An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced acetycholinesterase activity

Abstract

There has been urgent demand for rapid, sensitive and cost-effective pesticide assay technologies due to the global attention of environmental and food-safety problems. Acetycholinesterase (AChE)-based electrochemical sensors have attracted significant interest toward this goal. In this contribution, we introduced multiwalled carbon nanotubes (MWNTs) into our sensor design, where they played dual enhancement roles; first is that MWNTs loaded on glassy carbon (GC) electrodes significantly increase surface areas, facilitating the electrochemical polymerization of prussian blue (PB), a redox mediator for the electrochemical oxidation of the enzymatic product, thiocholine (TCh). Second, MWNTs enhance the enzymatic activity of AChE, as manifested by the decreased Michaelis-Menten constant (K(m)). As a result of these two important enhancement factors offered by MWNTs, our electrochemical pesticide sensor exhibited rapid response and high sensitivity toward the detection of a series of pesticides. Moreover, we demonstrated that this sensor was stable, reproducible and selective enough for detection in real samples.

Immobilization of acetylcholineesterase–choline oxidase on a gold–platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents

“…Amperometric biosensors based on inhibition of these have shown high sensitivity and good results for pesticides and OP compounds in which decrease in the enzyme activity is monitored as quantitative measurements of the pesticides and OP compounds (Amine et al, 2006;Chen et al, 2008;Kok et al, 2002). The biocompatibility of AChE on gold nanoparticles coated silicon nanowires, has shown the satisfactory result for pesticide detection .…”

Nanomaterials Based Electrochemical Sensing Applications for Safety and Security

“…CNTs have been used as electrochemical platforms for several applications such as for microbiological [18][19][20][21][22]25], toxins [59,60], pesticides [31,[38][39][40][41][42][43][44][45][46][47][48][49][50][51][52] and explosives [64][65][66] detections.…”

Colorimetric detection and efficient monitoring of a potential biomarker of lumbar disc herniation using carbon nanotube-based probe

“…Therefore, nanoprobes could efficiently amplify detection signals and easily achieve ultrahigh detection limit. As we know, carbon nanotubes (CNTs) possess an extremely large surface area, which could easily form biomolecular conjugation to amplify detection signals [26][27][28][29]. In this paper, we extended the application of MWNT-based colorimetric probe for ApoL1 detection.…”