An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1

Abstract

The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection of E. coli O157:H7 and Vibrio cholerae O1. This approach was based on the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification. By employing the proposed biosensor, the concentrations of these pathogenic bacteria could be established on a single interface in a single run with improved sensitivity and accuracy. The successful approach of the simultaneous detection and quantification of two bacteria by an electrochemical biosensor demonstrated here could be readily expanded for the estimation of a variety of other pathogenic bacteria, proteins, and nucleotides. Because of their high sensitivity, electrochemical biosensors may represent a new avenue for early diagnosis of diseases.

Ultrasensitive Bioaffinity Electrochemical Sensors: Advances and New Perspectives

“…In recent years, molecularly imprinted polymers (MIPs) have been used as promising alternatives to natural receptors to developing new generation of chemical/biological sensors due to their superiorities such as high adsorption capacity, high specific recognition of target molecules, desirable physical and chemical stability, excellent reusability, low cost and easy preparation offers. Reproduced from [109] with permission from Ivyspring International Publisher (E. coli O157 : H7 and Vibrio colerae = 1). Electrochemical sensors, provide much lower cost, more field portability and are predominant device in the market [114].…”

Molecular Biosensors for Electrochemical Detection of Infectious Pathogens in Liquid Biopsies: Current Trends and Challenges

“…At this point, it is worth to mention that very recently the Lobo-Castañón’s research group has reported, for the first time, the use of on-surface helicase-dependent amplification at ITO surfaces to quantify electrochemically very small amounts of genomic DNA extracted from Salmonella [ 31 ]. Attractive electrochemical nucleic acid sensors have been reported so far for non-invasive bacterial infections detection coupled with PCR [ 32 , 33 , 34 ], HDA [ 25 , 31 , 34 ], RPA [ 35 ] and HCR [ 36 ].…”

“…Electrochemical biosensors described to date for bacterial infections [ 28 , 32 , 33 , 34 , 35 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ] comprise a rather limited number of immunosensors using antibodies able to detect the whole bacteria ( Streptococcus pseudopneumoniae , Escherichia coli and Vibrio cholerae ) [ 36 , 37 , 38 ] or specific surface bacterial proteins (PspA peptide) [ 28 ], and a wide variety of nucleic acid sensors targeting specific bacterial genes used along with different nucleic acid and signal amplification strategies [ 32 , 33 , 34 , 35 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ].…”

“…Li et al [ 36 ] developed an electrochemical strategy using multifunctional nanoconjugates for sensitive simultaneous detection of Escherichia coli O157:H7 and Vibrio cholerae O1. The method utilized the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification.…”

“…Abbreviations: Ab(O157) or Ab(O1), antibodies for Escherichia coli O157:H7 or Vibrio cholerae O1; Bio-Ab, biotinylated antibodies; H1–4, hairpin nucleotide for HCR reaction; MB@SA, streptavidin-coated magnetic beads; NI1–2, nucleotide initiator; SP1–2, signal probe. Reprinted and adapted from [ 36 ] with permission.…”