culture (Jacobson, 1977;Claycomb and Palazzo, 1980;Claycomb and Lanson, 1984) and the effect of various extracellular matrix substrates (ECM) on their morphologies have been the subject of several recent studies Lundgren et al, 1984Lundgren et al, , 1985Lundgren et al, , 1988Cooper et al, 1986). These studies have demonstrated that adult and neonatal cardiac myocytes attach and spread differently when cultured on a larninin-coated substratum compared to similar myocytes grown on plastic.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural morphometric analysis of cultured neonatal and adult rat ventricular cardiac muscle cells

1989

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Neonatal and adult rat ventricular cardiac muscle cells cultured on laminin differed from similar myocytes grown on plastic in the amount and distribution of their mitochondria and transverse tubules. Point-count morphometry was used at the electron microscopic level to quantify these differences. Adult myocytes grown on laminin contained more mitochondria per unit volume than adult myocytes grown on plastic. No significant differences were observed in the volume percent of myofibrils in either adult or neonatal ventricular myocytes when grown on laminin and compared to those grown on plastic. The transverse tubule system in neonatal and adult myocytes was reduced significantly when both groups were cultured on laminin. Furthermore, neonatal and adult myocytes cultured on laminin were flatter than those cultured on plastic. This may indicate a relationship between the surface/volume ratio and transverse tubule development in cultured myocytes. These studies establish that point-count morphometry can be used to quantify changes in the organelle volume densities of cultured cardiac muscle cells.

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Section: Introductionmentioning

confidence: 99%

Ultrastructural morphometric analysis of cultured neonatal and adult rat ventricular cardiac muscle cells

1989

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“…Another very puzzling phenomenon is the behavior of adult myocytes in culture. These must first round up prior to attachment (Jacobson, 1977), and Claycomb and Pallazo (1980) have reported that the rounded form is unable to exclude trypan blue. Yet, after days or weeks, depending on the age of the donor animals, cells flatten, send out projections, and begin to beat spontaneously.…”

Section: Discussionmentioning

confidence: 99%

Release of enzymes from adult rat heart myocytes.

Murphy¹,

Hohl²,

Brierley³

et al. 1982

Circulation Research

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SUMMARY. The release of lactic dehydrogenase, creatine phosphokinase, and aspartate aminotransferase from initially viable, metabolically competent adult rat heart myocytes has been examined. Freshly isolated cells contain levels of total lactic dehydrogenase, creatine phosphokinase, and aspartate aminotransferase, as well as lactic dehydrogenase and creatine phosphokinase isoenzyme profiles that are quite comparable to those of intact heart tissue. When the cells are lysed with digitonin, 89% of total lactic dehydrogenase, but only 58% of creatine phosphokinase and 27% of aspartate aminotransferase are released. The retention of creatine phosphokinase by the digitoninlysed cells is accounted for by complete retention of mitochondrial creatine phosphokinase and 20% of MM-creatine phosphokinase. When intact myocytes are incubated under anoxic, substratedeprived conditions, there is a gradual loss of the three enzymes to the suspending medium and a parallel increase in the fraction of the cells permeable to trypan blue. The fraction of freely soluble cytoplasmic enzymes lost was equivalent to the fraction of the cells permeable to the dye over a wide range of viability (17-95% viable by dye exclusion criteria), but permeable cells retained mitochondrial creatine phosphokinase and particulate aspartate aminotransferase. These results suggest that simultaneous and complete release of soluble cytoplasmic enzymes occurs as each individual cell sustains sarcolemmal damage.

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“…Cavanaugh also noted that these cells could divide and migrate away from the other cells in the nutrient medium. Later, contractile activity modifiable by cardiac drugs was observed in isolated embryonic rat heart cells in vitro [8] and in cells from adult rat hearts [9].…”

Section: Brief Historical Overview Of the Attempts To Cultivate Rhythmentioning

confidence: 99%

We heart cultured hearts. A comparative review of methodologies for targeted differentiation and maintenance of cardiomyocytes derived from pluripotent and multipotent stem cells

Arabadjiev¹,

Petkova²,

Chakarov³

et al. 2014

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Citation: Arabadjiev A, Petkova R, Chakarov S, Pankov R, Zhelev N. We heart cultured hearts. A comparative review of methodologies for targeted differentiation and maintenance of cardiomyocytes derived from pluripotent and multipotent stem cells. AbstractHuman cell lines, including disease cell lines are often superior to routine animal models for the purposes of rapid and safe assessment of the effects of different agents that may modulate myocardial functioning under physiological and pathological conditions. There are several currently existing methodologies for derivation of cardiomyocyte-like cells by targeted differentiation from pluripotent cells and by reprogramming/ transdifferentiation from other types of cells (multipotent progenitors, somatic cells, etc). The present paper reviews the potential sources of cells capable of differentiation along the cardiomyocyte lineage; the existing methodologies for targeted differentiation, outlining the specificities of each method; and the markers for differentiation along the mesodermal and the cardiogenic lineage. The yield of robustly beating cells expressing cardio-specific proteins derived by any of the existing methods, however, still rarely exceeds 70-90 %, even with the newly developed approaches for increasing the differentiation capacity. There still is significant variance in the results obtained by different research groups and even between different experiments carried out in the same laboratory, with the same type of cells and same general type of protocol. Derivation of new lines of human pluripotent and multipotent stem cells according to standardised protocols and in completely defined; xeno-free conditions may increase the reliability and reproducibility of research and speed up the development of potential clinical applications.

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Culture of Spontaneously Contracting Myocardial Cells from Adult Rats

Cited by 85 publications

References 27 publications

Ultrastructural morphometric analysis of cultured neonatal and adult rat ventricular cardiac muscle cells

Ultrastructural morphometric analysis of cultured neonatal and adult rat ventricular cardiac muscle cells

Release of enzymes from adult rat heart myocytes.

We heart cultured hearts. A comparative review of methodologies for targeted differentiation and maintenance of cardiomyocytes derived from pluripotent and multipotent stem cells

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